The European Network for Diagnostics of Imported Viral Diseases (ENIVD) – 12 years of strengthening the laboratory diagnostic capacity in Europe

Niedrig, Matthias; Donoso-Mantke, Oliver; Schädler, R; members, Enivd

doi:10.2807/esw.12.16.03180-en

Cited by 6 publications

(5 citation statements)

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“…The European Network for the Diagnostics of ‘Imported’ Viral Diseases -Collaborative Laboratory Response Network (ENIVD-CLRN) established and coordinated this EQA as in other EQAs previously performed [9] – [12] .…”

Section: Methodsmentioning

confidence: 99%

First International External Quality Assessment Study on Molecular and Serological Methods for Yellow Fever Diagnosis

et al. 2012

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ObjectiveWe describe an external quality assurance (EQA) study designed to assess the efficiency and accurateness of molecular and serological methods used by expert laboratories performing YF diagnosis.Study DesignFor molecular diagnosis evaluation, a panel was prepared of 14 human plasma samples containing specific RNA of different YFV strains (YFV-17D, YFV South American strain [Brazil], YFV IvoryC1999 strain), and specificity samples containing other flaviviruses and negative controls. For the serological panel, 13 human plasma samples with anti-YFV-specific antibodies against different strains of YFV (YFV-17D strain, YFV IvoryC1999 strain, and YFV Brazilian strain), as well as specificity and negative controls, were included.ResultsThirty-six laboratories from Europe, the Americas, Middle East, and Africa participated in these EQA activities. Only 16% of the analyses reported met all evaluation criteria with optimal performance. Serial dilutions of YFV-17D showed that in general the methodologies reported provided a suitable sensitivity. Failures were mainly due to the inability to detect wild-type strains or the presence of false positives. Performance in the serological diagnosis varied, mainly depending on the methodology used. Anti-YFV IgM detection was not performed in 16% of the reports using IIF or ELISA techniques, although it is preferable for the diagnosis of YFV acute infections. A good sensitivity profile was achieved in general; however, in the detection of IgM antibodies a lack of sensitivity of anti-YFV antibodies against the vaccine strain 17D was observed, and of the anti-YFV IgG antibodies against a West African strain. Neutralization assays showed a very good performance; however, the unexpected presence of false positives underlined the need of improving the running protocols.ConclusionThis EQA provides information on each laboratory's efficacy of RT-PCR and serological YFV diagnosis techniques. The results indicate the need for improving serological and molecular diagnosis techniques and provide a follow-up of the diagnostic profiles.

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Section: Methodsmentioning

confidence: 99%

First International External Quality Assessment Study on Molecular and Serological Methods for Yellow Fever Diagnosis

et al. 2012

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“…For these reasons, an EQA study for the molecular diagnosis of LASV was conducted by the European Network for Diagnostics of ‘Imported’ Viral Diseases (ENIVD) ( http://www.enivd.org ) in 2013. ENIVD is concerned with the development of laboratory diagnostic capacities for imported virus infections, quality control, standardization of laboratory procedures, and training of laboratory staff [ 32 ]. Based on the results of this study, the quality of LASV diagnostics may be improved.…”

Section: Introductionmentioning

confidence: 99%

International External Quality Assessment Study for Molecular Detection of Lassa Virus

Nikisins

Rieger²,

Patel³

et al. 2015

PLoS Negl Trop Dis

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Lassa virus (LASV) is a causative agent of hemorrhagic fever in West Africa. In recent years, it has been imported several times to Europe and North America. The method of choice for early detection of LASV in blood is RT-PCR. Therefore, the European Network for Diagnostics of ‘Imported’ Viral Diseases (ENIVD) performed an external quality assessment (EQA) study for molecular detection of LASV. A proficiency panel of 13 samples containing various concentrations of inactivated LASV strains Josiah, Lib-1580/121, CSF, or AV was prepared. Samples containing the LASV-related lymphocytic choriomeningitis virus (LCMV) and negative sera were included as specificity controls. Twenty-four laboratories from 17 countries (13 European, one African, one Asian, two American countries) participated in the study. Thirteen laboratories (54%) reported correct results, 4 (17%) laboratories reported 1 to 2 false-negative results, and 7 (29%) laboratories reported 3 to 5 false-negative results. This EQA study indicates that most participating laboratories have a good or acceptable performance in molecular detection of LASV. However, several laboratories need to review and improve their diagnostic procedures.

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“…Quality assurance panels may be helpful here similar to what has been done by the European Network for Diagnostics of Imported Viral Diseases for diagnostics of emerging pathogens. 17…”

mentioning

confidence: 99%

Gamma Irradiation as an Effective Method for Inactivation of Emerging Viral Pathogens

Feldmann

Shupert

Haddock

et al. 2019

The American Journal of Tropical Medicine and Hygiene

129

130

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Gamma irradiation using a cobalt-60 source is a commonly used method for the inactivation of infectious specimens to be handled safely in subsequent laboratory procedures. Here, we determined irradiation doses to safely inactivate liquid proteinaceous specimens harboring different emerging/reemerging viral pathogens known to cause neglected tropical and other diseases of regional or global public health importance. By using a representative arenavirus, bunyavirus, coronavirus, filovirus, flavivirus, orthomyxovirus, and paramyxovirus, we found that these enveloped viruses differed in their susceptibility to irradiation treatment with adsorbed doses for inactivation of a target dose of 1 × 10 6 50% tissue culture infectious dose (TCID 50 )/mL ranging from 1 to 5 MRads. This finding seemed generally inversely correlated with genome size. Our data may help to guide other facilities in testing and verifying safe inactivation procedures.Efficient and reliable inactivation of specimens is an important component of many laboratory operations, but especially critical for biocontainment laboratories handling emerging/reemerging pathogens causing neglected tropical infectious diseases of epidemic as well as infectious disease of regional and global (pandemic) public health importance. Multiple methods can be applied following approved safety testing by institutional and national authorities including those based on chemical, heat, and radiation treatment.

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The European Network for Diagnostics of Imported Viral Diseases (ENIVD) – 12 years of strengthening the laboratory diagnostic capacity in Europe

Cited by 6 publications

References 0 publications

First International External Quality Assessment Study on Molecular and Serological Methods for Yellow Fever Diagnosis

First International External Quality Assessment Study on Molecular and Serological Methods for Yellow Fever Diagnosis

International External Quality Assessment Study for Molecular Detection of Lassa Virus

Gamma Irradiation as an Effective Method for Inactivation of Emerging Viral Pathogens

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