The evaluation of the protective action of antioxidants on small intestine of rabbits experimentally injured by ischemia and reperfusion

Byrka-Owczarek, Katarzyna; Stȩplewska-Mazur, Katarzyna; Krasoń, Marcin; Bohosiewicz, Janusz; Koszutski, Tomasz; Wojtynek, Grazyna

doi:10.1016/j.jpedsurg.2004.04.009

Cited by 27 publications

(31 citation statements)

References 27 publications

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“…Protective effects of NAC may lie within its ability to up regulate antioxidant systems 6 . The efficiency of NAC protective action depends on its dosage used; literature recommends 150 mg/kg as the most efficient one 4 . Saricaoglu et al 18 observed that oxidative stress, induced by I/R injury during arthroscopic knee surgery, was attenuated in the Group of patients which received NAC infusion and anesthesia with propofol, fentanyl and vecuronium bromide, maintained with desflurane.…”

Section: Discussionmentioning

confidence: 99%

“…Immunoblotting is a specific and highly sensitive immunochemical assay for the determination of carbonyl level in a variety of biological samples 3 . However, only few studies linking ORF and protein carbonylation to intestinal disturbances are available 2,4,5 .…”

Section: Introductionmentioning

confidence: 99%

“…Administration of exogenous free radicals scavengers had been tried to reduce oxidative stress induced by I/R, and antioxidants may be particularly useful 4 . Propofol and N-Acetylcysteine (NAC), when separately administrated, exert beneficial effects on reperfusion injury 6,7 .…”

Section: Introductionmentioning

confidence: 99%

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Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: Protein carbonyl detection by immunoblotting

Azeredo¹,

Azeredo²,

Eleuthério³

et al. 2008

Acta Cir. Bras.

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Purpose:To evaluate the antioxidant effect of Propofol and N-Acetylcysteine (NAC) on intestinal ischemia/reperfusion (I/R) in rats by determining carbonyl protein level. Methods: Forty Wistar rats were randomly assigned into the following groups: Control; Sham; I/R with Propofol; I/R with Propofol and NAC; I/R with Ketamine and Xylazine. The I/R groups underwent 60 minutes of ischemia and an equal period of reperfusion. Blood samples, collected by cardiac punction, were centrifuged for plasma obtainment. Protein carbonyl level in plasma samples was determined by immunoblotting. Results: No significant difference in protein carbonyl level was found between Control and Sham groups (P>0.05). The highest reduction in protein carbonyl level (P<0.05) was obtained with the administration of Propofol and NAC (Group 4) in intestinal I/R procedure. Conclusion: The administration of Propofol and NAC showed the best antioxidant effect on oxidative stress in rats that underwent intestinal I/R procedure, suggesting a synergistic interaction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: Protein carbonyl detection by immunoblotting

Azeredo¹,

Azeredo²,

Eleuthério³

et al. 2008

Acta Cir. Bras.

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“…According to this hypothesis, the villus tips become virtually anoxic in spite of an unaltered mucosal blood flow due to shunting of oxygen between the arterioles and venules at the base of the villus. Support for this theory is provided by the observation that ischemic injury to the small bowel is most severe at the tip and is attenuated by intraluminal perfusion with oxygenated saline 26 . A variety of associations between ascorbic acid and IR injury has been described recently [27][28][29][30][31] .…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Ascorbic Acid Pretreatment in a Rat Model of Intestinal Ischemia-Reperfusion Injury: A Histomorphometric Study

Higa

Parra

Ab’Saber

et al. 2007

Clinics

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AM, Farhat C, Higa R, Capelozzi VL. Protective effects of ascorbic acid pretreatment in a rat model of intestinal ischemia-reperfusion injury: a histomorphometric study. Clinics. 2007;62(3):315-20.BACKGROUND:. Ascorbic acid has shown promise in attenuation of intestinal ischemia-reperfusion (I/R) injury. The aim of this study was to determine the protective effects of ascorbic acid on intestinal morphology during IR injury in rats. MATERIALS AND METHODS:. We examined morphological changes in the small intestine of Wistar rats after (i) 40 minutes of ischemia (I), (ii) ischemia followed by 30 min of reperfusion (IR), (iii) ischemia with ascorbic acid (IA), (iv) ischemia followed by reperfusion and ascorbic acid (IRA) and (v) in a sham group (S). We used morphometry to evaluate the amount of villous architecture, crypts, necrosis, hemorrhagic infarcts and inflammatory cells at the mesenteric and antimesenteric borders of the small intestine. RESULTS: Ascorbic acid caused a significant reduction of antimesenteric villous hemorrhagic infarction (p<0.05) of the small intestine after ischemia followed by reperfusion as well as villous necrosis reduction at both borders after ischemia (p<0.05). The lesions found in the small intestine were more prominent along the antimesenteric margin. CONCLUSIONS: Ascorbic acid pretreatment has a protective effect against the intestinal morphological lesions induced by ischemia-reperfusion injury in rats.

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“…A study using an experimental model in rabbits with vitamin C, mannitol and N-acetylcysteine has been shown that the superior mesenteric artery flow was improved by the three drugs, but only vitamin C and mannitol had direct protective action on gut mucosa at the histopathological exam of the small intestine, with significant reduction of ischemia and reperfusion injury 10 . These results were confirmed by another article that showed morphological improvement of the lesion after administration of ascorbic acid 11 .…”

Section: Discussion Discussion Discussion Discussionmentioning

confidence: 99%

Antioxidantes enterais em lesões de isquemia e reperfusão em ratos

Köhler

Delucca

Neto

2011

Rev. Col. Bras. Cir.

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Enteral antioxidants in ischemia/reperfusion injuries in rats Enteral antioxidants in ischemia/reperfusion injuries in rats Enteral antioxidants in ischemia/reperfusion injuries in rats Enteral antioxidants in ischemia/reperfusion injuries in rats Enteral antioxidants in ischemia/reperfusion injuries in rats Antioxidantes enterais em lesões de isquemia e reperfusão em ratos Antioxidantes enterais em lesões de isquemia e reperfusão em ratos Antioxidantes enterais em lesões de isquemia e reperfusão em ratos Antioxidantes enterais em lesões de isquemia e reperfusão em ratos Antioxidantes enterais em lesões de isquemia e reperfusão em ratos HUGO Methods Methods Methods Methods: Ninety adult male Wistar rats were used. An intestinal segment was isolated based on its vascular pedicle. A control biopsy was performed and the pedicle was sectioned and sutured again, ensuring a time of 60 minutes of ischemia followed by reperfusion. Sequential biopsies were performed at the end of the ischemic period and every 15 minutes during reperfusion. The treatment consisted of saline, vitamin C, vitamin E or a combination of the latter two. Quantitative and qualitative assessments of the biopsies were performed. Results Results ResultsResults Results: The groups treated with vitamin E alone or vitamin E combined with vitamin C showed a statistically significant attenuation of ischemia-reperfusion, with reduced loss of height of the villi and lower neutrophilic infiltration at the end of the study when compared to the control and vitamin C- INTRODUCTION INTRODUCTION INTRODUCTION INTRODUCTION INTRODUCTIONT he restoration of blood flow to an ischemic tissue can lead to greater harm than that originally caused by ischemia. This event is called ischemia and reperfusion (I/R) injury. The small intestine is particularly susceptible to injury from I/R 1 and its occurrence is associated with high morbidity and mortality 2 . Although the mechanisms involved are not fully elucidated, it is believed that oxidative stress mediators, such as reactive oxygen species (ROS), polymorphonuclear neutrophils (PMN) and nitric oxide (NO) play an important role 3 . Due to the involvement of ROS in I/R injury, various antioxidants have been tested, among them vitamin C (ascorbic acid) and vitamin E. Ascorbic acid is an water-soluble antioxidant with chelating and reducing properties and it is used to prevent I/R injury 4 . Vitamin E (á-tocopherol) is a nonenzymatic, lipid soluble antioxidant that acts against oxidative stress by stabilizing membrane unsaturated fatty acids. Thus, tissues treated with vitamin E have an increased capacity to reduce ROS and be protected against membrane lipid peroxidation 5 . Vitamins C and E can act synergistically because ascorbic acid is unable to reduce the peroxyl radical, but it can regenerate á-tocopherol from the tocoferoxil radical, recycling the á-tocopherol 6 . I/R can occur in a variety of clinical situations, one being the microsurgical transfer of jejunal segments. The jejunal flap was first described in 1957...

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The evaluation of the protective action of antioxidants on small intestine of rabbits experimentally injured by ischemia and reperfusion

Cited by 27 publications

References 27 publications

Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: Protein carbonyl detection by immunoblotting

Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: Protein carbonyl detection by immunoblotting

Protective Effects of Ascorbic Acid Pretreatment in a Rat Model of Intestinal Ischemia-Reperfusion Injury: A Histomorphometric Study

Antioxidantes enterais em lesões de isquemia e reperfusão em ratos

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