The expression of CD11/CD18 molecules on rabbit leucocytes: Identification of monoclonal antibodies to CD18 and their effect on cellular adhesion processes

Galea‐Lauri, Joanna; Blackford, J; Wilkinson, J M

doi:10.1016/0161-5890(93)90027-9

Cited by 19 publications

(18 citation statements)

References 30 publications

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“…After adhering, the myeloma cells could be fixed to plates with methanol and stained with amido black. Methanol fixation and amido black staining of neutrophils did not work in the present study and the best fixation and staining method for neutrophils was 0·5% glutaraldehyde and 1% crystal violet, respectively, similar to that reported by Galea-Lauri et al (1993). St John et al (1994 demonstrated nonstimulated adhesion of myeloma cell lines to fibronectin, laminin, and bovine GN; however, they obtained less than 5% cell adhesion to BSA.…”

Section: Discussionsupporting

confidence: 64%

“…However, because catfish neutrophils do not constitutively adhere to BSA, LPS, serum, or P-GN in large numbers there appears to be a causal relationship between neutrophil stimulation and adhesion. These results are not meant to imply that there are specific receptors for BSA on catfish neutrophils, however, there may be a GN and LPS binding receptor on catfish neutrophils similar to the receptors found on mammalian macrophages and neutrophils Galea-Lauri et al, 1993;Detmers et al, 1994). The adhesion of rabbit neutrophils to gelatin-coated wells has been reported to be CD18-dependent (Nourshargh et al, 1989), whether this is the case in channel catfish remains to be proven.…”

Section: Discussionmentioning

confidence: 86%

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Increased adhesion of channel catfish,Ictalurus punctatusRafinesque, neutrophils to substrates following stimulation with phorbol ester

Ainsworth

Qian

Boyd

et al. 1996

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 86%

Increased adhesion of channel catfish,Ictalurus punctatusRafinesque, neutrophils to substrates following stimulation with phorbol ester

Ainsworth

Qian

Boyd

et al. 1996

Fish & Shellfish Immunology

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“…We have previously compared the N-terminal sequence data for rabbit and human CDllb [40] and this is shown in Fig. 4, together with that for human CDlla.…”

Section: F N L E T E N T M T F (S) X N E X X (F) X (0mentioning

confidence: 97%

A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM‐1 is a ligand for CD11c/CD18

Blackford

Reid

Pappin³

et al. 1996

Eur J Immunol

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The rabbit CD11c molecule has been characterized by use of new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes in peripheral blood. No expression on neutrophils could be demonstrated. Incubation of the newly derived CD8+ T cell line, BJ/873, with mAb 3/22 causes homotypic aggregation, which has been shown to be cell surface event that is not dependent on intracellular signaling or on receptor cross-linking. Inhibition studies show that the ligands responsible for this aggregation are CD11c/CD18 and ICAM-1, both of which are expressed on BJ/873. One other rabbit T cell line, K34, that also expresses p150,95 and ICAM-1, shows a similar aggregation response when stimulated with 3/22. Cell lines that express p150,95 but not ICAM-1 do not aggregate. These observations suggest that ICAM-1 is a ligand for activated p150,95.

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“…Vessel segments were briefly equilibrated in 30% sucrose in PBS at 4°C, embedded in optimal cutting temperature compound (Miles Scientific), frozen in liquid nitrogen, and sectioned (6 m) onto Immunohistochemistry to identify CD18 ϩ leukocytes, T lymphocytes, or macrophages was performed using primary antibodies directed against rabbit CD18 (Serotec), 19 rabbit CD43 (T11/135, Serotec), 7 or rabbit RAM 11 (Dako), 19 respectively. VCAM-1 and ICAM-1 were identified using monoclonal antibodies raised against rabbit VCAM-1 and ICAM-1 20 (a generous gift of Dr M. Cybulsky, University of Toronto).…”

Section: Immunohistochemistry and Image Analysismentioning

confidence: 99%

Acute Host-Mediated Endothelial Injury After Adenoviral Gene Transfer in Normal Rabbit Arteries

Channon

Qian

Youngblood

et al. 1998

Circulation Research

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Abstract-Acute injury after adenoviral vascular gene transfer remains incompletely characterized. Here, we describe the early response (Յdays) in 52 New Zealand White rabbits undergoing gene transfer (␤-galactosidase or empty vector) or sham procedures to both carotid arteries. After gene transfer, arteries were either left in vivo for 1 hour to 3 days (in vivo arteries) or were excised immediately after gene transfer and cultured (ex vivo arteries). Within 1 hour, in vivo arteries receiving infectious titers of Ն4ϫ10 9 plaque-forming units (pfu)/mL showed endothelial activation, with an acute inflammatory infiltrate developing by 6 hours. Ex vivo arteries showed endothelial activation but no inflammatory infiltrate. There were also significant differences in transgene expression between in vivo and ex vivo arteries. Ex vivo arteries showed titer-dependent increases in ␤-galactosidase expression through 2ϫ10 10 pfu/mL, whereas in in vivo arteries, titers above 4ϫ10 9 pfu/mL merely increased acute inflammatory response, without increasing transgene expression. In vivo arteries showed significant time-and titer-dependent impairment in endothelium-dependent relaxation, with no effect on contraction or nitroprusside-induced relaxation. Interestingly, however, if rabbits were made neutropenic with vinblastine, their arteries maintained full endothelium-dependent relaxation, even after very high titer vascular infection (up to 1ϫ10 11 pfu/mL). These findings show that recombinant adenovirus triggers an early inflammatory response, and it is the inflammatory response that in turn causes functional endothelial injury. This occurs at much lower titers than previously appreciated (though the precise threshold will undoubtedly vary between laboratories). However, titers below the inflammatory threshold produce excellent transgene expression without inflammation or vascular injury. (Circ Res. 1998;82:1253-1262.)

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The expression of CD11/CD18 molecules on rabbit leucocytes: Identification of monoclonal antibodies to CD18 and their effect on cellular adhesion processes

Cited by 19 publications

References 30 publications

Increased adhesion of channel catfish,Ictalurus punctatusRafinesque, neutrophils to substrates following stimulation with phorbol ester

Increased adhesion of channel catfish,Ictalurus punctatusRafinesque, neutrophils to substrates following stimulation with phorbol ester

A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM‐1 is a ligand for CD11c/CD18

Acute Host-Mediated Endothelial Injury After Adenoviral Gene Transfer in Normal Rabbit Arteries

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