The Exoproteome and Surfaceome of Toxigenic <i>Corynebacterium diphtheriae</i> 1737 and Its Response to Iron Restriction and Growth on Human Hemoglobin

Goring, Andrew K.; Hale, Scott; Dasika, Poojita; Chen, Yu; Clubb, Robert T.; Loo, Joseph A.

doi:10.1021/acs.jproteome.4c00443

J. Proteome Res.

2024

DOI: 10.1021/acs.jproteome.4c00443

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The Exoproteome and Surfaceome of Toxigenic Corynebacterium diphtheriae 1737 and Its Response to Iron Restriction and Growth on Human Hemoglobin

Andrew K. Goring,

Scott Hale,

Poojita Dasika

et al.

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Molecular basis of hemoglobin binding and heme removal in Corynebacterium diphtheriae

Mahoney,

Lyman,

Ford

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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To successfully mount infections, nearly all bacterial pathogens must acquire iron, a key metal cofactor that primarily resides within human hemoglobin. Corynebacterium diphtheriae causes the life-threatening respiratory disease diphtheria and captures hemoglobin for iron scavenging using the surface-displayed receptor HbpA. Here, we show using X-ray crystallography, NMR, and in situ binding measurements that C. diphtheriae selectively captures iron-loaded hemoglobin by partially ensconcing the heme molecules of its α subunits. Quantitative growth and heme release measurements are compatible with C. diphtheriae acquiring heme passively released from hemoglobin’s β subunits. We propose a model in which HbpA and heme-binding receptors collectively function on the C. diphtheriae surface to capture hemoglobin and its spontaneously released heme. Acquisition mechanisms that exploit the propensity of hemoglobin’s β subunit to release heme likely represent a common strategy used by bacterial pathogens to obtain iron during infections.

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Molecular basis of hemoglobin binding and heme removal in Corynebacterium diphtheriae

Mahoney,

Lyman,

Ford

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

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The Exoproteome and Surfaceome of Toxigenic Corynebacterium diphtheriae 1737 and Its Response to Iron Restriction and Growth on Human Hemoglobin

Cited by 1 publication

References 96 publications

Molecular basis of hemoglobin binding and heme removal in Corynebacterium diphtheriae

Molecular basis of hemoglobin binding and heme removal in Corynebacterium diphtheriae

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