The evolutionary history of Drosophila buzzatii XI. A new method for cytogenetic localization based on asynapsis of polytene chromosomes in interspecific hybrids of Drosophila

Naveira, Horacio; Plá, Carles; Fontdevila, Antonio

doi:10.1007/bf00057693

Cited by 17 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Crosses between D. buzzatii males and D. koepferae females result in F 1 sterile males and fertile females ( Marin et al 1993 ), even though a few cases of partial fertility with atrophy in one of the ovaries is also observed ( Marin and Fontdevila 1998 ). Hybrid females can be backcrossed with D. buzzatii males ( Naveira et al 1986 ; Marin and Fontdevila 1998 ). In this system, we can only obtain hybrids from crosses between D. koepferae females and D. buzzatii males, the reciprocal cross does not produce offspring ( Marin et al 1993 ).…”

Section: Introductionmentioning

confidence: 99%

High Stability of the Epigenome inDrosophilaInterspecific Hybrids

Bodelón

Fablet

Veber

et al. 2022

Genome Biology and Evolution

View full text Add to dashboard Cite

Interspecific hybridization is often seen as a genomic stress that may lead to new gene expression patterns and deregulation of transposable elements (TEs). The understanding of expression changes in hybrids compared to parental species is essential to disentangle their putative role in speciation processes. However, to date we ignore the detailed mechanisms involved in genomic deregulation in hybrids. We studied the ovarian transcriptome and epigenome of the Drosophila buzzatii and Drosophila koepferae species together with their F1 hybrid females. We found a trend towards underexpression of genes and TE families in hybrids. The epigenome in hybrids was highly similar to the parental epigenomes and showed intermediate histone enrichments between parental species in most cases. Differential gene expression in hybrids was often associated only with changes in H3K4me3 enrichments, while differential TE family expression in hybrids may be associated with changes in H3K4me3, H3K9me3, or H3K27me3 enrichments. We identified specific genes and TE families, which their differential expression in comparison to the parental species was explained by their differential chromatin mark combination enrichment. Finally, cis-trans compensatory regulation could also contribute in some way to the hybrid deregulation. This work provides the first study of histone content in Drosophila interspecific hybrids and their effect on gene and TE expression deregulation.

show abstract

Section: Introductionmentioning

confidence: 99%

High Stability of the Epigenome inDrosophilaInterspecific Hybrids

Bodelón

Fablet

Veber

et al. 2022

Genome Biology and Evolution

View full text Add to dashboard Cite

show abstract

“…The level of synapsis in interspecific hybrids is reflective of the degree of compatibility between the parental species involved. In fact, Naveira et al (1986) devised a new method for mapping gene differences between species through comparison of pairing patterns in two Drosophila species and their hybrids. Poor synapsis is reflective of remote relatedness between the parental species.…”

Section: Discussionmentioning

confidence: 99%

The Drosophila bipectinata species complex: phylogenetic relationship among different members based on chromosomal variations

Banerjee

Singh

2017

J Genet

View full text Add to dashboard Cite

Making interspecific hybridizations, where possible remains an unparalleled option for studying the intricacies of speciation. In the Drosophila bipectinata species complex comprising of four species, namely D. bipectinata, D. parabipectinata, D. malerkotliana and D. pseudoananassae, interspecific hybrids can be obtained in the laboratory, thus bequeathing an ideal opportunity for studying speciation and phylogeny. With the view of investigating the degree of divergence between each species pair, we planned to study the polytene chromosomes of the F hybrids, as it would mirror the level of compatibility between the genomes of the parental species. Two sets of crosses were made, one involving homozygous strains of all four species from India and the other including homozygous strains from different places across the globe. Polytene chromosomes of F larvae from both sets of crosses had similar configurations. In F larvae from crosses involving D. bipectinata, D. parabipectinata and D. malerkotliana, complex configurations (depicting overlapping inversions) could be detected in different arms. However, they were fairly synapsed, indicating that the differences are only at the level of gene arrangements. The polytene chromosomes of larvae obtained by crossing D. pseudoananassae with the other three species were very thin with gross asynapsis in all the arms, demonstrating that the genome of D. pseudoananassae is widely diverged from rest of the species. The overlapping inversions (reflected in complex configuration), are inferred in the light of earlier chromosomal studies performed in this complex.

show abstract

“…(1) Five strains of D. buzzatii San Luis (buzSL): buzSL-5 and buzSL-6 (Naveira & Fontdevila, 1991), founded by single wild-inseminated females collected at Serra San Luis (Argentina) and maintained by mass culturing; buzSL-101, with a white (w) mutant fixed in homozygous condition in a genetic background of buzzatii San Luis (Naveira & Fontdevila, 1991); buzSL-In(4)s, fixed for 4s, a natural population inversion of chromosome 4 (Ruiz & Fontdevila, 1981); and buzSLIn(5)1, fixed for an inversion of chromosome 5 produced in the laboratory (Naveira & Fontdevila, 1985). (2) ] is maintained by individual crosses of hybrid females (wild-type, red eyes) with buzSL-101 males (white eyes), and selection of the adult offspring from those females that, according to the analysis of polytene chromosomes in third instar larvae (Naveira et al, 1986), still contained the hmi-1-w + gametic association.…”

Section: Methodsmentioning

confidence: 99%

A system of complementary genes in hybrids between Drosophila koepferae and D. buzzatii: A Markov chain model allows to make inferences about their number and relationships.

Carvajal

2001

Genes Genet. Syst.

View full text Add to dashboard Cite

In backcrosses between D. koepferae and D. buzzatii, the disruption of a system of species-specific complementary factors brings about hybrid male inviability. This system consists of a lethal factor, hmi-1, linked to the X chromosome of D. koepferae, and several conspecific autosomal suppressors. However, hmi-1 hybrid males can also be rescued by factors present in some strains of D. buzzatii. The present work aims to estimate the number of hmi-1 suppressors in one of these strains by means of Markov chains. The obtained results allow discarding models with one or more chromosomes having independent suppressor effect. On the other hand, models having n chromosomes that interact in groups of r, being 1 < r ≤ n, to produce rescue effect, provide good approximations to the observed results. The best fit to the data is obtained with four or five chromosomes with suppressor effect, interacting epistatically in groups of three to rescue the viability of hmi-1 males.

show abstract

The evolutionary history of Drosophila buzzatii XI. A new method for cytogenetic localization based on asynapsis of polytene chromosomes in interspecific hybrids of Drosophila

Cited by 17 publications

References 34 publications

High Stability of the Epigenome inDrosophilaInterspecific Hybrids

High Stability of the Epigenome inDrosophilaInterspecific Hybrids

The Drosophila bipectinata species complex: phylogenetic relationship among different members based on chromosomal variations

A system of complementary genes in hybrids between Drosophila koepferae and D. buzzatii: A Markov chain model allows to make inferences about their number and relationships.

Contact Info

Product

Resources

About