The Exchangeable Apolipoprotein Nplp2 Sustains Lipid Flow and Heat Acclimation in Drosophila

Rommelaere, Samuel; Boquete, Jean-Philippe; Piton, Jérémie; Kondo, Shu; Lemaître, Bruno

doi:10.1016/j.celrep.2019.03.074

Cited by 19 publications

(16 citation statements)

References 52 publications

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“…The PL‐vir1 cluster contains 4.5% of the total hemocytes and expresses the same strong markers as PL‐Rel, including Rel, Jra, and Puc (Fig D, Dataset EV2). Compared to PL‐Rel, however, the marker of viral infection Vir1 (Dostert et al , ), the protein Pastrel involved in resistance to virus infection (Magwire et al , ; Martins et al , ), the predicted peptidase Ance‐5, and the apolipoprotein Nplp2 (Rommelaere et al , ) are all up‐regulated. PL‐vir1 also presents the same GO terms as PL‐Rel, with respect to the defense response to bacterium and to the Toll signaling pathway (Fig C).…”

Section: Resultsmentioning

confidence: 99%

Temporal specificity and heterogeneity of Drosophila immune cells

et al. 2020

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Immune cells provide defense against non‐self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity of Drosophila immune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single‐cell RNA sequencing, we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct processes, e.g., proliferation, phagocytosis, metabolic homeostasis, and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation, which triggers the differentiation of a novel hemocyte type, the lamellocyte. This first molecular atlas of hemocytes provides insights and paves the way to study the biology of the Drosophila immune cells in physiological and pathological conditions.

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Section: Resultsmentioning

confidence: 99%

Temporal specificity and heterogeneity of Drosophila immune cells

et al. 2020

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“…2b). We scored highly enriched genes in the potential intermediate subclusters and noticed that expression of Nplp2, a novel apolipoprotein 38 , peaks at PH4, GST-rich, PM1, and CC1 and is lower in other subclusters (Fig. 2b).…”

Section: Resultsmentioning

confidence: 99%

Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila

et al. 2020

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The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution.

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“…Gut hormones also play key roles in metabolic adaptations and signal to a diverse set of target organs. Genetic, transcriptomic, and immunohistochemical evidence suggests that larval or adult midgut enteroendocrine cells express *AstA, *Allatostatin C (AstC), BursA, *CCHa1, *CCHa2, CNMamide (CNMa), Crustacean cardioactive peptide (CCAP), *Diuretic hormone 31 (Dh31), Ion-transport peptide (ITP), *Myoinhibitory peptide/Allatostatin B (MIP), Neuropeptide F (NPF), Neuropeptide-like precursor 2 (NPLP2, likely functioning as an apolipoprotein rather than, or in addition to, as a prepropeptide [190]), Orcokinin, *sNPF, and *Tachykinin (Tk), expressed in stereotyped combinations and anatomical regions [191][192][193][194][195][196][197]. However, without evidence of proper peptide processing and release, prepropeptide expression alone is insufficient to prove biological activity.…”

Section: Signals That Regulate the Ipcsmentioning

confidence: 99%

Metabolism and growth adaptation to environmental conditions in Drosophila

Kaburagi

Texada

Halberg

et al. 2020

Cell. Mol. Life Sci.

108

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Organisms adapt to changing environments by adjusting their development, metabolism, and behavior to improve their chances of survival and reproduction. To achieve such flexibility, organisms must be able to sense and respond to changes in external environmental conditions and their internal state. Metabolic adaptation in response to altered nutrient availability is key to maintaining energy homeostasis and sustaining developmental growth. Furthermore, environmental variables exert major influences on growth and final adult body size in animals. This developmental plasticity depends on adaptive responses to internal state and external cues that are essential for developmental processes. Genetic studies have shown that the fruit fly Drosophila, similarly to mammals, regulates its metabolism, growth, and behavior in response to the environment through several key hormones including insulin, peptides with glucagon-like function, and steroid hormones. Here we review emerging evidence showing that various environmental cues and internal conditions are sensed in different organs that, via inter-organ communication, relay information to neuroendocrine centers that control insulin and steroid signaling. This review focuses on endocrine regulation of development, metabolism, and behavior in Drosophila, highlighting recent advances in the role of the neuroendocrine system as a signaling hub that integrates environmental inputs and drives adaptive responses.

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The Exchangeable Apolipoprotein Nplp2 Sustains Lipid Flow and Heat Acclimation in Drosophila

Abstract: Highlights d We identified Nplp2, an exchangeable apolipoprotein in Drosophila d Nplp2 maximizes Lipophorin lipid loading capacity d Nplp2 controls dietary lipid extraction and optimal fat storage d Nplp2 fuels the stress response with dietary lipids and promotes heat acclimation

Cited by 19 publications

References 52 publications

Temporal specificity and heterogeneity of Drosophila immune cells

Temporal specificity and heterogeneity of Drosophila immune cells

Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila

Metabolism and growth adaptation to environmental conditions in Drosophila

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