The evolution of innate immune receptors: investigating the diversity, distribution, and phylogeny of immune recognition across eukaryotes

Buckley, Katherine M.; Yoder, Jeffrey A.

doi:10.1007/s00251-021-01243-4

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…The high stability of circRNA presents a promising approach to solving stability concerns in mRNA-based vaccine development (Chaudhary et al 2021 ). An extensive repertoire of innate immune receptors has evolved to detect and respond to foreign substances (Hawash et al 2021 ; Buckley and Yoder 2022 ). Notably, nucleic acid receptors have undergone significant evolutionary adaptations to recognize the DNA or RNA of intracellular intruders, such as viruses.…”

Section: Discussionmentioning

confidence: 99%

The RNA ligation method using modified splint DNAs significantly improves the efficiency of circular RNA synthesis

Kim,

et al. 2023

Animal Cells and Systems

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Circular RNA (circRNA) is a non-coding RNA with a covalently closed loop structure and usually more stable than messenger RNA (mRNA). However, coding sequences (CDSs) following an internal ribosome entry site (IRES) in circRNAs can be translated, and this property has been recently utilized to produce proteins as novel therapeutic tools. However, it is difficult to produce large proteins from circRNAs because of the low circularization efficiency of lengthy RNAs. In this study, we report that we successfully synthesized circRNAs with the splint DNA ligation method using RNA ligase 1 and the splint DNAs, which contain complementary sequences to both ends of precursor linear RNAs. This method results in more efficient circularization than the conventional enzymatic method that does not use the splint DNAs, easily generating circRNAs that express relatively large proteins, including IgG heavy and light chains. Longer splint DNA (42 nucleotide) is more effective in circularization. Also, the use of splint DNAs with an adenine analog, 2,6-diaminopurine (DAP), increase the circularization efficiency presumably by strengthening the interaction between the splint DNAs and the precursor RNAs. The splint DNA ligation method requires 5 times more splint DNA than the precursor RNA to efficiently produce circRNAs, but our modified splint DNA ligation method can produce circRNAs using the amount of splint DNA which is equal to that of the precursor RNA. Our modified splint DNA ligation method will help develop novel therapeutic tools using circRNAs, to treat various diseases and to develop human and veterinary vaccines.

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Section: Discussionmentioning

confidence: 99%

The RNA ligation method using modified splint DNAs significantly improves the efficiency of circular RNA synthesis

Kim,

et al. 2023

Animal Cells and Systems

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“…Examples include the discovery of cyclins (1), which ultimately led to currently approved drugs targeting metastatic cancers (2), and the first descriptions of gene regulatory networks (3,4), which have become a foundational framework for understanding development (5)(6)(7). Sea urchins remain favorites for study across diverse fields including gene regulation of development (8)(9)(10), innate immunity (11)(12)(13)(14)(15)(16), biomineralization (17)(18)(19)(20)(21), nervous system development (22)(23)(24), reproduction (25)(26)(27), and developmental physiology (28)(29)(30).…”

Section: Introductionmentioning

confidence: 99%

Stable germline transgenesis using theMinosTc1/marinerelement in the sea urchin,Lytechinus pictus

Jackson,

Romero,

Kling

et al. 2024

Preprint

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Stable transgenesis is a transformative tool in model organism biology. While the sea urchin is one of the oldest animal models in cell and developmental biology, it has relied on transient manipulations of wild animals, and has lacked a strategy for stable transgenesis. Here we build on recent progress to develop a more genetically tractable sea urchin species, Lytechinus pictus, to establish a robust transgene integration method. Three commonly used transposons (Minos, Tol2, piggyBac) were tested for non-autonomous transposition, using plasmids containing a polyubiquitin promoter upstream of a H2B-mCerulean nuclear marker. Minos was the only transposable element that resulted in significant expression past metamorphosis. F0 animals were raised to sexual maturity and spawned to determine germline integration, transgene inheritance frequency, and to characterize expression patterns of the transgene in F1 progeny. The results demonstrated transgene transmission through the germline, the first example of a germline transgenic sea urchin, and indeed of any echinoderm. This milestone paves the way for the generation of diverse transgenic resources that will dramatically enhance the utility, reproducibility, and efficiency of sea urchin research.

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Contributions of marine invertebrates to our understanding of human health and disease

Hamdoun

DeTomaso

Foltz

2023

Oceans and Human Health

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The evolution of innate immune receptors: investigating the diversity, distribution, and phylogeny of immune recognition across eukaryotes

Cited by 4 publications

References 11 publications

The RNA ligation method using modified splint DNAs significantly improves the efficiency of circular RNA synthesis

The RNA ligation method using modified splint DNAs significantly improves the efficiency of circular RNA synthesis

Stable germline transgenesis using theMinosTc1/marinerelement in the sea urchin,Lytechinus pictus

Contributions of marine invertebrates to our understanding of human health and disease

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