The expression of estrogen receptors during the <i>Mytilus galloprovincialis</i> ovarian cycle

Agnese, Marisa; Rosati, Luciano; Prisco, Marina; Borzacchiello, Luigi; Abagnale, Ludovico; Andreuccetti, Piero

doi:10.1002/jez.2272

Cited by 8 publications

(3 citation statements)

References 60 publications

(101 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Natural variation in the oestrogen receptor-like physiological conditions could be based on annual and seasonal contexts, as already noted for M. edulis MeER2 expression (Ciocan et al 2010). Similarly, variable expression of oestrogen receptor-like genes was found during M. galloprovincialis ovarian cycle (Agnese et al 2019) and throughout larval development (Balbi et al 2016). In this case, the initial hypothesis of this experiment that the action of certain stressors could be affected by the reproductive cycle did not find confirmation, as we found no significant influence by the gametogenesis stage on the gene expression of oestrogen receptor-like response.…”

Section: Expression Analysis Of Oestrogen Receptor-like Genessupporting

confidence: 55%

Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7

Mincarelli

Chapman

Rotchell

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

Plastic pollution and changes in oceanic pH are both pressing environmental issues. Little emphasis, however, has been placed on the influence of sex and gametogenesis stage when investigating the effects of such stressors. Here, we examined histology and molecular biomarkers of blue mussels Mytilus edulis exposed for 7 days to a pH 7.7 scenario (− 0.4 units) in combination with environmentally relevant concentrations (0, 0.5 and 50 µg/L) of the endocrine disrupting plasticiser di-2-ethylhexyl phthalate (DEHP). Through a factorial design, we investigated the gametogenesis cycle and sex-related expression of genes involved in pH homeostasis, stress response and oestrogen receptor-like pathways after the exposure to the two environmental stressors. As expected, we found sex-related differences in the proportion of developing, mature and spawning gonads in histological sections. Male gonads also showed higher levels of the acid–base regulator CA2, but females had a higher expression of stress response-related genes (i.e. sod, cat, hsp70). We found a significant effect of DEHP on stress response-related gene expression that was dependent on the gametogenesis stage, but there was only a trend towards downregulation of CA2 in response to pH 7.7. In addition, differences in gene expression between males and females were most pronounced in experimental conditions containing DEHP and/or acidified pH but never the control, indicating that it is important to consider sex and gametogenesis stage when studying the response of mussels to diverse stressors.

show abstract

Section: Expression Analysis Of Oestrogen Receptor-like Genessupporting

confidence: 55%

Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7

Mincarelli

Chapman

Rotchell

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…The long-term exposure data suggest similar levels of downregulation regardless of the sex of mussels and paracetamol concentration and thus a more widespread effect. Several studies have documented the expression of estrogen receptors in different invertebrates (Keay and Thornton 2009;Jones et al 2017), in addition to mussels (Puinean et al 2006;Agnese et al 2019;Balbi et al 2019). However, it is also worth considering that ER2 might display natural variation during different stages of gametogenesis (Ciocan et al 2010); hence, further investigation is required.…”

Section: The Variation Of Mrna Expression Of Target Genes In Mussel Gonadsmentioning

confidence: 99%

Long-term exposure of marine mussels to paracetamol: is time a healer or a killer?

Koagouw

Stewart

Ciocan

2021

Environ Sci Pollut Res

View full text Add to dashboard Cite

Pharmaceuticals pose a major threat to the marine environment, and several studies have recently described their negative effects on marine organisms. Pharmaceutical compounds are constantly being released into aquatic ecosystems, and chronic exposure, even at low concentrations, may have a major impact on marine organisms. The purpose of the present study is to evaluate the biological changes induced by one of the most widely used pharmaceuticals-paracetamol-in the blue mussel Mytilus edulis, after a long-term exposure at environmentally relevant concentrations. We present our data alongside and in comparison with results from a previous short-term exposure, to demonstrate the significance of exposure period on the effects of paracetamol in adult blue mussels. After 24 days of laboratory exposure, seven potential target genes were selected to examine toxicological effects in mussels' gonads and possible disruptive effects on reproductive processes. The results show the modulation of some important reproduction-related genes: estrogen receptor-2 (ER2), vitelline envelope zona pellucida domain-9 (V9), and vitellogenin (VTG). Variations in mRNA expression of four other genes involved in apoptosis (HSP70, CASP8, BCL2, and FAS) are also highlighted. Histopathological alterations caused by paracetamol, together with neutral red retention time response in mussels' hemocytes, are presented herein. Overall, this study highlights the exacerbated effects of low concentration of paracetamol after chronic exposure, similar to the damage induced by higher concentrations in a short exposure scenario, thus emphasizing the importance of length of exposure period when studying the effects of this substance. Additionally, this study also discusses the potential of paracetamol to inflict several major changes in the reproductive system of mussels and thus possibly affect the survival of populations.

show abstract

“…The long term exposure data suggest similar levels of downregulation regardless of the sex of mussels and paracetamol concentration, thus a more widespread effect. Several studies have documented the expression of estrogen receptors in different invertebrates (Keay and Thornton, 2009;Jones et al, 2017), in addition to mussels (Puinean et al, 2006;Agnese et al, 2019;Balbi et al, 2019). However, it is also worth considering that ER2 might display natural variation during different stages of gametogenesis (Ciocan et al, 2010), hence further investigation is required.…”

Section: The Variation Of Mrna Expression Of Target Genes In Mussel Gmentioning

confidence: 99%

Long term exposure of marine mussels to paracetamol: is time a healer or a killer?

Koagouw

Stewart

Ciocan

2021

Preprint

View full text Add to dashboard Cite

Pharmaceuticals pose a major threat to the marine environment and several studies have recently described their negative effects on marine organisms. Pharmaceutical compounds are constantly being released into aquatic ecosystems and chronic exposure, even at low concentrations, may have impact on marine organisms. The purpose of the present study is to evaluate the biological changes induced by one of the most widely used pharmaceuticals – paracetamol – in the blue mussel Mytilus edulis, after a long-term exposure at environmentally relevant concentrations. We present our data alongside and in comparison with results from a previous short-term exposure, to demonstrate the significance of exposure period on the effects of paracetamol in adult blue mussels. After 24 days of laboratory exposure, seven potential target genes were selected to examine toxicological effects in mussels’ gonads and possible disruptive effects on reproductive processes. The results show the modulation of some important reproduction-related genes: estrogen receptor-2 (ER2), vitelline envelope zona pellucida domain-9 (V9) and vitellogenin (VTG). Variations in mRNA expression of four other genes involved in apoptosis (HSP70, CASP8, BCL2 and FAS) are also highlighted. Histopathological alterations caused by paracetamol, together with neutral red retention time response in mussels’ hemocytes, are presented herein. Overall, this study highlights the exacerbated effects of low concentration of paracetamol after chronic exposure, similar to the damage induced by higher concentrations in a short exposure scenario, thus emphasizing the importance of length of exposure period when studying the effects of this substance. Additionally, this study also discusses the potential of paracetamol to inflict several major changes on the reproductive system of mussels and thus possibly affect the survival of populations.

show abstract

The expression of estrogen receptors during the Mytilus galloprovincialis ovarian cycle

Cited by 8 publications

References 60 publications

Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7

Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7

Long-term exposure of marine mussels to paracetamol: is time a healer or a killer?

Long term exposure of marine mussels to paracetamol: is time a healer or a killer?

Contact Info

Product

Resources

About