The expression of hSR-B1 on platelets of patients with coronary artery disease (CAD)

Hamidpour, Mohsen; Bashash, Davood; Nehzati, Parisa; Abbasalizadeh, Mahnaz; Nikoogoftar, Mahin; Hamidpour, Rafie

doi:10.3233/ch-170311

Cited by 2 publications

(3 citation statements)

References 22 publications

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“…In this study, following co-culture of monocytes with platelets, we investigated the mRNA expression of CD36 , scavenger receptor class A (SRA) , and acyl-coenzyme A cholesterol acyltransferase (ACAT) in monocytes, which are among the key genes in lipid accumulation [22,23]. We also evaluated the gene expression changes that are expected to enhance the cholesterol efflux from foam cells, including ATP-binding cassette transporter A1 (ABCA1) , ATP-binding cassette transporter G1 (ABCG1) , peroxisome proliferator-activated receptor γ (PPAR γ) , liver X receptor-α (LXR- α ) , and scavenger receptor class B (SRB) [24,25,26]. Our results showed that platelets elevated the expression of mRNA of CD36, ACAT, ABCA1, SRB, and LXR- α.…”

Section: Resultsmentioning

confidence: 99%

Co-culture of platelets with monocytes induced M2 macrophage polarization and formation of foam cells: shedding light on the crucial role of platelets in monocyte differentiation

Mehrpouri

Bashash

Mohammadi

et al. 2019

Tjh

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Objective: Far beyond hemostasis and thrombosis, significant evidence has indicated the critical role of platelets in atherosclerosis. SDF-1 is among the pro-inflammatory chemokines that are increased in platelets of patients with coronary artery disease (CAD). The goal of the current work is to identify the in vitro effect of platelets from either CAD patients or healthy volunteers on the induction of macrophages and foam cells. Materials and Methods: The expression of SDF-1 on platelet surfaces in CAD patients and healthy volunteers was investigated using flow cytometry. We also evaluated the CXCR4/CXCR7 expression on monocytes from buffy coats of healthy volunteers. The effect of platelets from CAD patients and healthy volunteers on differentiation of monocytes and foam cell formation was evaluated using Oil Red O (ORO) staining. Flow cytometry and real-time PCR were also employed to evaluate surface markers and mRNA expression of genes involved in this process after co-culture of platelets with monocytes. Results: Monocytes in co-culture with platelets acquired a spindleshape appearance and ORO-positive lipid droplets. In addition, platelets could induce CD163 expression, as an important marker of M2 macrophage, and upregulate the mRNA expression of the SRB, CD36, ACAT, LXR-α , and ABCA1 genes in monocytes. Notably, platelets of CAD patients with higher expression of SDF-1, increased the expression of genes encoding SRB and CD36 as compared to platelets of healthy volunteers. Conclusion: Our results indicate that platelets from CAD patients could provoke monocyte differentiation into macrophages with an M2 phenotype, which in turn may participate in an atheroprotective process.

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Section: Resultsmentioning

confidence: 99%

Co-culture of platelets with monocytes induced M2 macrophage polarization and formation of foam cells: shedding light on the crucial role of platelets in monocyte differentiation

Mehrpouri

Bashash

Mohammadi

et al. 2019

Tjh

Self Cite

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“…Multiple lines of evidence indicated that CD36 and ACAT pave the way for the formation of foam cells through enhancing the capability of the cells to uptake platelets/ox-LDL and cholesterol esterification, respectively [22,23]. On the other hand, increased expression levels of SRB, LXR-α, and ABCA1 were also reported to be associated with the cholesterol efflux from macrophage foam cells [24,25,26]. Other studies also showed that the gene expression of proteins such as CD36, ACAT1, LXR-α, and ABCA1 are increased during macrophage-derived foam cell formation [22,23,31,32].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, following co-culture of monocytes with platelets, we investigated the mRNA expression of CD36, scavenger receptor class A (SRA), and acyl-coenzyme A cholesterol acyltransferase (ACAT) in monocytes, which are among the key genes in lipid accumulation [22,23]. We also evaluated the gene expression changes that are expected to enhance the cholesterol efflux from foam cells, including ATP-binding cassette transporter A1 (ABCA1), ATP-binding cassette transporter G1 (ABCG1), peroxisome proliferator-activated receptor γ (PPARγ), liver X receptor-α (LXR-α), and scavenger receptor class B (SRB) [24,25,26]. Our results showed that platelets elevated the expression of mRNA of CD36, ACAT, ABCA1, SRB, and LXR-α.…”

Section: Platelets Induced the Expression Of The Genes Involved In Chmentioning

confidence: 99%

Co-culture of Platelets with Monocytes Induced M2 Macrophage Polarization and Formation of Foam Cells: Shedding Light on the Crucial Role of Platelets in Monocyte Differentiation

Mehrpouri¹,

Bashash²,

Mohammadi³

et al. 2019

Tjh

Self Cite

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Far beyond hemostasis and thrombosis, significant evidence has indicated the critical role of platelets in atherosclerosis. SDF-1 is among the pro-inflammatory chemokines that are increased in platelets of patients with coronary artery disease (CAD). The goal of the current work is to identify the in vitro effect of platelets from either CAD patients or healthy volunteers on the induction of macrophages and foam cells. Materials and Methods: The expression of SDF-1 on platelet surfaces in CAD patients and healthy volunteers was investigated using flow cytometry. We also evaluated the CXCR4/CXCR7 expression on monocytes from buffy coats of healthy volunteers. The effect of platelets from CAD patients and healthy volunteers on differentiation of monocytes and foam cell formation was evaluated using Oil Red O (ORO) staining. Flow cytometry and real-time PCR were also employed to evaluate surface markers and mRNA expression of genes involved in this process after co-culture of platelets with monocytes. Results: Monocytes in co-culture with platelets acquired a spindleshape appearance and ORO-positive lipid droplets. In addition, platelets could induce CD163 expression, as an important marker of M2 macrophage, and upregulate the mRNA expression of the SRB, CD36, ACAT, LXR-α, and ABCA1 genes in monocytes. Notably, platelets of CAD patients with higher expression of SDF-1, increased the expression of genes encoding SRB and CD36 as compared to platelets of healthy volunteers. Conclusion: Our results indicate that platelets from CAD patients could provoke monocyte differentiation into macrophages with an M2 phenotype, which in turn may participate in an atheroprotective process.

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The expression of hSR-B1 on platelets of patients with coronary artery disease (CAD)

Abstract: Taken together, the results of the present study raise the possibility that the measurement of hSR-B1 expression on human platelets may provide a valuable insight that reflects the status of RCT in patients with atherosclerosis.

Cited by 2 publications

References 22 publications

Co-culture of platelets with monocytes induced M2 macrophage polarization and formation of foam cells: shedding light on the crucial role of platelets in monocyte differentiation

Co-culture of platelets with monocytes induced M2 macrophage polarization and formation of foam cells: shedding light on the crucial role of platelets in monocyte differentiation

Co-culture of Platelets with Monocytes Induced M2 Macrophage Polarization and Formation of Foam Cells: Shedding Light on the Crucial Role of Platelets in Monocyte Differentiation

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