The expanding world of plant J-domain proteins

Verma, Amit; Tamadaddi, Chetana; Tak, Yogesh; Lal, Silviya S.; Cole, Sierra J.; Hines, Justin K.; Sahi, Chandan

doi:10.1080/07352689.2019.1693716

Cited by 23 publications

(12 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prion phenomenon is not limited to mammals, and numerous proteins have been discovered in the baker’s yeast Saccharomyces cerevisiae that form self-templating, heritable aggregates [ 2 , 3 , 4 , 5 , 6 , 7 ]. The most studied yeast prions, [ PSI + ], [ PIN + ]/[ RNQ + ], [ URE3 ], and [ SWI + ] are the amyloid forms of the proteins Sup35, Rnq1, Ure2, and Swi1, respectively [ 8 ], and have become useful tools not only for studying yeast prion characteristics and propagation, but also understanding chaperone protein networks in both yeast and higher organisms [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Hsp40/JDP Requirements for the Propagation of Synthetic Yeast Prions

Miller

Wegrzynowicz

Cole

et al. 2022

Viruses

View full text Add to dashboard Cite

Yeast prions are protein-based transmissible elements, most of which are amyloids. The chaperone protein network in yeast is inexorably linked to the spreading of prions during cell division by fragmentation of amyloid prion aggregates. Specifically, the core “prion fragmentation machinery” includes the proteins Hsp104, Hsp70 and the Hsp40/J-domain protein (JDP) Sis1. Numerous novel amyloid-forming proteins have been created and examined in the yeast system and occasionally these amyloids are also capable of continuous Hsp104-dependent propagation in cell populations, forming synthetic prions. However, additional chaperone requirements, if any, have not been determined. Here, we report the first instances of a JDP-Hsp70 system requirement for the propagation of synthetic prions. We utilized constructs from a system of engineered prions with prion-forming domains (PrDs) consisting of a polyQ stretch interrupted by a single heterologous amino acid interspersed every fifth residue. These “polyQX” PrDs are fused to the MC domains of Sup35, creating chimeric proteins of which a subset forms synthetic prions in yeast. For four of these prions, we show that SIS1 repression causes prion loss in a manner consistent with Sis1′s known role in prion fragmentation. PolyQX prions were sensitive to Sis1 expression levels to differing degrees, congruent with the variability observed among native prions. Our results expand the scope known Sis1 functionality, demonstrating that Sis1 acts on amyloids broadly, rather than through specific protein–protein interactions with individual yeast prion-forming proteins.

show abstract

Section: Introductionmentioning

confidence: 99%

Hsp40/JDP Requirements for the Propagation of Synthetic Yeast Prions

Miller

Wegrzynowicz

Cole

et al. 2022

Viruses

View full text Add to dashboard Cite

show abstract

“…Normally, the studies on plant HSP40s are focused on stress responses. However, there are also a few reports about the regulation of plant growth by HSP40s (Verma et al., 2019). For example, AtDjC17 was reported to be important in root hair development through cell fate determination in a tissue‐specific manner (Petti et al., 2014).…”

Section: Discussionmentioning

confidence: 99%

GmDNJ1, a type‐I heat shock protein 40 (HSP40), is responsible for both Growth and heat tolerance in soybean

Wong

Cheng

et al. 2021

Plant Direct

View full text Add to dashboard Cite

Global warming poses severe threats to agricultural production, including soybean. One of the major mechanisms for organisms to combat heat stress is through heat shock proteins (HSPs) that stabilize protein structures at above‐optimum temperatures, by assisting in the folding of nascent, misfolded, or unfolded proteins. The HSP40 subgroups, or the J‐domain proteins, functions as co‐chaperones. They capture proteins that require folding or refolding and pass them on to HSP70 for processing. In this study, we have identified a type‐I HSP40 gene in soybean, GmDNJ1, with high basal expression under normal growth conditions and also highly inducible under abiotic stresses, especially heat. Gmdnj1‐knockout mutants had diminished growth in normal conditions, and when under heat stress, exhibited more severe browning, reduced chlorophyll contents, higher reactive oxygen species (ROS) contents, and higher induction of heat stress‐responsive transcription factors and ROS‐scavenging enzyme‐encoding genes. Under both normal and heat‐stress conditions, the mutant lines accumulated more aggregated proteins involved in protein catabolism, sugar metabolism, and membrane transportation, in both roots and leaves. In summary, GmDNJ1 plays crucial roles in the overall plant growth and heat tolerance in soybean, probably through the surveillance of misfolded proteins for refolding to maintain the full capacity of cellular functions.

show abstract

“…Plant response to stress involves the triggering of mechanisms to sense the stressful signal to enable an optimal growth response. As part of this response, specific transcription factors (TFs) bind to cis-acting regulatory elements (CAREs) at the upstream region of stress responsive genes (Verma et al 2016). Considering the multiple biotic and abiotic stressful factors that could affect plant survival, development and growth, a growing amount of evidence is emerging to highlight the omnipresence of a crosstalk between the diverse response pathways from different stressor signals.…”

Section: Original Paper the Dnaj Gene Family In Yerba Mate (Ilex Para...mentioning

confidence: 99%

The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis

Aguilera

Debat²,

Castrillo

et al. 2023

Rodriguésia

View full text Add to dashboard Cite

Dry leaves and twigs of yerba mate are widely infusion-consumed in southern Southamerica. Endemic and adapted to the Atlantic Forest, its extensive full-sun monoculture links to diverse biotic (pest, pathogens) and abiotic stresses (solar radiation, drought), impacting its productivity, ecology and socioeconomic niche. We focused in comprehensively characterize the DNAJ gene family in yerba mate to predict its possible roles on development and diverse stress responses to further assist crop manage. Our results suggest that yerba mate DNAJ proteins account 140 diverse members of six structural types displaying potential variable roles in protein homeostasis control. We were able to classify them into 51 distinct orthology groups, in agreement to Arabidopsis, and performed translational genomics of function, localization, expression and stress responsiveness data. Genome mapping and expression analysis indicated that yerba mate DNAJ genes differ in expression, nucleotide composition, length and exon-intron structure. Intronless or few introns genes -linked to rapid stress response- accounted 85 DNAJs. Promoters of DNAJ genes harbored a 73.2% of cis-acting regulatory elements involved in response to diverse stresses, hormones and light, simultaneously. We hypothesize that yerba mate DNAJs assist to plant survival during multiple stresses linked to current dominant agroecosystem but promote its growth under shade.

show abstract

The expanding world of plant J-domain proteins

Cited by 23 publications

References 115 publications

Hsp40/JDP Requirements for the Propagation of Synthetic Yeast Prions

Hsp40/JDP Requirements for the Propagation of Synthetic Yeast Prions

GmDNJ1, a type‐I heat shock protein 40 (HSP40), is responsible for both Growth and heat tolerance in soybean

The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis

Contact Info

Product

Resources

About