The expression characteristics of methyl jasmonate biosynthesis–related genes in Cymbidium faberi and influence of heterologous expression of CfJMT in Petunia hybrida

Tian, Chunling; Liu, Song; Jiang, Li; Tian, Silin; Wang, Guangdong

doi:10.1016/j.plaphy.2020.03.051

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…The error bars indicate three RT-qPCR biological replicates. The values were standardized by the C. faberi reference gene GAPDH (GenBank Accession: JX560732; Tian et al, 2020 ). The expression values of CfTPS genes are listed in Supplementary Table S11 , and the RT-qPCR primers of CfTPS are listed in Supplementary Table S12 .…”

Section: Resultsmentioning

confidence: 99%

“…16 PerfectStart™ Green qPCR SuperMix (TransGen Biotech, Beijing, China) was used for RT-qPCR analysis. The C. faberi reference gene GAPDH (GenBank Accession: JX560732; Tian et al, 2020 ) was used as the internal control and quantified by the 2 -△△CT method ( Livak and Schmittgen, 2001 ). There were three biological replicates in the RT-qPCR analysis.…”

Section: Methodsmentioning

confidence: 99%

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Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

et al. 2021

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Terpene synthases (TPSs) are essential for forming terpenes, which play numerous functional roles in attracting pollinators, defending plants, and moderating the interaction between plants. TPSs have been reported in some orchids, but genome-wide identification of terpenes in Cymbidium faberi is still lacking. In this study, 32 putative TPS genes were classified in C. faberi and divided into three subfamilies (TPS-a, TPS-b, and TPS-e/f). Motif and gene structure analysis revealed that most CfTPS genes had the conserved aspartate-rich DDxxD motif. TPS genes in the TPS-a and TPS-b subfamilies had variations in the RRX8W motif. Most cis-elements of CfTPS genes were found in the phytohormone responsiveness category, and MYC contained most of the numbers associated with MeJA responsiveness. The Ka/Ks ratios of 12/13 CfTPS gene pairs were less than one, indicated that most CfTPS genes have undergone negative selection. The tissue-specific expression patterns showed that 28 genes were expressed in at least one tissue in C. faberi, and TPS genes were most highly expressed in flowers, followed by leaves and pseudobulbs. In addition, four CfTPS genes were selected for the real-time reverse transcription quantitative PCR (RT-qPCR) experiment. The results revealed that CfTPS12, CfTPS18, CfTPS23, and CfTPS28 were mainly expressed in the full flowering stage. CfTPS18 could convert GPP to β-myrcene, geraniol, and α-pinene in vitro. These findings of CfTPS genes of C. faberi may provide valuable information for further studies on TPSs in orchids.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

et al. 2021

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“…JMT represents the key enzyme for MeJA biosynthesis, which catalyzes the production of MeJA from jasmonic acid [ 16 , 17 ]. To date, only two JMTs that contribute to floral MeJA production have been characterized [ 11 , 14 ]. In C. ensifolium , the CeJMT gene displayed floral developmental and diurnal rhythmic expression, and its recombinant protein could catalyze jasmonic acid to form MeJA [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…MeJA is a common component of floral volatiles and is initially identified from the flowers of Jasminum grandiflorum [ 9 ]. It often emerges in the floral scent profiles of ornamental plants, such as Cymbidium ensifolium [ 10 ], C. faberi [ 11 ], Paeonia ostia [ 12 ], and Hedychium coronarium [ 13 ]. In C. ensifolium , MeJA accounts for the largest proportion of floral volatiles, and its emission is perianth-specific and floral development-regulated [ 10 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…The JMTs responsible for MeJA biosynthesis have been characterized in several plants [ 18 , 19 , 20 , 21 ]. However, the JMTs that contribute to MeJA production in flowers were only characterized in C. ensifolium and C. faberi [ 11 , 14 ]. Functional characterization in vitro showed that CeJMT can synthesize MeJA from jasmonic acid and S-adenosyl-L-methionine (SAM) substrates.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Jasmonic Acid Methyltransferase Involved in the Formation of Floral Methyl Jasmonate in Hedychium coronarium

Yue,

Zhang,

Wang

et al. 2023

Plants

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Hedychium coronarium is a popular ornamental flower in tropical and subtropical areas due to its elegant appearance and inviting fragrance. Methyl jasmonate (MeJA) is one of the volatile compounds in the blooming flowers of H. coronarium. However, the molecular mechanism underlying floral MeJA formation is still unclear in H. coronarium. In this study, a total of 12 SABATH family genes were identified in the genome of H. coronarium, and their encoded proteins range from 366 to 387 amino acids. Phylogenetic analysis revealed seven clades in the SABATH family and a JMT ortholog clade, including two HcSABATH members. Combined with expression profiling of HcSABATH members, HcJMT1 was identified as the top candidate gene for floral MeJA biosynthesis. In vitro enzyme assays showed that HcJMT1 can catalyze the production of MeJA from jasmonic acid. Gene expression analysis indicated that HcJMT1 exhibited the highest expression in the labella and lateral petals, the major sites of MeJA emission. During flower development, the two MeJA isomers, major isomers in the products of the HcJMT1 protein, were released after anthesis, in which stage HcJMT1 displayed high expression. Our results indicated that HcJMT1 is involved in the formation of floral MeJA in H. coronarium.

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Transcriptomic and metabolomic perspectives for the growth of alfalfa (Medicago sativa L.) seedlings with the effect of vanadium exposure

Gan

Zhang

et al. 2023

Chemosphere

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The expression characteristics of methyl jasmonate biosynthesis–related genes in Cymbidium faberi and influence of heterologous expression of CfJMT in Petunia hybrida

Cited by 6 publications

References 42 publications

Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

Identification and Characterization of Jasmonic Acid Methyltransferase Involved in the Formation of Floral Methyl Jasmonate in Hedychium coronarium

Transcriptomic and metabolomic perspectives for the growth of alfalfa (Medicago sativa L.) seedlings with the effect of vanadium exposure

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