The evaluation of nitric oxide scavenging activity of certain herbal formulations in vitro: a preliminary study

Abstract

The nitric oxide (NO) scavenging activities of traditional polyherbal drugs like abana, chyavanaprasha, geriforte, septilin, mentat and triphala were examined using sodium nitroprusside as a NO donor in vitro. All the drugs tested demonstrated direct scavenging of NO and were superior to Gingko biloba, which was used as a positive control. The extracts of various polyherbal drugs exhibited dose-dependent NO scavenging activities and the potency was in the following order: abana > chyavanaprasha > triphala > geriforte > septilin > mentat > Gingko biloba. The present results suggest that the traditional Indian polyherbal crude drugs may be potent and novel therapeutic agents for scavenging of NO, and thereby inhibit the pathological conditions caused by excessive generation of NO and its oxidation product, peroxynitrite. These findings may also help to explain, at least in part, the pharmacological activities like rejuvenating, adaptogenic, anti-infection, anti-inflammatory, cardioprotective and neuroprotective activities of these traditional, clinically used non toxic drugs.

Antioxidant Properties of the Leaves of Chromolaena odorata Linn

“…It could be due to diverse phytochemicals including the phenolic compounds in the plant. Phenolic compounds have been implicated in antioxidant metabolism 15 DQG WKH QLWULF R[LGH VFDYHQJLQJ DFWLYLW\ RI ÀDYRQRLGV DQG SKHQROLF FRPSRXQGV are known 16,17 .…”

Evaluation of In vitro Antioxidant Properties of Hydro Alcoholic Extract of Entire Plant of Cynodon dactylon

“…NO and its derivatives produced by the activated phagocytes might have genotoxic effect contributing in the multistage carcinogenesis process. 31,32 It would be interesting to develop potent and selective inhibitors of NO for potential therapeutic use In our study the scavenging activity of plant extract at the minimum concentration of 20 µg and maximum concentration of 100 µg was found to be 7.01% and 41.8%. Whereas, standard Rutin was 45.8% and 74.3% respectively ( Figure 5).…”

Herbal Antioxidants as Rejuvenators in Alternative Medicine

“…Chebulagic acid isolated from Terminalia chebula has been reported to inhibit COX-2/5-LOX (Reddy et al, 2010). Apart from this, triphala has been shown to possess significant antioxidant (Naik et al, 2005;Mahesh et al, 2009;Hazra et al, 2010) and nitric oxide scavenging activity (Jagetia et al, 2004). In addition, the anti-cancer (Deep et al, 2005;Sandhya and Mishra, 2006;Shi et al, 2008), immunomodulatory (Srikumar et al, 2005) and anti-inflammatory (Rasool and Sabina, 2007) activity of triphala directly targets the common health problems associated with aging.…”