The Expression of Circulating miR-497 and Metadherin in Hepatocellular Carcinoma: Relation to the Tumor Characteristics and Patients’ Survival

Abstract

Objectives: This study aimed to evaluate the prognostic significance and relationship of miR-497 and metadherin to hepatocellular carcinoma (HCC) tumor characteristics and patients’ survival. Methods: This study enrolled 120 (60 HCC patients and 60 healthy) subjects. Serum miR-497 and metadherin mRNA relative expression were analyzed by real-time quantitative reverse transcription polymerase chain reaction. The overall survival (OS) of HCC patients was assessed using the Kaplan–Meier curve and log-rank test. Results: Serum miR-497 showed statistically significant downregulation in HCC patients compared to controls (p < 0.001). Serum metadherin mRNA relative expression was significantly upregulated in HCC patients compared to controls (p < 0.001). Both serum miR-497 and metadherin mRNA expression were significantly associated with the number of tumor foci (p = 0.028 and 0.001, respectively), tumor size (p = 0.022 and <0.001, respectively), nodal metastasis (p = 0.003 and 0.003, respectively), distant metastasis (p = 0.003 and 0.003, respectively), vascular invasion (p = 0.040 and <0.001, respectively), and BCLC staging (p = 0.043 and 0.004, respectively). The overall survival was lower in patients with low miR-497 expression (p = 0.046) and in patients with high metadherin expression (p < 0.001). Conclusion: The expression levels of miR-497 showed downregulation in HCC patients, but metadherin expression showed upregulation. Both markers were inversely related and closely correlated with tumor characteristics and patients’ survival.

A Meta-Analysis of MiRNA-497 and Prognosis of Hepatocellular Carcinoma

“…After removing 31 duplicate articles, the remaining 128 articles were screened according to inclusion and exclusion criteria, and 21 full-text articles were further reviewed. Finally, 6 studies with NOS scores greater than 6 were included after detailed screening [13,[17][18][19][20][21]. HR and 95% CI of two studies were extracted from the survival curve according to the method provided by Jayne F.T et al Te six included studies were all published between 2016 and 2021, including 457 patients.…”

“…Tus, the role of miR-497 in the prognosis of patients with HCC has attracted much attention. Some studies have shown that the low expression level of miR-497 is signifcantly correlated with the poor prognosis of HCC [13,[17][18][19][20][21]. However, these studies have their limitations, such as small sample sizes or controversial results.…”

Roles and Molecular Mechanisms of Biomarkers in Hepatocellular Carcinoma with Microvascular Invasion: A Review

“…Later studies showed that MTDH was abnormally expressed in various cancers, including esophageal, breast, gastric, liver cancer, osteosarcoma, and others. 84 – 89 In HCC cells, MTDH expression was significantly higher than that in adjacent nontumor tissues. Pathologically, MTDH level was positively correlated with pathologic satellites, advanced TNM stages and MVI ( p <0.001).…”