The exploration of droplet digital branched rolling circle amplification based ultrasensitive biosensor for gastric cancer cell-derived extracellular vesicles detection

Huang, Rongrong; Di, Kaili; Adeel, K.; Fan, Boyue; Gu, Xinrui; Xu, Hao; Shen, Hanfei; He, Nongyue; Li, Z.

doi:10.1016/j.mtadv.2022.100296

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…The single molecular amplification of DNA by droplet microfluidic hyper branched RCA has been developed. Nowadays, droplet digital RCA systems have been designed for the sensitive and rapid detection of cancerous cell-derived extracellular vesicles [ 49 ]. An electrochemical aptasensor (ultrasensitive) was developed for multiple exosome biomarker detection in breast cancer, which was based on dual RCA [ 50 ].…”

Section: Microfluidic Technology and Poctsmentioning

confidence: 99%

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

et al. 2023

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Conventional diagnostic techniques are based on the utilization of analyte sampling, sensing and signaling on separate platforms for detection purposes, which must be integrated to a single step procedure in point of care (POC) testing devices. Due to the expeditious nature of microfluidic platforms, the trend has been shifted toward the implementation of these systems for the detection of analytes in biochemical, clinical and food technology. Microfluidic systems molded with substances such as polymers or glass offer the specific and sensitive detection of infectious and noninfectious diseases by providing innumerable benefits, including less cost, good biological affinity, strong capillary action and simple process of fabrication. In the case of nanosensors for nucleic acid detection, some challenges need to be addressed, such as cellular lysis, isolation and amplification of nucleic acid before its detection. To avoid the utilization of laborious steps for executing these processes, advances have been deployed in this perspective for on-chip sample preparation, amplification and detection by the introduction of an emerging field of modular microfluidics that has multiple advantages over integrated microfluidics. This review emphasizes the significance of microfluidic technology for the nucleic acid detection of infectious and non-infectious diseases. The implementation of isothermal amplification in conjunction with the lateral flow assay greatly increases the binding efficiency of nanoparticles and biomolecules and improves the limit of detection and sensitivity. Most importantly, the deployment of paper-based material made of cellulose reduces the overall cost. Microfluidic technology in nucleic acid testing has been discussed by explicating its applications in different fields. Next-generation diagnostic methods can be improved by using CRISPR/Cas technology in microfluidic systems. This review concludes with the comparison and future prospects of various microfluidic systems, detection methods and plasma separation techniques used in microfluidic devices.

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Section: Microfluidic Technology and Poctsmentioning

confidence: 99%

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

et al. 2023

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“…Assisted by digital microuidics, this sensing method could perform ultrasensitive quantication of cancer EVs and improve the LOD down to 12 particle per mL. 51 The combination of aptamers and nanoparticles has been proven to be an effective approach for signal amplication. Nanoparticles can offer a large surface area for the conjugation of multiple copies of the targeting moiety onto one carrier, which simultaneously enhances the specic targeting capability and signal strength.…”

Section: Fluorescence Aptasensorsmentioning

confidence: 99%

“…Assisted by digital microfluidics, this sensing method could perform ultrasensitive quantification of cancer EVs and improve the LOD down to 12 particle per mL. 51…”

Section: Aptamer-based Diagnosismentioning

confidence: 99%

Aptamer-based technology for gastric cancer theranostics

Cui

Song

2023

Anal. Methods

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“…Nowadays, droplet digital RCA systems have been developed for sensitive and rapid detection of cancerous cells derived extracellular vesicles. [33] An electrochemical aptasensor (ultrasensitive) was developed for multiple exosome biomarker detection in breast cancer which was based on dual rolling circle amplification. [34]…”

Section: Rolling Circle Amplification (Rca)mentioning

confidence: 99%

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

Mumtaz¹,

Rashid²,

Ali³

et al. 2023

Preprint

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Conventional diagnostic techniques are based on utilization of analyte sampling, sensing and signalling on separate platforms for detection purposes, that must be evaded and integrated to a single step procedure in point of care (POC) testing devices. Therefore, the trend has been shifted towards utilization of microfluidic platforms for detection of analytes in biochemical, clinical and food technology due to its being expeditious. Microfluidic systems moulded with polymer substances or glass offer specific and sensitive detection of infectious and non-infectious diseases by providing innumerable benefits including less cost, good biological affinity, strong capillary action and simple process of fabrication. In the case of nucleic acid-based nanosensors, there are some challenges that need to be addressed such as cellular lysis, isolation and amplification of nucleic acid required to be accomplished prior to its detection. To avoid utilization of laborious steps for executing these steps, advances have been deployed in this perspective for on-chip sample preparation, amplification and detection which not only improves sensitivity and selectivity but also saves time and resources. This review emphasizes the significance of microfluidic technology for nucleic acid detection of infectious and non-infectious diseases. The implementation of isothermal amplification in concomitance with lateral flow assay greatly increases the binding efficiency of nanoparticles and biomolecules, improves limit of detection and sensitivity. Most importantly deployment of paper-based material made of cellulose reduces the overall cost. Microfluidic technology in nucleic acid testing has been discussed by explicating its applications in different fields. This review concludes with the prospects and proposes future directions in microfluidic based methods in disease diagnosis.

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The exploration of droplet digital branched rolling circle amplification based ultrasensitive biosensor for gastric cancer cell-derived extracellular vesicles detection

Cited by 6 publications

References 30 publications

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

Aptamer-based technology for gastric cancer theranostics

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

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