The Eventual Intracellular Destruction of Staphylococci by Mononuclear Cells

Shayegani, Mehdi; Kapral, Frank A.

doi:10.1099/00221287-29-4-637

Cited by 8 publications

(6 citation statements)

References 8 publications

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“…These latter studies were performed using the leucocytes from a single human donor and thus might not reflect a general phenomenon. This view might be supported by the observed variation in the behaviour of staphylococci within leucocytes derived from different rabbits (Shayegani & Kapral, 1962).…”

Section: Discussionsupporting

confidence: 60%

“…In previous publications (Kapral & Shayegani, 1959;Shayegani & Kapral, 1962) the authors reported that Staphylococcus aureus usually survived for several hours within polymorphonuclear leucocytes and monocytes of normal rabbits, but were eventually slowly destroyed within the monocytes. S. albus, however, was rapidly destroyed by these cells under the same conditions.…”

Section: Introductionmentioning

confidence: 99%

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The Immediate Fate of Staphylococci after Phagocytosis

Shayegani

Kapral

1962

Journal of General Microbiology

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SUMMARYFour methods were employed to study the immediate fate of staphylococci within leucocytes after phagocytosis. These were : ( a ) The met hod of Cohn & Morse where leucocytes and staphylococci were mixed in tubes and kept agitated. Periodically samples were removed and lightly centrifuged to sediment the leucocytes. Plate counts were made on the lysed leucocytes to measure the intracellular organisms, the supernatant to measure the extracellular population, and a non-centrifuged saniple to determine the total number of viable cocci present, (b) Concentrated suspensions of leucocytes and staphylococci were packed together by brief centrifugation to permit rapid phagocytosis without antibodies then diluted in cold Hanks solution to stop further phagocytosis. The suspension was then rapidly passed through a Servall centrifuge with the continuous flow attachment to remove excess extracellular organisms. The infected leucocytes were placed in suspension and samples removed and treated as in the previous procedure. (c) Leucocytes and staphylococci were placed in plastic chambers containing a number of small coverslips. The cells were allowed to sediment and adhere to the coverslips, then washed to remove most of the extracellular organisms. Coverslips were removed at intervals and attached leucocytes lysed to liberate intracellular cocci which were enumerated by plate counts. Companion coverslips were washed, fixed, and stained in order to count the number of leucocytes present. The extracellular staphylococcal population was estimated by making plate counts on the tissue culture medium in the chambers. ( d ) Suspensions of infected leucocytes were diluted and placed in a series of Petri dishes. The cells were permitted to settle and attach to the glass. Periodically the plates were washed and melted trypticase soy agar was added, After incubation the plate counts afforded an estimation of the viable cocci remaining within the leucocytes at each sampling.The results obtained with these procedures were in fairly good agreement with each other. It was found that Staphylococcus aureus (IS-z and Smith strains) survived in significant numbers within monocytes and polymorphonuclear leucocytes of normal rabbits for several hours. Little destruction of this organism during the first hour after phagocytosis could be demonstrated.

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Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

The Immediate Fate of Staphylococci after Phagocytosis

Shayegani

Kapral

1962

Journal of General Microbiology

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“…Administration of the antigens alone seemed to have no effect on host resistance. Since S. aureus do not multiply within the cells (13,14) and are therefore eventually eliminated, the presence of homologous antigens is needed for stimulation of cellular resistance. This finding confirms a previous report (12) describing the need for elicitation in which cell-mediated immunity was tested at the cellular level, using peritoneal macrophages from infected and elicited animals in order to study the intracellular destruction of S. aureus.…”

Section: Resultsmentioning

confidence: 99%

Nonspecific resistance to Escherichia coli in mice

Shayegani¹,

Parsons²

1975

Infect Immun

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Nonspecific cell-mediated immunity to a relatively virulent strain of Escherichia coli was studied in mice infected with Staphylococcus aureus and elicited with specific antigens. The infected and elicited mice were protected against as intraperitoneal challenge by E. coli for an observation period of 7 days, whereas normal mice, given the same number of bacteria, died within 18 to 24 h. However, the amount of time elapsing between elicitation and challenge greatly affected the rate of protection. Little or no protection was observed in mice injected with S. aureus but not elicited or in mice injected with staphylococcal antigens but not infected with staphylococci.

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“…In applying the Trudeau experience to staphylococcal infection, it is relevant that Staphylococcus aureus does not multiply in macrophages (18,19).…”

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confidence: 99%

Staphylococcidal Capability of Rabbit Peritoneal Macrophages in Relation to Infection and Elicitation: Induction and Elicitation of Activated Macrophages

Lenhart

Mudd

1972

Infect Immun

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The capability of macrophages to inactivate ingested staphylococci can be augmented when repeated infection is followed by specific elicitation with staphylococcal lysate. The increase in staphylococcidal capability with specific elicitation after infection is not dramatic but is statistically significant. The percentage of change in staphylococcidal capability after infection and specific elicitation is systematically related to the staphylococcidal capability of the populations of macrophages in the same rabbits studied prior to infection. When the capability of the initial populations of macrophages has been high, the percentage of change after infection and elicitation may be slight or even negative. When the staphylococcidal capability of the initial population of macrophages in a given rabbit has been low, there is typically a significant increase in this capability after infection and elicitation. It is shown at the cellular level that it is possible to evoke a population of activated macrophages, by a procedure which is analogous to procedures reported as useful in human practice.

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The Eventual Intracellular Destruction of Staphylococci by Mononuclear Cells

Cited by 8 publications

References 8 publications

The Immediate Fate of Staphylococci after Phagocytosis

The Immediate Fate of Staphylococci after Phagocytosis

Nonspecific resistance to Escherichia coli in mice

Staphylococcidal Capability of Rabbit Peritoneal Macrophages in Relation to Infection and Elicitation: Induction and Elicitation of Activated Macrophages

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