The evolutionary puzzle of Escherichia coli ST131

Pitout, Johann; Finn, Thomas J.

doi:10.1016/j.meegid.2020.104265

Cited by 77 publications

(80 citation statements)

References 108 publications

(144 reference statements)

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“…The incidence rates and prevalence of ST131 increased over the time period, mostly caused by an influx of subclades C2 with bla CTX-M-15 and C1-M27 with bla CTX-M-27 . Such results reinforce the possible role of CTX-M enzymes in the evolutionary success of ST131 (10). The presence of bla CTX-M-14 among C1-nonM27 isolates did not provide a beneficial advantage to this subclade.…”

Section: Discussionsupporting

confidence: 65%

“…The abrupt global expansion of ST131 during the 2000s is unrivaled among human bacteria and is a real-world model for the evolution of antimicrobial-resistant high-risk clones (10). This study describes the clinical features, incidence rates, genomic characteristics, and changes in population structure of ST131 clades causing bloodstream infections in a large centralized region of Canada over an 11-year period (2006-2016).…”

Section: Discussionmentioning

confidence: 99%

“…Clade B emerged from clade A in the early 1900s and most likely occurred in North America (10,30). Members of clade B are antimicrobial susceptible, and several intermediate subclades have been identified (29).…”

Section: Discussionmentioning

confidence: 99%

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Trends in Population Dynamics ofEscherichia coliSequence Type 131, Calgary, Alberta, Canada, 2006–20161

Peirano¹,

Lynch²,

Matsumara³

et al. 2020

Emerg. Infect. Dis.

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E scherichia coli sequence type (ST) 131 is the quintessential example of a successful, global, antimicrobial-resistant, high-risk clone among human bacteria (1,2). Currently, ST131 is the most common global extraintestinal pathogenic E. coli (ExPEC) clone; up to 30% of all ExPEC, 60%-90% of fluoroquinolone-resistant ExPEC, and 40%-80% of ExPEC with extended-spectrum β-lactamases [ESBLs] belong to ST131 (3,4). Population genetics indicate that ST131 consists of different clades (5): clade A contains serotype O16:H5 and fimH41, clade B contains mostly serotype O25b:H4 and fimH22, and clade C contains serotype O25b:H4 and fimH30. Clade C is divided into 2 subclades: C1/H30R (associated with fluoroquinolone resistance) and C2/H30Rx (associated with fluoroquinolone resistance and bla CTX-M-15). A novel ST131 C1 subclade, known as C1-M27 with bla CTX-M-27 , was reported in Japan (6). ST131 is the most dominant and most antimicrobial-resistant among E. coli causing bloodstream infections in Calgary, Alberta, Canada, infecting mostly the elderly in long-term care centers (7). Previous molecular epidemiology studies from the same region showed that ST131 was relatively rare among ESBL-producing and fluoroquinoloneresistant E. coli during the early 2000s but showed a major increase toward the end of the 2000s (8,9). However, limited information is available regarding the changes in population dynamics of ST131 clades over extended periods, especially among nonbiased E. coli isolates in large, well-defined, geographic regions. To address this issue, we conducted a retrospective cohort study that characterized ST131 clades responsible for bloodstream infections in Calgary over an 11-year period (2006-2016). Investigating trends of ST131 clades over long periods by using a populationbased surveillance approach will aid in clarifying the evolution of this clone and help with designing superior prevention strategies (3,10).

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

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Trends in Population Dynamics ofEscherichia coliSequence Type 131, Calgary, Alberta, Canada, 2006–20161

Peirano¹,

Lynch²,

Matsumara³

et al. 2020

Emerg. Infect. Dis.

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“…Among the five ST131 E. coli isolates, four were genetically close (Figure 1). However, these isolates did not share the same resistome indicating that ST131 is widely distributed independent of the ESBL content as previously observed (Pitout and Finn, 2020). Moreover, the two closest ST131 (S65 and S17-2) possessing the bla CTX−M−27 ESBL gene were recovered one-year apart indicating the persistence or continuing contamination by this clone.…”

Section: Clonal Relationshipmentioning

confidence: 55%

Occurrence and Diversity of CTX-M-Producing Escherichia coli From the Seine River

Girlich

Bonnin

2020

Front. Microbiol.

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CTX-M-producing Escherichia coli are spreading since 1999 both in clinical and in community settings. Environmental samples such as rivers have also been pointed out as being vectors for ESBL producers. In this report, we have investigated the presence and the diversity of CTX-M-producing E. coli isolates in two samplings of the Seine River (next to Notre Dame), Paris France, performed in June 2016 and 2017. The total number of bacteria growing on the selective ChromID ESBL agar was 3.1 × 105 cfu/L (23.8% of all growing bacteria) in 2016, whereas it was 100-fold lower in 2017 (3 × 103 cfu/L; 8.3% of all growing bacteria). However, among them, the prevalence of ESBL-producing E. coli increased from <0.1 to 1.1% in one-year. ESBLs were exclusively of the CTX-M-type: CTX-M-1 (n = 5), CTX-M-15 (n = 7), CTX-M-14 (n = 1), and CTX-M-27 (n = 2). The isolates belonged to several multi locus sequence types, and a wide diversity of incompatibility groups of plasmids were identified in those E. coli isolates. The occurrence and diversity of E. coli isolates belonging to many clones and producing many CTX-M-variants have been identified in our study. The presence of these bacteria in rivers that are open again for recreational usage (swimming) is worrying as it may contribute to further dissemination of ESBL producers in the community.

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“…The group of aminopenicillins and inhibitor-protected penicillins cannot be used for empirical therapy of UT infections, with the exception of certain cases (for example, the proven gram-positive nature of the disease), due to the high resistance of E. coli throughout the world (on the territory of the Russian Federation it is more than 20%). In addition, the use of these drugs increases the risk of developing microbiological collateral damage (Gupta et al 2011;Grabe et al 2015;Sidorenko et al 2016;Zaycev et al 2019;Bonkat et al 2019;Pitout and Finn 2020).…”

Section: Resultsmentioning

confidence: 99%

Rational antimicrobial chemotherapy: assessment of the level of basic knowledge of general practitioners. Final results of the KANT project

Bontsevich¹,

Adonina²,

Gavrilova³

et al. 2020

RRP

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Introduction: The irrational use of medicines leads to a decrease in the quality of care, an increase in treatment costs and side effects. In the case of antibacterial drugs, in addition to all the above-mentioned consequences, their improper use can lead to an aggravation of the existing and quite challenging problem of our time – the growth of antibiotic resistance among pathogenic microorganisms. The aim of the study: to determine the level of basic knowledge of medical specialists in the field of a rational use of antimicrobial drugs (AMD). Materials and methods: The study was based on an analysis of an anonymous multicenter survey in the framework of the KANT project (the full name of the project is “Physicians’ (Students’) Knowledge of Antimicrobials Usage”). It was conducted in 2018–2019 in 10 major centers of Russia. Results and iscussion: According to the results of the study, the respondents showed a low level of knowledge of the rational use of antibacterial drugs. The best results are obtained for questions No.1 (time interval for evaluating the effectiveness of the initial antimicrobial therapy (AMT)), No.2 (rationality and period of AMD change with a positive clinical effect), and No.9 (determining the mode of using the proposed drugs), whereas the worst results were obtained for questions No.3 (determining irrational combinations of AMD), No.4 (determining a situation requiring a long course of AMT), and No.7 (choosing auxiliary drugs for bacterial respiratory infections). Conclusion: The results obtained in the study indicate the need for additional educational activities among health professionals.

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The evolutionary puzzle of Escherichia coli ST131

Cited by 77 publications

References 108 publications

Trends in Population Dynamics ofEscherichia coliSequence Type 131, Calgary, Alberta, Canada, 2006–20161

Trends in Population Dynamics ofEscherichia coliSequence Type 131, Calgary, Alberta, Canada, 2006–20161

Occurrence and Diversity of CTX-M-Producing Escherichia coli From the Seine River

Rational antimicrobial chemotherapy: assessment of the level of basic knowledge of general practitioners. Final results of the KANT project

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