The expression, localization, and effect of a human interferon in plants

Zoeten, G. A. de; Penswick, John Robert; Horisberger, Michel A.; Ahl, Patricia; Schultze, Michael; Höhn, Thomas

doi:10.1016/0042-6822(89)90123-2

Cited by 121 publications

(55 citation statements)

References 18 publications

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“…Among the notable achievements were the ¢rst transfection of plant protoplasts with infectious RNA transcripts of brome mosaic virus (Ahlquist et al 1984), the synthesis of recombinant marker proteins in cells and inoculated leaves (Brisson et al 1984;French et al 1986;Takamatsu et al 1987;Dawson et al 1988) and the production of measurable quantities of a cytokine with biological activity (de Zoeten et al 1989). However, the use of recombinant plant viruses as research and production tools for plant biotechnology was not ¢rmly established until tobamovirus vectors were used in a commercial context to transfect whole plants and the resulting products puri¢ed on a pilot scale and analysed for their composition and speci¢c activity.…”

Section: Introductionmentioning

confidence: 99%

Tobacco mosaic virus and the virescence of biotechnology

Turpen¹

1999

Phil. Trans. R. Soc. Lond. B

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There is a growing realization that a modern combination of molecular biology and agriculture will provide a photosynthetic basis for the biosynthesis of an increasing variety of complex and valuable molecules. This`greening' of biotechnology may impact on the global environment in many bene¢cial ways, but will perhaps have its most signi¢cant impact on human health. In the past decade, the capacity to use plants as an expanded source of therapeutics has grown through the accelerated development of e¡ective viral transfection vectors for gene transfer to cultivated crops. Recombinant vectors based on tobacco mosaic virus (TMV) and other members of the Tobamovirus genus are now used to transfect commercially meaningful quantities of plant biomass cultivated in enclosed greenhouses and multiacre ¢elds. Viral RNA promoters are e¡ectively manipulated for the synthesis of recombinant messenger RNAs in whole plants. Chimeric plant virus and virus-like particles are designed for peptide production and display from recombinant structural protein^gene fusions. Gene functions are assessed and modi¢ed by either virus-mediated expression or cytosolic inhibition of expression at the RNA level. Recombinant virus populations, propagated by inoculating plants with infectious RNA transcripts or recombinant virions, have proved to be genetically stable over product-manufacturing cycles. Large volumes of highly puri¢ed protein products isolated from transfected foliage conform reproducibly to the speci¢cations required for well-characterized biologics. In some cases, they exceed the speci¢c activities of molecules puri¢ed from alternative recombinant and native sources. The resulting products are then formulated according to the developing national regulatory guidelines appropriate for agriculture-based manufacturing. Each of these innovations was ¢rst realized by researchers using clones of tobamovirus genes and recombinant genomes. This progress is founded on the heritage of a century of fundamental TMV research.

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Section: Introductionmentioning

confidence: 99%

Tobacco mosaic virus and the virescence of biotechnology

Turpen¹

1999

Phil. Trans. R. Soc. Lond. B

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“…planta-pharma, plant bioreactors, plant biofactory, 그리고 pharmaceutical gardening 등 많은 용어들이 비슷한 의미로 혼용하여 사용하고 있다 (Basaran and Rodriguez-Cerezo 2008 (Mason et al 1998;Kang et al 2004), 유전자의 안정성에 영향을 미 치는 요소 제거 (De Jaeger et al 2002;Ibrahim et al 2001;Outchkourov et al 2003) (Barta et al 1986;De Zoeten et al 1989;Sijmons et al 1990), 1989년에는 담배의 잎에서 항체를 발현을 시킴으로서 식물에서 복잡 한 형태의 치료용 동물단백질을 생산할 수 있다는 가능성 이 제시되었다 (Hiatt et al 1989 (Poirier et al 1992).…”

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Current status in molecular farming

Kim¹,

Yang

2010

Journal of Plant Biotechnology

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Molecular farming is production of pharmaceutically and industrially important proteins in plants. Plants and plant cell culture systems have been used as bio-factory to produce recombinant proteins such as monoclonal antibodies, enzymes, vaccines, hormones, interleukins, commercial enzymes and etc. The terms molecular farming, biofarming, molecular pharming, phytomanufacturing, recombinant or plant-made industrials, planta-pharma, plant bioreactors, plant biofactory, and pharmaceutical gardening are used interchangeably. Molecular farming can provide safe and inexpensive pharmaceutical proteins as well as commercial ones. In spite of several advantages of molecular farming such as safety and inexpensive cost, there are also a couple of drawbacks in the existing technology. One of them is low expression level of target gene in plants, which has been improved by optimizing gene-based codon usage, screening of strong promoters, expression of transcription factors, subcellular targeting of target proteins, chloroplast transformation, and transient expression using viral expression system (magnifection). Some plant-based commercial proteins have already been in markets and more than twenty plant-based pharmaceuticals have been in clinical trials, from that we can expect that several plant-based pharmaceutical proteins will be seen in the markets in the near future.

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“…While these early vectors were useful in plant cell systems to produce recombinant protein products with potential market value [16], these early systems could not support large scale manufacturing nor did they exploit the advantages of agriculture to provide cost-effective products. This review will provide an overview of plant virus-based expression vectors, and provide select examples how virus expression systems have evolved to offer valuable tools for the production of medically important products [17] and support the study of plant structure and metabolic function (reviewed in [18]) in dicot-and monocotyledonous plants.…”

Section: Introductionmentioning

confidence: 99%