The expansion and diversity of the<i>CYP75</i>gene family in Vitaceae

Xiao, Yang; Wen, Jun; Meng, Ran; Meng, Ying; Zhou, Qiang; Nie, Ze‐Long

doi:10.7717/peerj.12174

Cited by 9 publications

(6 citation statements)

References 62 publications

(102 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phylogenetic analysis of existing sequences of CYP75 s revealed that CYP75A was derived from CYP75B prior to the divergence of angiosperms and gymnosperms ( Seitz et al., 2006 ). It is demonstrated that the CYP75B subfamily has perhaps a more superior evolutionary position in the CYP75 gene family, performing not only in its own function, but sometimes work as compensation for the CYP75A subfamily ( Xiao et al., 2021 ). In this study, all orchid CYP75 s were found to be located on the endoplasmic reticulum, as previous research has shown that CYP450 is a membrane protein primarily found on the ER membrane in eukaryotes ( Brignac-Huber et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-based identification of the CYP75 gene family in Orchidaceae and its expression patterns in Cymbidium goeringii

Li,

Zhao,

Zhang

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

With a great diversity of species, Orchidaceae stands out as an essential component of plant biodiversity, making it a primary resource for studying angiosperms evolution and genomics. This study focuses on 13 published orchid genomes to identify and analyze the CYP75 gene family belonging to the cytochrome P450 superfamily, which is closely related to flavonoid biosynthetic enzymes and pigment regulation. We found 72 CYP75s in the 13 orchid genomes and further classified them into two classes: CYP75A and CYP75B subfamily, the former synthesizes blue anthocyanins, while the latter is involved in the production of red anthocyanins. Furthermore, the amount of CYP75Bs (53/72) greatly exceeds the amount of CYP75As (19/72) in orchids. Our findings suggest that CYP75B genes have a more important evolutionary role, as red plants are more common in nature than blue plants. We also discovered unique conserved motifs in each subfamily that serve as specific recognition features (motif 19 belong to CYP75A; motif 17 belong to CYP75B). Two diverse-colored varieties of C. goeringii were selected for qRT-PCR experiments. The expression of CgCYP75B1 was significantly higher in the purple-red variant compared to the yellow-green variant, while CgCYP75A1 showed no significant difference. Based on transcriptomic expression analysis, CYP75Bs are more highly expressed than CYP75As in floral organs, especially in colorful petals and lips. These results provide valuable information for future studies on CYP75s in orchids and other angiosperms.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-based identification of the CYP75 gene family in Orchidaceae and its expression patterns in Cymbidium goeringii

Li,

Zhao,

Zhang

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Based on in the Swiss-Prot database, their functional domain was confirmed in silico as being compatible with the P450 superfamily. The CYP75A ( F3'5'H ) and CYP75B ( F3'H ) subfamilies can catalyze the conversion of apigenin to luteolin (KEGG: map0094) and perform hydroxylation of the B ring of flavonoids, important for the biosynthesis of blue and red anthocyanins (Xiao et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant

et al. 2022

View full text Add to dashboard Cite

Erythrina velutina is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism in plants inhabiting the harsh conditions of the Caatinga, the present study focused on this important class of bioactive phenolics. Leaves and seeds of plants growing in their natural habitat had their metabolic and proteomic profiles analyzed and integrated with transcriptome data. As a result, 96 metabolites (including 43 flavonoids) were annotated. Transcripts of the flavonoid pathway totaled 27, of which EvCHI, EvCHR, EvCHS, EvCYP75A and EvCYP75B1 were identified as putative main targets for modulating the accumulation of these metabolites. The highest correspondence of mRNA vs. protein was observed in the differentially expressed transcripts. In addition, 394 candidate transcripts encoding for transcription factors distributed among the bHLH, ERF, and MYB families were annotated. Based on interaction network analyses, several putative genes of the flavonoid pathway and transcription factors were related, particularly TFs of the MYB family. Expression patterns of transcripts involved in flavonoid biosynthesis and those involved in responses to biotic and abiotic stresses were discussed in detail. Overall, these findings provide a base for the understanding of molecular and metabolic responses in this medicinally important species. Moreover, the identification of key regulatory targets for future studies aiming at bioactive metabolite production will be facilitated.

show abstract

“…where T、P are the temperature and pressure of the gas-liquid interface grid cell,  is a dimensionless coefficient related to the diameter [32][33][34]. Based on the classical Lee model, a gas-liquid phase change mass transfer model that considers bubble parameter factors can be derived.…”

Section: The Basic Physical Modelmentioning

confidence: 99%

Research on convective cooling and thermal protection characteristics of integrated dual-sided deflector system

Zhang,

Jiang,

Deng

2024

Preprint

View full text Add to dashboard Cite

Based on the goals of "high reliability, high frequency, rapid launch, and low cost" for space launch site, and drawing inspiration from the design principles of SpaceX's Starship launch test facility, an integrated dual-sided deflector system for convective cooling and thermal protection is presented. The interaction process between the gas jet and liquid water jet and its effect on the flow field environment are thoroughly studied using numerical calculation methods. Furthermore, Regarding the phase-change heat transfer issue in a compressible gas-liquid two-phase flow and the varying distribution of different bubble shapes and sizes at the gas-liquid interface, a modified Lee model is derived. The rationality and accuracy of the phase-change mass-transfer coefficient values can be improved by adjusting the equivalent diameter and shape factor of the bubbles. The research results demonstrate that compared to the classical Lee model, the modified Lee model can achieve a higher numerical accuracy in predicting the heat and mass transfer processes in gas-liquid two-phase flows. Through comparative analysis with traditional dual-sided deflector and conventional cooling system, the integrated dual-sided deflector system for convective cooling and thermal protection exhibits significant performance advantages in gas flow regulation, flow field environment improvement and erosion protection in the near-ground region of the launch site. Particularly, regarding the thermal environment of the dual-sided deflector, it not only achieves effective flow deflection, but also mitigates the degree of erosion caused by the gas jet on the deflector. This conclusion can provide theoretical references for the thermal protection design of commercial launch vehicle system at space launch site.

show abstract

The expansion and diversity of theCYP75gene family in Vitaceae

Cited by 9 publications

References 62 publications

Genome-based identification of the CYP75 gene family in Orchidaceae and its expression patterns in Cymbidium goeringii

Genome-based identification of the CYP75 gene family in Orchidaceae and its expression patterns in Cymbidium goeringii

Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina Willd., a Brazilian semiarid native woody plant

Research on convective cooling and thermal protection characteristics of integrated dual-sided deflector system

Contact Info

Product

Resources

About