The Evaluation of Synthetic Oxygen Carriers by Perfusion of Isolated Rat Liver

Nováková, V.

doi:10.1111/j.1748-1716.1976.tb10332.x

Cited by 8 publications

(2 citation statements)

References 8 publications

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“…More over, those perfusions were carried out at normothermic, 37 °C, and hyperthermic temperatures, 43 °C. Prior studies using PFC emulsions in the perfusate for rat liver perfu sions demonstrated good hepatic function at 37 °C, but no animal survival studies were reported [6,9,14,15]. When the rat liver was preserved at 8-10 °C for 20-22 h, viability was demonstrated by transplantation into another rat which survived [8], These data do not differ significantly from previous results of dog liver perfusion using a red cell perfusate except at 43 °C.…”

Section: Discussioncontrasting

confidence: 36%

Canine Liver Isolation-Perfusion at Normo- and Hyperthermic Temperatures with Perfluorochemical Emulsion (Fluosol-43)

Skibba¹,

Sonsalla²,

Petroff³

et al. 1985

Eur Surg Res

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A perfluorocarbon emulsion, Fluosol-43, was used as a blood substitute for oxygen transport during isolation-perfusion of the dog liver at 37 and 43 °C. Preservation of hepatic functional integrity was assessed through analysis of perfusate constituents and animal survival after perfusion. Flow to the liver during perfusion was > 1 ml/min/g with one-third of total flow provided through the hepatic artery and two-thirds through the protal vein. Perfusion duration was 3 h. The pO₂ gradient across the liver indicated that oxygen was consumed during perfusion at both temperatures. The expected rise in pCO₂ and decrease in pH of the outflow perfusate is consistent with active aerobic metabolism. Perfusate chemistries lactate, pyruvate, glucose, urea, total α-amino acids, ketone bodies and SGPT demonstrated that hepatic functional integrity was maintained during perfusion. Significant differences (p < 0.05) between temperatures occurred in the perfusate levels of lactate, pyruvate, L/P ratios, glucose and total α-amino acids. Animal survival after a 3-hour perfusion was 3/4 at 37 °C, and 2/5 at 43 °C. After perfusion, SGPT levels were significantly higher in dogs subjected to perfusion at 43 °C. The success of these experiments demonstrates that perfusion of the liver with Fluosol-43 was not in itself hepatotoxic, and that Fluosol-43 may allow perfusion of the liver at 43 °C with only mild toxicity.

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Section: Discussioncontrasting

confidence: 36%

Canine Liver Isolation-Perfusion at Normo- and Hyperthermic Temperatures with Perfluorochemical Emulsion (Fluosol-43)

Skibba¹,

Sonsalla²,

Petroff³

et al. 1985

Eur Surg Res

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“…For these reasons, PFCs and PFC emulsions have been implemented in numerous clinical and research applications. For the last half of a century, substantial research has investigated the use of pure PFCs and micellar PFC suspensions (emulsions) as oxygen carriers. − Functional studies of various PFCs have found their oxygen solubilities to approximate or surpass hemoglobin, at 12–20 times that of water or physiological salt solutions at varied oxygen partial pressures. ,,,− Additionally, the dissolved oxygen content in some PFCs does not vary substantially with temperature, resulting in further enhancement in oxygen solubility due to the concomitant decreased solubility within physiological solutions . In addition to increased oxygen solubility, the effective oxygen diffusivities of PFCs are approximately 2.5- to 4-fold that of water or physiological salt solutions, such as culture medium.…”

Section: Introductionmentioning

confidence: 99%

Complementary Methods for the Determination of Dissolved Oxygen Content in Perfluorocarbon Emulsions and Other Solutions

2011

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Perfluorocarbons (PFCs) are compounds with increased oxygen solubility and effective diffusivity, making them ideal candidates for improving oxygen mass transfer in numerous biological applications. Historically, quantification of the mass transfer characteristics of these liquids has relied on the use of elaborate laboratory equipment and complicated methodologies, such as in-line gas chromatography coupled with temperature-controlled glass fritted diffusion cells. In this work, we present an alternative method for the determination of dissolved oxygen content in PFC emulsions and, by extrapolation, pure PFCs. We implemented a simple stirred oxygen consumption microchamber coupled with an enzymatic reaction for the quantitative determination of oxygen by optical density measurements. Chambers were also custom fitted with lifetime oxygen sensors to permit simultaneous measurement of internal chamber oxygen levels. Analyzing the consumption of oxygen during the enzymatic reaction via recorded oxygen depletion traces, we found a strong degree of correlation between the zero-order reaction rate and the total measured oxygen concentrations, relative to control solutions. The values obtained were in close agreement with published values in the literature, establishing the accuracy of this method. Overall, this method allows for easy, reliable, and reproducible measurements of oxygen content in aqueous solutions, including, but not limited to PFC emulsions.

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