The experimental use of cortisone in peripheral nerve repair with plasma clot as a suture

Experimental Study of Healing of Trans-Sected Peripheral NervesSummary. The behavior of the connective tissue during the healing of transsected nerves was studied. Experiments were carried out with rabbits. The sciatic nerve was trans-seeted and repaired using different techniques. Postoperatively the animals were all treated in exactly the same way. The clinical course was registrated carefully, with special attention to the return of the motor function and the occurence and extension of decubital ulcers. Finally the animals were sacrified, and the site of nerve repair was excised and histologically examined using H~matoxilin, Eosin. Van Gieson, Kresylviolett, Kliiver-Barriera, Sudan-Schwarz B, Bodian staining.Two different techniques were compared by operating on the two sciatique nerves of the same animal. Individual factors could be excluded as the result of each two operations was compared in the same individual.A. In 32 rabbits, 64 sciatic nerves were repaired by epineural end-to-end suture (Mersilen 6 • 0) in order to study the timing of the healing process.

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Experimental study of healing of trans-sected peripheral nerves

Millesi

1972

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The effect of Mannose-6-Phosphate on recovery after sciatic nerve repair

et al. 2011

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We have determined the effect of applying Mannose-6-Phosphate (M6P), a scar reducing agent, to a site of sciatic nerve repair. In anaesthetised C57-Black-6 mice, the left sciatic nerve was sectioned and repaired using 4 epineurial sutures. Either 100 μl of 600 mM Mannose-6-Phosphate (29 animals), or 100 μl of phosphate buffered saline as a placebo control (29 animals), was injected into and around the nerve repair site. A further group acted as sham-operated controls. After 6 or 12 weeks of recovery the extent of regeneration was assessed electrophysiologically and the percentage area of collagen staining at the repair site was analysed using picrosirius red and image analysis. Gait analysis was undertaken pre-operatively and at 1, 3, 6, 9 and 12 weeks postoperatively, to assess functional recovery. At 6 weeks the compound action potentials recorded from the regenerated nerves in the M6P group were significantly larger than in the placebo controls (P=0.015), and the conduction velocities were significantly faster (P=0.005), but there were no significant differences between these groups at 12 weeks. Gait analysis suggested better early functional recovery in the M6P group. In both repair groups there was a significant reduction in collagen staining between 6 and 12 weeks, suggestive of scar remodelling. We conclude that the normal scar remodelling process aids long term recovery in repaired nerves. Administration of 600 mM M6P to the nerve repair site enhances nerve regeneration and functional recovery in the early stages, and may lead to improved outcomes.

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The experimental use of cortisone in peripheral nerve repair with plasma clot as a suture

Cited by 2 publications

References 8 publications

Experimental study of healing of trans-sected peripheral nerves

Experimental study of healing of trans-sected peripheral nerves

The effect of Mannose-6-Phosphate on recovery after sciatic nerve repair

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