The Expression of Cytokine Profiles and Related Receptors in Idiopathic Inflammatory Myopathies

Zhou, Junyu; Zhao, Lijuan; Ye, Xiao; Xie, Shasha; Long, Ying; Yu, Wei; Meng, Qiming; Li, Xiaojing; Luo, Hui; Zhu, Honglin

doi:10.3389/fphar.2022.852055

Cited by 9 publications

(9 citation statements)

References 45 publications

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“…This finding is consistent with the positive correlation between serum CK levels and ASMI scores in the present study (Figure 1b) as well as the results from our logistic regression analysis, which identified CK levels < 120 U/L as a risk factor for sarcopenia. Furthermore, CK levels were positively associated with serum levels of IL-7 and CCL3 (Figure 1c,d), which have been previously implicated in the regulation of human skeletal muscle stem cells and muscle tissue regeneration [39,40]. Thus, increased skeletal muscle mass, activity, and subsequent microscopic injuries may lead to elevated serum levels of CK.…”

Section: Discussionmentioning

confidence: 69%

Associations of Serum CXCL12α and CK Levels with Skeletal Muscle Mass in Older Adults

Chen,

Laurentius,

Fait

et al. 2023

JCM

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Sarcopenia, a condition characterized by gradual loss of skeletal muscle mass and function, is a complex diagnosis; the decisive criterion in this diagnosis is the measurement of appendicular skeletal muscle index (ASMI). To identify potential serum markers predictive of sarcopenia in older adults, we evaluated correlations between ASMI, clinical data, and 34 serum inflammation markers in 80 older adults. Pearson’s correlation analyses confirmed that ASMI was positively correlated with nutritional status (p = 0.001) and serum creatine kinase (CK) (p = 0.019) but negatively correlated with serum CXCL12α (p = 0.023), a chemoattractant for muscle stem cells. In the case group, ASMI was negatively correlated with serum interleukin (IL)-7 (p = 0.024), a myokine expressed and secreted from skeletal muscle cells in vitro. Multivariate binary logistic regression analyses identified four risk factors for sarcopenia in our study: advanced age (p = 0.012), malnutrition (p = 0.038), low serum CK levels (p = 0.044), and high serum CXCL12α levels (p = 0.029). Low CK and high CXCL12α levels serve as combinatorial serum markers of sarcopenia in older adults. The linear correlation between ASMI and CXCL12α levels may facilitate the development of new regression models for future studies on sarcopenia.

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Section: Discussionmentioning

confidence: 69%

Associations of Serum CXCL12α and CK Levels with Skeletal Muscle Mass in Older Adults

Chen,

Laurentius,

Fait

et al. 2023

JCM

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“…Serum IL-1ra was significantly elevated in the DM-active subset than in the DM-stable subset ( 48 ). Zhou et al ( 14 ) also reported higher serum levels of IL-1ra in anti-MDA5 DM when compared with ASS. It was proposed that elevated serum IL-1ra in anti-NXP2 DM might switch from a controlled and protective immune role to one in which this protein promotes inflammatory tissue damage in severe cases ( 49 ).…”

Section: Discussionmentioning

confidence: 92%

“…Although sarcoplasmic MxA expression has a high sensitivity for the diagnosis of DM (71%−77%) ( 5 , 13 ), muscle biopsy is not yet fully available. Despite the increasing number of studies on serum levels of cytokines/chemokines in patients with DM and ASS ( 14 – 16 ), there is still no consensus on the differences in these mediators between these two diseases. The identification of biomarkers could lead to a better understanding of the pathogenesis of diseases and discrimination between DM and ASS.…”

Section: Introductionmentioning

confidence: 99%

Comparison of cytokine/chemokine profiles between dermatomyositis and anti-synthetase syndrome

et al. 2022

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ObjectivesDermatomyositis (DM) and anti-synthetase syndrome (ASS) are autoimmune diseases with multisystem involvement. Despite sharing some clinical and myopathological features, these are two diseases with different pathogeneses and prognoses. We aimed to clarify and compare cytokine/chemokine profiles in both disorders, which may help in the differential diagnosis.Materials and methodsWe collected clinical data and serum samples of consecutive patients with DM and ASS. Quantibody® Human Inflammation Array 3 for cytokines/chemokines was performed in the serum of all participants. Receiver operating characteristic analysis with the area under the curve and Youden's index were performed.ResultsEight newly diagnosed and treatment-naïve patients with DM, nine newly diagnosed and treatment-naïve patients with ASS, and 14 healthy controls were enrolled. Serum C-C motif chemokine ligand (CCL) 2, CCL4, C-X-C motif chemokine ligand (CXCL) 13, and tumor necrosis factor receptor 2 (TNFR2) were increased in patients with both DM and ASS. Serum interleukin (IL)-1 receptor type 1 (IL-1ra), IL-1b, CCL1, CXCL11, and CCL3 were modulated in patients with DM only, and IL-8, CXCL9, and tissue inhibitors of metalloproteinases-1 (TIMP-1) in patients with ASS only. Serum CCL2, CXCL13, and TNFR2 accurately distinguished patients with DM and ASS from healthy controls, as shown by the area under the curve >0.80. Moreover, receiver operating characteristic analysis showed that, as biomarkers for discrimination between DM and ASS, the combination of IL-1ra and TIMP-1, had an area under the curve of 0.944, a sensitivity of 87.5%, and a specificity of 88.9%.ConclusionOur study demonstrated that serum levels of cytokines/chemokines showed a different pattern in newly diagnosed patients with DM and ASS, in which serum IL-1ra and TIMP-1 could be used to distinguish between the two diseases.

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“…Pathview Web was used to generate biological pathway graphs 83, 84 . Cell-cell communication analysis was performed using Liana 136 and Celltalker 137 (https://github.com/arc85/celltalker). To accomplish this task, the counts and clusters data from Loupe Browser were imported into Seurat 138 .…”

Section: Methodsmentioning

confidence: 99%

A patterned human heart tube organoid model generated by pluripotent stem cell self-assembly

Volmert

Aguirre

Riggs

et al. 2022

Preprint

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Human pluripotent stem cells can recapitulate significant features of mammalian organ development in vitro, including key aspects of heart development. We hypothesized that the organoids thus created can be made substantially more relevant by mimicking aspects of in utero gestation, leading to higher physiological and anatomical resemblance to their in vivo counterparts. Here, we report steps towards generating developmentally inspired maturation methodologies to differentiate early human heart organoids into patterned heart-tube-like structures in a reproducible and high-throughput fashion by complete self-organization. The maturation strategy consists of the controlled and stepwise exposure to metabolic (glucose, fatty acids) and hormonal signals (T3, IGF-1) as present during early heart development. These conditions elicit important transcriptomic, cellular, morphological, metabolomic, and functional changes over a 10-day period consistent with continuously increasing heart complexity, maturation, and patterning. Our data reveals the emergence of atrial and ventricular cardiomyocyte populations, valvular cells, epicardial cells, proepicardial-derived cells, endothelial cells, stromal cells, conductance cells, and cardiac progenitors, all of them cell types present in the primitive heart tube. Anatomically, the organoids elongate and develop well-differentiated atrial and ventricular chambers with compacted myocardial muscle walls and a proepicardial organ. For the first time in a completely self-organizing heart organoid, we show anterior-posterior patterning due to an endogenous retinoic acid gradient originating at the atrial pole, where proepicardial and atrial populations reside, mimicking the developmental process present within the primitive heart tube. Collectively, these findings highlight the ability of self-organization and developmental maturation strategies to recapitulate human heart development. Our patterned human heart tube model constitutes a powerful in vitro tool for dissecting the role of different cell types and genes in human heart development, as well as disease modeling congenital heart defects, and represents a step forward in creating fully synthetic human hearts.

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The Expression of Cytokine Profiles and Related Receptors in Idiopathic Inflammatory Myopathies

Cited by 9 publications

References 45 publications

Associations of Serum CXCL12α and CK Levels with Skeletal Muscle Mass in Older Adults

Associations of Serum CXCL12α and CK Levels with Skeletal Muscle Mass in Older Adults

Comparison of cytokine/chemokine profiles between dermatomyositis and anti-synthetase syndrome

A patterned human heart tube organoid model generated by pluripotent stem cell self-assembly

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