The evolution of emaraviruses is becoming more complex: seven segments identified in the causal agent of Rose rosette disease

Bello, Patrick L. Di; T, Ho; Tzanetakis, Ioannis E.

doi:10.1016/j.virusres.2015.08.009

Cited by 72 publications

(85 citation statements)

References 29 publications

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“…Di Bello et al . () demonstrated that RRV was transmissible with eriophyid mites and can solely cause typical RRD symptoms, fulfilling Koch's postulates for RRD/RRV. In light of this information the characteristics of transmission and resistance were revisited.…”

Section: Introductionmentioning

confidence: 62%

Transmission attributes and resistance to rose rosette virus

et al. 2017

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Rosette caused by rose rosette virus (RRV) is a devastating disease of rose in the United States. The virus was discovered in 2011 and Koch's postulates completed in 2015. Because of these recent discoveries, assumptions about the disease including movement, transmission and resistance are based on visual observations of material that may or may not have been infected by the virus. This study addresses several aspects of virus and disease dynamics. Twenty rose genotypes were screened for mite and/or virus resistance. Phyllocoptes fructiphilus, the only known vector of RRV, was able to establish, lay eggs and develop nymphs and adults in all rose genotypes. Cultivar ‘Stormy Weather’ showed resistance to the virus as assessed in both mite and cleft‐grafting transmission experiments. Mites showed a long acquisition/latent period but a rapid inoculation time for RRV. Knowledge of resistance as well as transmission attributes will assist in better management of vector and disease. The identified resistant genotype would be used in areas with high disease pressure to minimize spread, for identification of the mechanisms behind resistance or as a breeding parent to incorporate virus resistance to new cultivars. The short inoculation access period suggests that chemical control for this vector may be challenging to undertake.

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Section: Introductionmentioning

confidence: 62%

Transmission attributes and resistance to rose rosette virus

et al. 2017

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“…We identified five segments in RYRSaV using different sequencing approaches and applying the models of the Di Bello et al (2015) study which identified seven segments in the RRV genome. This possibly does not represent the complete genome of RYRSaV.…”

Section: Discussionmentioning

confidence: 99%

“…The vector phylogeny is also complex. FMV, PPSMV, PPSMV-2 and WMoV are transmitted by mites in the genus Aceria, whereas RLBV, and RRV are transmitted by mites in the genus Phyllocoptes (Caglayan et al, 2012;Di Bello et al, 2015;Kulkarni et al, 2002;McGavin et al, 2012;Tatineni et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Sequencing templates were either degenerate oligonucleotide-primed reverse transcriptase-PCR (DOP RT-PCR; Laney et al, 2011) or RT-PCR amplicons utilizing the emaravirus 5 /3 end primer PDAP213 (Di Bello et al, 2015;Supplemental reduction in the extension time to 15 s to enrich for RNAs shorter than 2 kb. Sequencing outputs were analyzed using VirFind (Ho and Tzanetakis, 2014), revealing several hundred orthologs of the previously characterized emaraviruses (data not shown).…”

Section: Virus Discoverymentioning

confidence: 99%

“…The p5 protein is most similar to EMARaV p4 than other emaravirus p5 proteins in the databases. In fact, EMARaV p4 does not share this secondary structure or sequence homology with emaravirus movement proteins, instead it likely is a ortholog of FMV p6 and RRV p6 based on secondary structural analysis (Di Bello et al, 2015;Yu et al, 2013). An alignment between RYRSaV p5, EMARaV p4 and FMV, RRV, pigeon pea sterility mosaic virus-2 (PPSMV-2) p6s showed only 15-18% identity (36-38% similarity; Altschul et al, 1997;Supplemental Table 9).…”

Section: Virus Discovery and Genome Analysismentioning

confidence: 99%

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A novel emaravirus is associated with redbud yellow ringspot disease

et al. 2016

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Yellow ringspot is the only virus-like disease reported in redbud (Cercis spp.) with symptoms including vein clearing, chlorotic ringspots and oak-leaf pattern. A putative new emaravirus was present in all trees displaying typical yellow ringspot symptoms and the name redbud yellow ringspot associated virus is proposed. The virus genome is composed of at least five RNA segments. Two coding regions were studied to determine isolate diversity with results pointing to a homogeneous virus population. Host range was evaluated using graft transmission and by testing species found in close proximity to infected trees. Mite transmission with Aculops cercidis, the predominant species found in redbud trees in the epicenter of the disease, was evaluated but was not found to be a vector of the virus. Based on this study and the accumulated knowledge on emaravirus evolution we propose that speciation is allopatric, with vectors being a major component of the process.

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