The exclusion of human serum albumin by human dermal collagenous fibres and within human dermis.

Abstract

Preparations of dermal collagenous fibres and slices of human dermis have been equilibrated with 125I-labelled monomeric human serum albumin. The space inaccessible to the albumin in the fibres and in the dermis was determined by subtraction of the accessible space, calculated from the radioactivity of the specimen, from its total fluid. For a fibre preparation examined in detail, the fluid exclusion was independent of the concentration of either albumin or collagen. Binding of albumin to the fibres was not demonstrable. Three fibre preparations excluded albumin from 3.75 +/- 0.96, 3.55 +/- 0.67, and 2.05 +/- 0.39 g of fluid/g of collagen (+/-S.D.). Slices from three specimens of dermis excluded albumin from 1.45 +/- 0.08 g of fluid/g of insoluble solids or 1.57 +/- 0.11 g of fluid/g of collagen (+/-S.D.). Thus the exclusion of albumin by dermis was much less than expected from its content of collagenous fibres. On the basis of these data and the published composition of dermis, the concentration of albumin in the accessible interstitial space was estimated to be close to that in the plasma.

Relation Between Trans‐synovial Flow and Plasma Osmotic Pressure, With an Estimation of the Albumin Reflection Coefficient in the Rabbit Knee

“…Gel electrophoresis of sample B indicated that -10 % of the albumin was in the form of dimers (P. Knox, personal communication). Oligomer contents of 3-34% have been reported in other commercial albumin preparations (Blaabjerg, Hyltoft & Peterson, 1979;Bert, Mathieson & Pearce, 1982). J.…”

Lymphatic Transport of Proteins After Subcutaneous Administration

“…This network is thought to effectively reduce the distribution volume such that the interstitium acts in a size exclusion manner, excluding very large molecules and thereby promoting their interstitial transport. 19,27,[30][31][32] The process of macromolecular diffusion across the interstitium is likely dictated by molecular size, the presence of diffusional microdomains, and physical and electrostatic interactions with the various components of the interstitium. In this regard, Bell and co-workers suggest that steric effects predominate, 19 however, a clear picture of these complex interactions is still elusive.…”

Improved Method for Determining Partition and Diffusion Coefficients in Human Dermis

“…The total concentration of serum proteins in dermis is 11 mg/g, based on the measurements of Bert et al16 in postmortem human dermis. The concentration in the albumin accessible regions (approximately 32% of the fluid fraction)17 has been estimated to be 2.7% (w/v) 7. It is furthermore evident that albumin and other serum proteins can migrate slowly through the tissue; otherwise, they would accumulate in dermis rather than being cleared in the lymph.…”