The evaluation of a phage-typing system for Listeria monocytogenes for use in epidemiological studies

McLauchlin, James; Audurier, A; Taylor, A. G.

doi:10.1099/00222615-22-4-357

Cited by 63 publications

(32 citation statements)

References 14 publications

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“…1985). Interpretation of phage typing results was performed on the basis that strains which differed by more than one (strains of serotype 1/2) or two (strains of serotype 4) major difference(s) in lysis reactions belonged to different phage types (McLauchlin et al . 1986).…”

Section: Methodsmentioning

confidence: 99%

Comparison of sludge and clinical isolates of Listeria monocytogenes

Lozniewski

Humbert

Corsaro

et al. 2001

Lett Appl Microbiol

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Section: Methodsmentioning

confidence: 99%

Comparison of sludge and clinical isolates of Listeria monocytogenes

Lozniewski

Humbert

Corsaro

et al. 2001

Lett Appl Microbiol

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“…A serotyping scheme developed for species of Listeria on the basis of variation in the somatic (0) and flagellar (H) antigens (9) is of limited use because most clinical isolates of L. monocytogenes represent only three serotypes (4b, 1/2a, and 1/2b) (2,10). A phage typing scheme is also available (11,12), but only 60 to 70% of isolates of L. monocytogenes are typeable (2,12); and phage typing does not provide estimates of genetic relatedness among strains. Multilocus enzyme electrophoresis has recently been employed to study the genetic structure and epidemiology of a number of pathogenic bacteria of various genera (13)(14)(15) (13).…”

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confidence: 99%

Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease.

Piffaretti¹,

Kressebuch²,

Aeschbacher³

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

308

252

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One hundred and seventy-five isolates of the pathogenic bacterium Listeria monocytogenes recovered from human clinical (blood and cerebrospinal fluid), animal, and environmental sources in Europe, North America, and elsewhere were analyzed electrophoretically for allelic variation at 16 genetic loci encoding metabolic enzymes. Forty-five distinctive allele profiles (electrophoretic types, ETs) were distinguished, among which mean genetic diversity per locus (H) was 0.424. Cluster analysis of a matrix of genetic distances between paired ETs revealed two primary phylogenetic divisions of the species separated at a distance of 0.54. ETs in division I were presented by strains of serotypes 4b, 1/2b, and 4a, whereas strains of ETs in division II were of serotypes 1/2a and 1/2c.Human and animal isolates did not represent distinctive subsets of ETs. The occurrence of linkage disequilibrium between enzyme loci and the widespread distribution of certain ETs indicate that the genetic structure ofL. monocytogenes is clonal. One clone, marked by ET 1, caused major epidemics of human disease in western Switzerland in the period -1987 and in Los Angeles County, California, in 1985, both of which were attributed to contamination of soft cheese. ET 1 is closely related to the clone (ET 7) that caused two large outbreaks of listeriosis in Massachusetts in 1979 and Bacteria of the genus Listeria are widely distributed in the environment and also occur in the intestinal tract of healthy animals and humans (1). Among the several species of the genus, only Listeria monocytogenes is commonly pathogenic for humans (2, 3), in which it causes serious invasive disease (septicemia, meningitis, and meningoencephalitis), primarily in the immunologically compromised host, the neonate, and the fetus (1,2 3818The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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“…Traditional subtyping methods include serotyping (34) and phage typing (23,24). Serotyping is of restricted value because most human clinical L. monocytogenes isolates belong to only 3 (1/2a, 1/2b, and 4b) of the 13 serotypes known for this species (11,22,32,33).…”

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confidence: 99%

Automated Ribotyping Using Different Enzymes To Improve Discrimination ofListeria monocytogenesIsolates, with a Particular Focus on Serotype 4b Strains

et al. 2001

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To develop improved automated subtyping approaches for Listeria monocytogenes, we characterized the discriminatory power of different restriction enzymes for ribotyping. When 15 different restriction enzymes were used for automated ribotyping of 16 selected L. monocytogenes isolates, the restriction enzymes EcoRI, PvuII, and XhoI showed high discriminatory ability (Simpson's index of discrimination > 0.900) and produced complete and reproducible restriction cut patterns. These three enzymes were thus evaluated for their ability to differentiate among isolates representing the two major serotype 4b epidemic clones, those having ribotype reference pattern DUP-1038 (51 isolates) and those having pattern DUP-1042 (20 isolates). Among these isolates, PvuII provided the highest discrimination for a single enzyme (nine different subtypes; index of discrimination ‫؍‬ 0.518). A combination of PvuII and XhoI showed the highest discriminatory ability (index of discrimination ‫؍‬ 0.590) for these isolates. A group of 44 DUP-1038 isolates and a group of 12 DUP-1042 isolates were identical to each other even when the combined data for all three enzymes were used. We conclude that automated ribotyping using different enzymes allows improved discrimination of L. monocytogenes isolates, including epidemic serotype 4b strains. We furthermore confirm that most of the isolates representing the genotypes linked to the two major epidemic L. monocytogenes clonal groups form two genetically homogeneous groups.

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The evaluation of a phage-typing system for Listeria monocytogenes for use in epidemiological studies

Cited by 63 publications

References 14 publications

Comparison of sludge and clinical isolates of Listeria monocytogenes

Comparison of sludge and clinical isolates of Listeria monocytogenes

Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease.

Automated Ribotyping Using Different Enzymes To Improve Discrimination ofListeria monocytogenesIsolates, with a Particular Focus on Serotype 4b Strains

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