The evaluation of cyclic uniaxial strain on myogenic differentiation of adipose-derived stem cells

Bayati, Vahid; Sadeghi, Yousef; Shokrgozar, Mohammad Ali; Haghighipour, Nooshin; Azadmanesh, Kayhan; Amanzadeh, Amir; Azari, Shahram

doi:10.1016/j.tice.2011.07.004

Cited by 43 publications

(41 citation statements)

References 35 publications

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“…Balata et al [30] showed that stretch alone could induce myogenic differentiation in rat ASCs. The authors demonstrated that cyclic uniaxial strain (10%, 1Hz) for 24 hours increased the gene expression of myogenic markers (Myod, Myog and Myh2), and this pattern was even greater when associated with chemical factors.…”

Section: Resultsmentioning

confidence: 99%

Short-term mechanical stretch fails to differentiate human adipose-derived stem cells into cardiovascular cell phenotypes

et al. 2014

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BackgroundWe and others have previously demonstrated that adipose-derived stem cells (ASCs) transplantation improve cardiac dysfunction post-myocardium infarction (MI) under hemodynamic stress in rats. The beneficial effects appear to be associated with pleiotropic factors due to a complex interplay between the transplanted ASCs and the microenvironment in the absence of cell transdifferentiation. In the present work, we tested the hypothesis that mechanical stretch per se could change human ASCs (hASCs) into cardiovascular cell phenotypes that might influence post-MI outcomes.MethodsHuman ASCs were obtained from patients undergoing liposuction procedures. These cells were stretched 12%, 1Hz up to 96 hours by using Flexercell 4000 system. Protein and gene expression were evaluated to identify cardiovascular cell markers. Culture medium was analyzed to determine cell releasing factors, and contraction potential was also evaluated.ResultsMechanical stretch, which is associated with extracellular signal-regulated kinase (ERK) phosphorylation, failed to induce the expression of cardiovascular cell markers in human ASCs, and mesenchymal cell surface markers (CD29; CD90) remained unchanged. hASCs and smooth muscle cells (SMCs) displayed comparable contraction ability. In addition, these cells demonstrated a profound ability to secrete an array of cytokines. These two properties of human ASCs were not influenced by mechanical stretch.ConclusionsAltogether, our findings demonstrate that hASCs secrete an array of cytokines and display contraction ability even in the absence of induction of cardiovascular cell markers or the loss of mesenchymal surface markers when exposed to mechanical stretch. These properties may contribute to beneficial post-MI cardiovascular outcomes and deserve to be further explored under the controlled influence of other microenvironment components associated with myocardial infarction, such as tissue hypoxia.

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Section: Resultsmentioning

confidence: 99%

Short-term mechanical stretch fails to differentiate human adipose-derived stem cells into cardiovascular cell phenotypes

et al. 2014

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“…It should be mentioned that all experiments were performed in accordance with the protocols approved by the Institutional Animal Care and Use Committee and with the guidelines for care and use of experimental animals required by AJUMS. After rats were sacrificed, their gonadal fat pads were excised, and rat ASCs were isolated using a published method (12). Briefly, the adipose tissue was carefully dissected and digested using 0.1% collagenase type I (Gibco, USA) for 50 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…These include soluble growth factors and cytokines (10, 11), mechanical stimuli (12), substrate properties (13), and culture conditions (14). Initial cell seeding density as one of the culture conditions, has been indicated to have tremendous effect on cell proliferation, differentiation, and extracellular matrix (ECM) synthesis (15–21).…”

Section: Introductionmentioning

confidence: 99%

Impact of Cell Density on Differentiation Efficiency of Rat Adipose-derived Stem Cells into Schwann-like Cells

Bayati

Orazizadeh

et al. 2016

IJSC

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Background and ObjectivesSchwann-like (SC-like) cells induced from adipose-derived stem cells (ASCs) may be one of the ideal alternative cell sources for obtaining Schwann cells (SCs). They can be used for treating peripheral nerve injuries. Co-culture with SCs or exposure to glial growth factors are commonly used for differentiation of ASCs to SC-like cells. However, the effect of initial cell density as an inductive factor on the differentiation potential of ASCs into the SC-like cells has not been yet investigated.Methods and ResultsASCs were harvested from rat and characterized. The cells were seeded into the culture flasks at three different initial cell densities i.e. 2×103, 4×103 and 8×103 cells/cm2 an overnight and differentiated toward SC-like cells using glial growth factors. After two weeks, the differentiation rate of ASCs to SC-like cells at different densities was assessed by immunofluorescence, fluorescence-activated cell sorting analysis and real time RT-PCR. Expression of the typical SCs markers, S-100 proteins and glial fibrillary acidic protein (GFAP) protein, was observed in all cell densities groups although the number of S100-positive and GFAP-positive cells, and the expression of p75NTR mRNA, another SC marker, were significantly higher at the density of 8×103 cells/cm2 when compared with the other cell densities groups (p<0.001).ConclusionsThe results suggest that the higher differentiation rate of ASCs to SC-like cells can be obtained at initial cell density of 8×103 cells/cm2, possibly via increased cell-cell interaction and cell density-dependent influence of glial growth factors.

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“…Mechanical stress is one of the known factors inducing ADMSCs differentiation into muscular or endothelial cells, although short-term mechanical factors alone are not sufficient [42,43]. Deep filler placement, however, results in a prolonged mechanical stress.…”

Section: A New Concept Of ''Dermal'' Filler Effects -Adipose Tissue Smentioning

confidence: 99%

Midfacial rejuvenation by hyaluronic acid fillers and subcutaneous adipose tissue – A new concept

Wollina

2015

Medical Hypotheses

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The evaluation of cyclic uniaxial strain on myogenic differentiation of adipose-derived stem cells

Cited by 43 publications

References 35 publications

Short-term mechanical stretch fails to differentiate human adipose-derived stem cells into cardiovascular cell phenotypes

Short-term mechanical stretch fails to differentiate human adipose-derived stem cells into cardiovascular cell phenotypes

Impact of Cell Density on Differentiation Efficiency of Rat Adipose-derived Stem Cells into Schwann-like Cells

Midfacial rejuvenation by hyaluronic acid fillers and subcutaneous adipose tissue – A new concept

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