The Evolution of Vascular Tissue Engineering and Current State of the Art

Peck, Marissa; Gebhart, David; Dusserre, Nathalie; McAllister, Todd; L’Heureux, Nicolas

doi:10.1159/000331406

Cited by 158 publications

(128 citation statements)

References 172 publications

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“…44 These grafts demonstrated physiologic and mechanical functions comparable to native human vessels and are being considered for clinical application. 45 MSCs originate from the mesenchyme, the embryonic connective tissue that is derived from the mesoderm. Their ability to differentiate into multiple cell lineages, coupled with their…”

Section: Sheet-based Techniquesmentioning

confidence: 99%

Vessel Bioengineering

Tara

Rocco

Hibino

et al. 2014

Circ J

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The development of vascular bioengineering has led to a variety of novel treatment strategies for patients with cardiovascular disease. Notably, combining biodegradable scaffolds with autologous cell seeding to create tissue-engineered vascular grafts (TEVG) allows for in situ formation of organized neovascular tissue and we have demonstrated the clinical viability of this technique in patients with congenital heart defects. The role of the scaffold is to provide a temporary 3-dimensional structure for cells, but applying TEVG strategy to the arterial system requires scaffolds that can also endure arterial pressure. Both biodegradable synthetic polymers and extracellular matrixbased natural materials can be used to generate arterial scaffolds that satisfy these requirements. Furthermore, the role of specific cell types in tissue remodeling is crucial and as a result many different cell sources, from matured somatic cells to stem cells, are now used in a variety of arterial TEVG techniques. However, despite great progress in the field over the past decade, clinical effectiveness of small-diameter arterial TEVG (<6 mm) has remained elusive. To achieve successful translation of this complex multidisciplinary technology to the clinic, active participation of biologists, engineers, and clinicians is required. (Circ J 2014; 78: 12 -19)

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Section: Sheet-based Techniquesmentioning

confidence: 99%

Vessel Bioengineering

Tara

Rocco

Hibino

et al. 2014

Circ J

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“…1 Various approaches have been used to fabricate TEVG, [3][4][5][6][7][8][9] recently reviewed. 3 Most notably in terms of clinical promise, TEVGs derived from rolling a fibroblastproduced ''cell sheet'' into a tube 5,7,8,10 and from smooth muscle cells (SMCs) seeded onto a degradable synthetic polymer tube 4,6,9 have resulted in successful implantation. In contrast to these approaches, our approach utilizes the biopolymer fibrin (in hydrogel form) as the starting scaffold, 11 which eliminates risk of any adverse host reaction to residual synthetic material.…”

Section: Introductionmentioning

confidence: 99%

Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery

Syedain

Meier

Lahti

et al. 2014

Tissue Engineering Part A

129

108

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The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential of these unique arterial allografts. The 4-mm-diameter, 2-3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000 mmHg and the same compliance as the ovine femoral artery. Grafts were implanted interpositionally in the femoral artery of six sheep (n = 9), with contralateral sham controls (n = 3). At 8 weeks (n = 5) and 24 weeks (n = 4), all grafts were patent and showed no evidence of dilatation or mineralization. Mid-graft lumen diameter was unchanged. Extensive recellularization occurred, with most cells expressing aSMA. Endothelialization was complete by 24 weeks with elastin deposition evident. These completely biological grafts possessed circumferential alignment/mechanical anisotropy characteristic of native arteries and were cultured only 5 weeks prior to decellularization and storage as ''off-the-shelf'' grafts.

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“…In cases when anatomical difficulties hinder the preoperative study, three-dimensional printing offers the possibility of better and more personalized support. The interaction of 3D printing technology with tissue engineering is expected to be essential in the development of artificial vessels [6].…”

Section: Resultsmentioning

confidence: 99%

3D Printing of Abdominal Aortic Aneurysm

Bangeas¹,

Voulalas²,

Papadopoulos³

et al. 2015

MOJS

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hospital with upper abdominal pain and pulsate abdominal mass. On ultrasound examination an aortic aneurysm diameter (3,5cm) was found. We performed a 64-slice computed tomography (CT) of the patient's chest and abdomen visualizing the aneurysm that measured 3.5 cm in diameter.The patient was monitored and we decided to build a threedimensional (3-D) model for better preoperative planning that could be helpful for the future decision making process. We used the © Google Sketch up software. 2D computed tomography (CT) images ( Figure 1) were incorporated and reconstituted by the design program. By this way a fully rotated virtual 3D model was created ( Figure 2).Physical models of the resulting virtual 3-D surface models were created by a 3-D printer ©Makerbot with PLA (thermoplastic) filament Three-dimensional model was printed by thermoplastic material (Figures 3&4).Preoperative model demonstrated the exact anatomy of the aortic root. The replica allowed us to better understand entity by increasing our spatial perception. This is the first report of the use of 3D printing technology in patients with abdominal aortic aneurysm with or without complex anatomy. Future studies including more patients are expected to show improvement in preoperative planning and the use of computational fluid dynamics studies could enlighten and predict the process of the aneurysmal degeneration. For now, we successfully used this technology in complex cases for preoperative planning, simulation of procedures, intraoperative orientation, and postoperative evaluation of the outcome. Total fabrication time was 2h and 18min.Our experience in using rapid prototyping is small. However, we showed that 3d printing aorta models, is feasible to be created in order to be used in measurements and surgical planning of aortic aneurysm repair. Device testing and instrument development are alternative options. Although there are limitations, including small number of published studies and the cost of 3D printers. Main disadvantage of the method is the cost of commercial 3D printers. Since the technology is widely used to everyday practice, cost declines continually.

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The Evolution of Vascular Tissue Engineering and Current State of the Art

Cited by 158 publications

References 172 publications

Vessel Bioengineering

Vessel Bioengineering

Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery

3D Printing of Abdominal Aortic Aneurysm

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