The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate

Çelık, Ayla; Őğenler, Oya; Çömelekoğlu, Ülkü

doi:10.1093/mutage/gei055

Cited by 73 publications

(27 citation statements)

References 44 publications

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“…Formation of micronuclei containing chromosome fragments or whole chromosomes, which are indicative of cytogenetic damage in peripheral blood erythrocyte, was not observed in any of the groups following administration of the Streptococcus phocae (n = 8). Briefly, immature erythrocytes were identified by their orange-red color, mature erythrocytes by their green color, and micronuclei by their yellowish color as stated by Celik et al [4]. It is evident from the Fig.…”

Section: Resultsmentioning

confidence: 99%

Biopreservation of Sardinella longiceps and Penaeus monodon Using Protective Culture Streptococcus phocae PI 80 Isolated from Marine Shrimp Penaeus indicus

Paari¹,

Kanmani²,

Satishkumar³

et al. 2011

Probiotics & Antimicro. Prot.

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The influence of Streptococcus phocae PI80 on the shelf life of Sardinella longiceps and Penaeus monodon was investigated by measurement of microbial and chemical analysis after appraising the safety of the protective probiotic culture in wistar rat's model. The results of this safety assessment indicate that oral administration of protective culture does not demonstrate any toxicological effects. Consumption of this LAB strain had no adverse effects on animal's general health status, hematology, blood biochemistry, histology parameters, or on the incidence of bacterial translocation. The effect of Streptococcus phocae is very evident with the reduction of Listeria monocytogenes, Vibrio parahemolyticus, and coliforms. During storage, a marked decline in total volatile base and peroxide value was observed in protective culture-treated samples than the control. This strain looks promising as a protective culture for the preservation of fish products.

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Section: Resultsmentioning

confidence: 99%

Biopreservation of Sardinella longiceps and Penaeus monodon Using Protective Culture Streptococcus phocae PI 80 Isolated from Marine Shrimp Penaeus indicus

Paari¹,

Kanmani²,

Satishkumar³

et al. 2011

Probiotics & Antimicro. Prot.

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“…Effects of chronic exposure of laboratory mice to DCG were examined in this study. The mouse bone marrow micronucleus (MN) assay, a predictive index for evaluating the carcinogenic potential of environmental and occupational chemical exposure was used [16,17]. Our results clearly indicate that all the three doses of DCG studied significantly (P < 0.05) induced the formation of micronuclei in the polychromatic erythrocytes (PCEs) of the mice bone marrow cells (Table 3).…”

Section: Discussionmentioning

confidence: 82%

Exposure of Laboratory Mice to Domestic Cooking Gas: - Implications for Toxicity

Odunola

2008

Int. J. Environ. Res. Public Health

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Abstract:The ability of domestic cooking gas to induce hepatotoxicity and clastogenicity in mice was studied. The mice were exposed to domestic gas for twenty-one days at doses of 100 mg/kg, 200 mg/kg and 300 mg/kg respectively. The positive control group of mice were given sodium arsenite intraperitoneously at a dose of 2.5mg/kg body weight. While the negative control group had only distilled water, sodium arsenite significantly (p < 0.05) induced the formation of micronucleated polychromatic erythrocytes (mPCEs), serum and liver gamma glutamyl transferase (γGT) and alkaline phosphatase (AP) activities respectively as compared with the observations made in the negative control group. Similarly, the domestic gas significantly (p<0.05) induced mPCEs formation, serum and liver, γGT and AP activities. The degree of induction was in the order of 100 mg/kg < 200 mg/kg < 300 mg/kg. However, when compared with the positive control group, the domestic cooking gas at the tested doses was not as potent as sodium arsenite in its ability to induce enzyme activity and mPCEs formation. Limited histopathological analysis of liver samples from treated and untreated mice showed distended blood vessels, necrosis and hepatocellular degeneration in the groups treated with high doses of domestic gas or sodium arsenite as compared with the untreated group. Our findings suggest that the domestic cooking gas has some degree of clastogenic and hepatotoxic activities in mice. Health risks may therefore be associated with long-term occupational and / or domestic exposure in humans.

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“…Genotoxicity assays such as the comet assay were carried out with peripheral blood leukocytes (PBL) and liver cells (Kumari, Kumari, Kamal, & Grover, ). Micronucleus test (MNT) was performed in peripheral blood (PB) cells (Çelik, Öğenler, & Çömelekoğlu, ) and bone marrow cells as per OECD guideline 474 (OECD, 2014). To estimate the alterations in biochemical indices such as Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), superoxide dismutase (SOD), catalase (CAT), glutathione content (GSH), lipid peroxidation (LPO) and lactate dehydrogenase (LDH) levels were evaluated in serum, liver and kidney tissues of treated rats.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genotoxicity and biodistribution of nano‐ and micron‐sized yttrium oxide in rats after acute oral treatment

Panyala

Chinde

Kumari

et al. 2017

J of Applied Toxicology

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The increasing use of yttrium oxide (Y O ) nanoparticles (NPs) entails an improved understanding of their potential impact on the environmental and human health. In the present study, the acute oral toxicity of Y O NPs and their microparticles (MPs) was carried out in female albino Wistar rats with 250, 500 and 1000 mg kg body weight doses. Before the genotoxicity evaluation, characterization of the particles by transmission electron microscopy, dynamic light scattering and laser Doppler velocimetry was performed. The genotoxicity studies were conducted using micronucleus and comet assays. Results showed that Y O NP-induced significant DNA damage at higher dose (1000 mg kg body weight) in peripheral blood leukocytes and liver cells, micronucleus formation in bone marrow and peripheral blood cells. The findings from biochemical assays depicted significant alterations in aspartate transaminase, alanine transaminase, alkaline phosphatase, malondialdehyde, superoxide dismutase, reduced glutathione, catalase and lactate dehydrogenase levels in serum, liver and kidneys at the higher dose only. Furthermore, tissue biodistribution of both particles was analyzed by inductively coupled plasma optical emission spectrometry. Bioaccumulation of yttrium (Y) in all tissues was significant and dose-, time- and organ-dependent. Moreover, Y O NP-treated rats exhibited higher tissue distribution along with greater clearance through urine whereas Y O MP-dosed animals depicted the maximum amount of Y in the feces. Hence, the results indicated that bioaccumulation of Y O NPs via its Y ions may induce genotoxic effects.

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The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate

Cited by 73 publications

References 44 publications

Biopreservation of Sardinella longiceps and Penaeus monodon Using Protective Culture Streptococcus phocae PI 80 Isolated from Marine Shrimp Penaeus indicus

Biopreservation of Sardinella longiceps and Penaeus monodon Using Protective Culture Streptococcus phocae PI 80 Isolated from Marine Shrimp Penaeus indicus

Exposure of Laboratory Mice to Domestic Cooking Gas: - Implications for Toxicity

Assessment of genotoxicity and biodistribution of nano‐ and micron‐sized yttrium oxide in rats after acute oral treatment

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