The Evaluation of Antimicrobial and Antibiofilm Activity of Bioactive Compounds Obtained from Aspergillus Sclerotiorum

Abstract

This study was concerned with the screening of antimicrobial products from fungi collected from soil and evalution of their antibiofilm activity. The isolate having antimicrobial and antibiofilm compounds was characterized by the molecular methods and identified as Aspergillus sclerotiorum. A. sclerotiorum was grown in yeast peptone glucose (YPG) medium and extracellular medium was extraction by 1:1 ethyl acetate. Crude extraction characterized through thin layer chromatography (TLC) on silica gel 60 HF254 and was detected five bands. Agar diffusion and TLC overlay assays were done against Gram-positive (Staphylococcus aureus ATCC 25923, meticilin resistance S. aureus (MRSA) and Enterococcus faecalis ATCC 29212) and Gram-negative bacteria (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853). The most dense band (Rf:0.42) showed the best inhibition zone on TLC overlay. The results showed that the most dense band can potential source for antimicrobial compound. After the most dense band in silica gel was scraped and dissolved ethyl acetate for minimum inhibitory concentration (MIC) determination and crystal violet assay against S. aureus and MRSA. These results indicate that fungi, A. sclerotiorum, isolated from soil was potential source for antimicrobial and antibiofilm compounds.

Antimicrobial and antibiofilm activity of Ferula spp. growing in Erzurum

“…An absorbance measurement was taken at 590 nm. The evaluation was made according to negative and positive control (17).…”