The examination of aerogel composite artificial bone substitutes in animal models

Szabó, Béla Attila; Kiss, László Péter; Manó, Sándor; Jónás, Zoltán; Lázár, István; Fábián, István; Dezső, Balázs; Csernátony, Zoltán

doi:10.17489/biohun/2013/2/07

Cited by 6 publications

(10 citation statements)

References 10 publications

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“…Aerogels are 3D porous nanostructured materials with exceptional physical properties, such as extremely low densities (0.003–0.5 g cm –3 ), high specific surface areas (100–1200 m 2 g –1 ), high porosities (80–99.9%), and tunable surface and bulk chemistries. , Thanks to these promising features, they can be considered interesting, supportive templates for bone formation. However, the lack of micron-sized porosity and poor mechanical strength combined with poor machinability or shaping to a sophisticated construct are still important bottlenecks, which should be mitigated to achieve a real breakthrough in aerogel-mediated BTE. , Therefore, further research on aerogels is required to (1) develop robust processing techniques to confer controlled macroporosity to aerogel for host cell colonization, (2) improve the aerogel synthesis method to achieve a mechanically strong scaffold that can temporarily substitute natural tissues without compromising the biocompatibility of the materials, and (3) allow the incorporation of bioactive compounds (e.g., growth factor) in high yields to impart bioactivity to respective scaffolds and promote new tissue growth. − …”

Section: Introductionmentioning

confidence: 99%

Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration

Maleki

Shahbazi

Montes

et al. 2019

ACS Appl. Mater. Interfaces

129

152

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Due to the synergic feature of individual components in hybrid (nano)biomaterials, their application in regenerative medicine has drawn significant attention. Aiming to address all the current challenges of aerogel as a potent scaffold in bone tissue engineering application, we adopted a novel synthesis approach to synergistically improve the pore size regime and mechanical strength in the aerogel. The three-dimensional aerogel scaffold in this study has been synthesized through a versatile one-pot aqueous-based sol−gel hybridization/assembly of organosilane (tetraethyl orthosilicate) and silk fibroin (SF) biopolymer, followed by unidirectional freeze-casting of the as-prepared hybrid gel and supercritical drying. The developed ultralight silica-SF aerogel hybrids demonstrated a hierarchically organized porous structure with interesting honeycomb-shaped micromorphology and microstructural alignment (anisotropy) in varied length scales. The average macropore size of the hybrid aerogel lied in ∼0.5−18 μm and was systematically controlled with freeze-casting conditions. Together with high porosity (91−94%), high Young's modulus (∼4−7 MPa, >3 order of magnitude improvement compared to their pristine aerogel counterparts), and bone-type anisotropy in the mechanical compressive behavior, the silica-SF hybrid aerogel of this study acted as a very competent scaffold for bone tissue formation. The results of in vitro assessments revealed that the silica-SF aerogel is not only cytocompatible and nonhemolytic but also acted as an open porous microenvironment to trigger osteoblast cell attachment, growth, and proliferation on its surface within 14 days of incubation. Moreover, to support the in vitro results, in vivo bone formation within the aerogel implant in the bone defect site was studied. The X-ray radiology and microcomputed tomography analyses confirmed that a significant new bone tissue density formed in the defect site within 25 days of implantation. Also, in vivo toxicology studies showed a zero-toxic impact of the aerogel implant on the blood biochemical and hematological parameters. Finally, the study clearly shows the potential of aerogel as a bioactive and osteoconductive open porous cellular matrix for a successful osseointegration process.

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Section: Introductionmentioning

confidence: 99%

Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration

Maleki

Shahbazi

Montes

et al. 2019

ACS Appl. Mater. Interfaces

129

152

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“…Some aerogel formulations have found an applicability for being used as biocompatible bone graft [56] and dental substitutes [49].…”

mentioning

confidence: 99%

Synthesis and biomedical applications of aerogels: Possibilities and challenges

Maleki

Durães

García‐González

et al. 2016

Advances in Colloid and Interface Science

306

192

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“…In vivo, hydroxyapatite layer formation occurs only in the active cellular remodelling phase of the bone tissues, and no hydroxyapatite is formed spontaneously just by contact with the blood serum. Furthermore, the same types of aerogel-based ceramic materials were studied in vivo earlier in a series of animal experiments and showed excellent bioactivities in rats [56,57]. From the results, we must conclude that the better we approach the physiological conditions, the less reliable the in vitro tests are, and it is not recommended to rank or sort off the artificial bone substitute materials only on the basis of their in vitro performances.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Chemical Composition of Simulated Body Fluids on Aerogel-Based Bioactive Composites

2017

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Replacement of damaged or missing bone tissue is a serious problem in orthopedic surgery. Although various artificial materials are available, none of them fulfil the requirements completely. In this study, new bone substitute materials, silica aerogel-based β-tricalcium phosphate, and hydroxyapatite composite ceramics, along with a control sample were synthesized and tested. Porosities and pore size distribution curves were determined by nitrogen gas adsorption/desorption porosimetry, and surface morphology changes were studied by scanning electron microscopy. Bioactivities were tested in vitro by soaking the samples in simulated body fluids (SBF). Three new advanced SBFs containing eight essential amino acids and bovine serum albumin were developed, extending the complexity of the original simulated body fluid in order to approximate the human blood plasma's composition more accurately. Each sample was treated with SBF1-SBF4 for two weeks. According to our results, it seems to be necessary to re-evaluate hydroxyapatite deposition as proof of bioactivity of artificial bone substitutes when synthetic body fluids analogous in their composition to human blood plasma are used in studies.

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The examination of aerogel composite artificial bone substitutes in animal models

Cited by 6 publications

References 10 publications

Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration

Mechanically Strong Silica-Silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration

Synthesis and biomedical applications of aerogels: Possibilities and challenges

Effect of the Chemical Composition of Simulated Body Fluids on Aerogel-Based Bioactive Composites

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