The evolving role of ankyrin-B in cardiovascular disease

Koenig, Sara N.; Mohler, Peter J.

doi:10.1016/j.hrthm.2017.07.032

Cited by 37 publications

(32 citation statements)

References 73 publications

(78 reference statements)

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“…5). Similarly to THY1, ANK2 is not typically expressed in oviductal epithelial cells, since it is primarily localized in cardiomyocytes, with these gene's loss of function resultingin arrythmia [26].…”

Section: Discussionmentioning

confidence: 99%

The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivation

Stefańska

Kocherova

Knap

et al. 2019

Medical Journal of Cell Biology

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The oviduct is a part of female reproductive tract that is essential for successful fertilization and early embryo development. It is lined with epithelium consisting of two types of cells: ciliated and secretory. The primary function of ciliated oviductal epithelial cells (OECs) is to support the transport of gametes and embryos through the ovary, whereas secretory OECs produce components of the oviductal fluid. Undoubtedly, the oviductal epithelium plays a major part in the early aspects of pregnancy development, from providing an optimal environment for gametes and embryos to supporting fertilization. Therefore, our aim was to gain a better insight into the genetic changes underlying function of these cells. We have harvested OECs from crossbred gilts (n=45), at the age of about nine months and which displayed two regular estrous cycles, and established long-term primary culture of porcine OECs. Microarray analysis was utilized to determine differentially expressed genes during day 1, 7, 15 and 30 of cultivation, with our results re-vealing54 differentially expressed genes belonging to three ontology groups: "maintenance of location", "maintenance of protein location" and "maintenance of protein location in cell". Since the biochemistry and morphology of epithelial cells may change during long term cultivation, we conclude that our results are a reflection of these changes and help to shed a light on porcine OECs properties in in vitro environment.Running title: Maintenance of cellular protein location in porcine epithelial oviductal cells

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Section: Discussionmentioning

confidence: 99%

The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivation

Stefańska

Kocherova

Knap

et al. 2019

Medical Journal of Cell Biology

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“…The regulation of T-type calcium channels by cytoskeletal elements is likely to extend beyond the nervous system. For example, ankyrin B is highly expressed in cardiac myocytes where it regulates excitation-contraction coupling in concert with other signaling molecules [ 37 ]. Myocytes also express Cav3.1 and Cav3.2 T-type calcium channels, especially during early post-natal development [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B

et al. 2018

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This study describes the functional interaction between the Cav3.1 and Cav3.2 T-type calcium channels and cytoskeletal spectrin (α/β) and ankyrin B proteins. The interactions were identified utilizing a proteomic approach to identify proteins that interact with a conserved negatively charged cytosolic region present in the carboxy-terminus of T-type calcium channels. Deletion of this stretch of amino acids decreased binding of Cav3.1 and Cav3.2 calcium channels to spectrin (α/β) and ankyrin B and notably also reduced T-type whole cell current densities in expression systems. Furthermore, fluorescence recovery after photobleaching analysis of mutant channels lacking the proximal C-terminus region revealed reduced recovery of both Cav3.1 and Cav3.2 mutant channels in hippocampal neurons. Knockdown of spectrin α and ankyrin B decreased the density of endogenous Cav3.2 in hippocampal neurons. These findings reveal spectrin (α/β) / ankyrin B cytoskeletal and signaling proteins as key regulators of T-type calcium channels expressed in the nervous system.Electronic supplementary materialThe online version of this article (10.1186/s13041-018-0368-5) contains supplementary material, which is available to authorized users.

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“…The analysis aimed to validate the partners whose transcripts abundances oscillate in phase with that of Ank2 among biological replicas to satisfy the "transcriptomic stoichiometry" [27]. Figure 8 presents the significant expression coordination between the expression levels of Ank2, a major player in the cardiac physiology, with its potential binding partners [26] in the four heart chambers. While no statistically significant antagonism or independence was found, the percentages of significant synergisms were very different: 12% in the left atrium, 40% in the right atrium, 100% in the left ventricle and 72% in the right ventricle.…”

Section: Expression Coordination Of Ankyrin B With Its Potential Bindmentioning

confidence: 99%

“…We explored a number of functional pathways, addressing the differences in expression level, control and coordination between the two atria, the two ventricles, and atrium-ventricle on the same side and discuss why these differences are important for the heart function. A particular attention was given to the expression coordination of Ank2 (encoding Ankyrin-B), a major player in cardiac physiology, with its potential binding partners [26] in each heart chamber. We studied also the expression coordination of the genes encoding ion channels and transporters (ICT) in each chamber as well as the synchronous expressions of the ICT genes between chambers.…”

Section: Introductionmentioning

confidence: 99%

Up-Down and Left-Right by the Heart Transcriptome

Iacobaş¹,

Amuzescu²,

Iacobaş³

2019

Preprint

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Myocardium transcriptomes of mouse left and right atria and ventricles were profiled separately to identify the differences that might be responsible for the distinct functional roles of the four heart chambers. In total, 16,886 distinct unigenes have been quantified in all 16 samples collected from four adult male mice from the same litter. 15.76% of the quantified genes on the left and 16.5% on the right exhibited differential expression between the corresponding atrium and ventricle of the same side, while 5.8% in atria and 1.2% in ventricles were differently expressed between the left and the right. Beyond the differentially expressed genes, the study revealed distinct expression control and coordination of ion channels and genes within the cardiac muscle contraction, oxidative phosphorylation, glycolysis/glucogenesis, calcium and adrenergic signaling pathways. Interestingly, while expression of Ank2 (encoding ankyrin-B) oscillates in phase with all its binding partners in the left ventricle, the percentage of synergistically expressed partners of Ank2 is 15% and 37% in the left and right atria and 74% in the right ventricle. The analysis revealed also the high interventricular synchrony of the expression of ion channels.

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The evolving role of ankyrin-B in cardiovascular disease

Cited by 37 publications

References 73 publications

The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivation

The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivation

T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B

Up-Down and Left-Right by the Heart Transcriptome

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