The evaluation of oxidative stress in patients with essential thrombocythemia treated with risk-adapted therapy

Moisă, Cornel; Găman, Mihnea‐Alexandru; ASSANI, ALEXANDRU DAN; Găman, Amelia-Maria; Pharmacy, Bucharest

doi:10.31688/abmu.2018.53.4.07

Cited by 19 publications

(23 citation statements)

References 13 publications

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“…The JAK2V617F mutation was positive in 36 ET patients (58.06%) (heterozygous genotype in 30 patients and homozygous genotype in six patients) and negative in 26 ET patients (41.94%), according with literature data. ROS levels were increased (with high levels in JAK2V617F-positive ET patients vs. JAK2V617F-negative cases; mean ROS value = 2.73 mM/L vs. 2.60 mM/L; p<0.05) and TAC levels were decreased in patients with ET vs. the control group (with low levels in JAK2V617F-positive patients vs. JAK2V617Fnegative patients, mean TAC value = 0.46 mM/L vs. 0.51 mM/L; p<0.05) [12,15].…”

Section: Resultsmentioning

confidence: 90%

“…Low-grade chronic inflammation increases genomic instability and clonal evolution to myelofibrosis and leukemic transformation [7][8]. On the other hand, overproduction of ROS enhances myeloid proliferation with leukocytosis, thrombocytosis, inflammation-mediated activation of leukocytes and platelets, continuous release of inflammatory products and a self-perpetuating vicious circle involving excessive ROS, growth advantaged JAK2V617F-positive clones, proliferation of megakaryocytes, genomic instability and disease progression [9][10][11][12][13][14][15].…”

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confidence: 99%

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Crosstalk between Oxidative Stress, Chronic Inflammation and Disease Progression in Essential Thrombocythemia

Găman¹,

Moisă²,

Diaconu³

et al. 2019

Rev. Chim.

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Essential thrombocythemia (ET) is a Philadelphia-negative chronic myeloproliferative neoplasm characterized by acquired somatic mutations: JAK2, CALR or MPL. It is associated with low-grade chronic inflammation, oxidative stress, overproduction of reactive oxygen species (ROS) and antioxidant deficiency. In ET, chronic inflammation and oxidative stress contribute to the genomic instability, the clonal evolution to myelofibrosis and the leukemic transformation. We evaluated ROS levels and the total antioxidant capacity (TAC) in 62 ET patients and investigated the relationship between ROS, TAC, chronic inflammation, leukocytosis, JAK2V617F mutation, and disease progression to myelofibrosis or leukemic transformation. We observed increased levels of ROS and inflammation markers and a decreased TAC in ET patients vs. controls. The acute myeloid leukaemia transformation associated increased levels of oxidative stress and inflammation markers and increased leukocyte counts, while myelofibrosis progression associated an increase in ROS and serum ferritin.

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Section: Resultsmentioning

confidence: 90%

mentioning

confidence: 99%

Crosstalk between Oxidative Stress, Chronic Inflammation and Disease Progression in Essential Thrombocythemia

Găman¹,

Moisă²,

Diaconu³

et al. 2019

Rev. Chim.

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“…It is noteworthy to mention that, in susceptible individuals, including obese subjects, increased levels of oxidative stress and inflammation may contribute to an increased risk of developing solid and haematological cancers [32][33][34].…”

Section: Fig 1 Distribution Of the Study Group Based On Obesity Clamentioning

confidence: 99%

Crosstalk between Oxidative Stress and Inflammation in Obesity

Epîngeac¹,

Găman²,

Diaconu³

et al. 2020

Rev. Chim.

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Obesity is a multifactorial disease in which oxidative stress and inflammation play important roles. The aim of our study was to evaluate oxidative stress levels and inflammation markers in obese subjects vs. controls and to investigate the relationship between these values. We found increased levels of reactive oxygen species and inflammation markers (fibrinogen, ferritin, CRP, NLR) and decreased levels of antioxidants in obese subjects vs. controls.Obesity is a multifactorial disease, characterized by the accumulation of abnormal or excessive fat. Considered a global health problem, obesity is involved in the development of a myriad of health issues, such as diabetes mellitus, cardiovascular disease, cancer, infertility, asthma, sleep disorders, irritable bowel syndrome, hepatic and renal dysfunctions. According to the World Health Organization (WHO) definition of overweight and obesity, an overweight subject has a body mass index (BMI) of 25 to 29.9 kg/m 2 , whereas the BMI of an obese subject exceeds 30 kg/m 2 . Recent studies have underlined that, in obese subjects, the adipose tissue dysfunction derives also from the interplay between reactive oxygen species (ROS) signalling and inflammatory response pathways [1][2][3][4][5][6][7][8][9].Oxidative stress, defined as the imbalance between the production of ROS and the levels of antioxidants in the body, is a key element in the development of obesity and many obesity-related comorbidities. The main ROS involved in this process are the superoxide anion • O2 -, the hydrogen peroxide H2O2 and the hydroxyl radical • OH, whereas antioxidant enzymes, i.e. superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), uric acid, cofactors (e.g. NADPH), vitamin E, vitamin C, thioredoxin and glutathione (GSH) and trace metals (e.g. selenium) counteract pro-oxidant effects of ROS. Oxidative stress can also be induced by obesity per se by several mechanisms such as oxidative phosphorylation in the mitochondria, oxidation of fatty acids, over-consumption of oxygen, protein kinase C activation, hyperleptinemia, postprandial ROS generation due to lipid-rich and carbohydrate-rich diets, low antioxidant defenses, endothelial dysfunction due to reduced bioavailability of nitric oxide and increased levels of endothelium-derived contractile factors, and chronic inflammation [10][11][12][13][14][15][16][17][18].In the adipose tissue, local hypoxia and the hypertrophy of adipocytes resulted during the overgrowth of fat cells can act as inflammation triggers. Hypoxia is correlated with the macrophage infiltration into the fat tissue and with an excess of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). Thus, in the adipose tissue, low-grade localized inflammation can develop and then lead to systemic inflammation which acts as an essential element in the development of many obesityassociated comorbidities [9]. TNF-α is a pro-inflammatory cytokine which binds to specific receptors and is involved in immunity, adipose cell apoptosis, lipid metabolism, insulin signallin...

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“…thrombocythemia and idiopathic myelofibrosis are less frequent causes 7 . Hyperhomocysteinemia and methylene-tetrahydrofolate reductase gene mutations (MTHFR) are less important risk factors for splanchnic thrombosis 8 .…”

Section: Etiologymentioning

confidence: 99%