The Evaluation of Potential Skin Sensitization and Collagen Synthesis of Rats Receiving Proteoglycan Serum

Chuenwattana, Wannara; Chatthanawaree, Wichai; Bunman, Sitthiphon; Yoshikawa, Hiroshi

doi:10.31524/bkkmedj.2018.02.001

Cited by 1 publication

(3 citation statements)

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“…This study was investigated using the method of Chuenwattana et al (2018). 3 Skin tissue sections were deparaffinized in xylene, hydrated, and stained in Weigert's iron hematoxylin solution for 10 minutes. They were washed and stained in acid fuchsin solution for 3 minutes, rinsed in distilled water, treated with phosphomolybdicphosphotugstic acid solution for 15 minutes, immediately submerged into aniline blue solution for 10 minutes, rinsed in distilled water, treated with acetic solution for 10 minutes, dehydrated in 95% alcohol, cleared twice in xylene, mounted with a cover slip, and observed under a light microscope.…”

Section: The Masson's Trichrome Examinationmentioning

confidence: 99%

“…The study demonstrated that this serum has accelerated collagen synthesis and low potential skin sensitization in rat models. 3 In 2019, Bunman developed and formulated a new cream for facial use, called "bright and firm hybrid emulsion I". Bright and firm hybrid emulsion I is composed of many active ingredients such as pseudoalteromonas ferment extract, chlamydomonas extract, hamamelis virginiana (witch hazel) extract, and astaxanthin etc.…”

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confidence: 99%

“…(2015)1, which was modified fromChuenwattana et al (2018).2 Male Wistar rats (age 8 weeks, weighing 250-300 grams) were purchased from the national Laboratory Animal Centre, Mahidol University, Salaya, Thailand. Rats were housed in the Laboratory Animal Unit under standard conditions of temperature 25 ± 2 °C, 50 -60 % humidity, and a 12 hours/12 hours light/dark cycle.…”

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Evaluation of the Antioxidant Activity, Collagen Synthesis and Stability of Bright and Firm hybrid emulsion I

Bunman¹,

Chinjarernpan²,

Stitsmith³

et al. 2019

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In 2011, Bunman et al., extracted proteoglycan from the head of fish cartilage and developed cream extraction from the head of fish cartilage. Proteoglycan is a hybrid molecule composed of a central core protein by bonding it with polysaccharides (glycosaminoglycans or GAGs) with a covalent bond, previously called mucopolysaccharide due to its physical property (jelly-like, sticky and viscosity). GAGs can be divided into 5 groups: chrondroitin/chorndroitan sulfate, keratin/keratin sulfate, dermatin/dermatan sulfate, heparin/heparan and hyaluronan. Proteoglycan in cartilage plays an important role in wound healing because the molecular structure is similar to an epidermal growth factor (EGF)-like domain. The bioactive mechanism of proteoglycan is also similar to that of EGF with regard to wound healing through promotion of epidermal growth and regeneration of tissues and blood vessels. 1 In 2015, this material was added to silver sulfadiazine to accelerate and facilitate wound healing and to promote collagen synthesis. 1 After that, they studied liver and kidney toxicity in rats receiving cream containing proteoglycan extraction from fish cartilage in 2016. This result demonstrated that the cream containing proteoglycan extracted from fish cartilage accelerated and facilitated wound healing without causing to toxic effects to the liver and kidney in rats in long term use. 2 In 2018, Bunman et al., formulated a new serum for facial use, which when applied promotes collagen synthesis and wrinkle relief. The study demonstrated that this serum has accelerated collagen synthesis and low potential skin sensitization in rat models. 3 In 2019, Bunman developed and formulated a new cream for facial use, called "bright and firm hybrid emulsion I". Bright and firm hybrid emulsion I is composed of many active ingredients such as pseudoalteromonas ferment extract, chlamydomonas extract, hamamelis virginiana (witch hazel) extract, and astaxanthin etc. to reduce wrinkles, to increase facial whitening and to promote collagen synthesis. However, this product does not have scientific findings of antioxidant activity, collagen synthesis, and stability.

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Section: The Masson's Trichrome Examinationmentioning

confidence: 99%

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