The evolution of transplantation societies in Latin America

Identification of novel vaccine candidates for Chagas’ disease by immunization with sequential fractions of a trypomastigote cDNA expression library

“…Being aware that, within a target molecule, mouse T cell epitopes will certainly differ from human T cell epitopes we screened for the presence of both human and mouse CTL epitopes in all T. cruzi proteins and ORFs retrieved from the protective non-redundant ELI Tc-2D dataset (cloned in the sense orientation; n = 22). We restricted our analysis to the HLA-A2 human allele owing to its high frequency of expression in individuals living in Chagas' disease endemic areas of Latin America [37] and to the mouse MHC class I gene product H-2K k present in the mouse strain used in this work. Results indicated that 21 out 22 proteins and ORFs presented one or more peptides that bind with strong or intermediate affinities (i.e.…”