The Expression of Matrix Metalloproteinases in Eyes with Intraocular Lymphoma

Fukutsu, Kanae; Kase, Satoru; Iwata, Daiju; Suzuki, Kayo; Namba, Kenichi; Ishida, Susumu

doi:10.1080/09273948.2021.1957122

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…The enzyme encoded by this gene degrades type IV and V collagens. Studies suggest a role in tumor-associated tissue remodeling 193 .…”

Section: Evaluating Htmc On Se Scaffoldmentioning

confidence: 99%

Mechanical and morphological modulation of electrospun polymeric scaffolds for tissue engineering applications

Bikuña

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Electrospinning technologies herald the arrival of a new era in which previously unthinkable scaffolds for tissue engineering applications will be solved efficiently. However, electrospinning techniques, like solution electrospinning and melt electrowriting are held down by fabrications parameters, technology limitations, and the application perse. The science of scaffolding fabrication seeks to mimic the extracellular matrix of a particular tissue in ways that mitigates the damage or enables its pathophysiological study. Thenceforce, scaffolds have the primordial role of not only supporting the cells, but to replicate as close as possible the native extracellular matrix, taking into consideration the biocompatibility, biodegradability, morphology and mechanical properties. The last two properties are pivotal in the scaffold ́s outcome, as cells communicate with the environment, and behave in response to external signals. In context of scaffolds ́ assembly, electrospinning fabrication parameters should be correctly modulated, to ensure an appropriate cellular environment. In this dissertation we attempt to tackle this concern relying on solution electrospinning and melt electrowriting techniques. As potential tissue engineering applications, the recreation of an artificial human trabecular meshwork and a skeletal muscle platform are developed. The mechanical and morphological requirements of each tissue are evaluated and fabrication parameters adapted. An in vitro human trabecular meshwork scaffold was developed and validated with human trabecular meshwork cells ́ behavioral studies. With the development of a perfusion bioreactor human trabecular meshwork cells react to medicaments inducing measurable pressure changes. Finally, an attempt for skeletal muscle platform was made. This first approach enabled us the optimization of the process for next attempts.

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“…The enzyme encoded by this gene degrades type IV and V collagens. Studies suggest a role in tumor-associated tissue remodeling 193 .…”

Section: Evaluating Htmc On Se Scaffoldmentioning

confidence: 99%

Mechanical and morphological modulation of electrospun polymeric scaffolds for tissue engineering applications

Bikuña

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Clinical significance of serum and vitreous soluble interleukin-2 receptor in patients with intraocular lymphoma

et al. 2022

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Background Intraocular lymphoma (IOL) is a masquerade syndrome that mimics uveitis, making diagnosis difficult. The serum soluble interleukin-2 receptor (sIL-2R), which is cleaved by matrix metalloproteinase (MMP) -2 and MMP-9, has been recognized as a tumor-related biomarker of malignant lymphomas. The aim of this study was to review the reliability of serum and vitreous sIL-2R for distinguishing IOL from uveitis. Methods Patients who underwent diagnostic vitrectomy for marked vitreous haze at Hokkaido University Hospital between April 2014 and June 2019 were enrolled. The patients were divided into an IOL group and a uveitis group, according to the pathology of their vitreous samples. The IOL group was further divided at the time of vitrectomy into patients who already had extraocular involvement (IOL with extraocular involvement group) and patients with no evidence of having extraocular involvement (IOL without extraocular involvement group). Serum sIL-2R, and intravitreal sIL-2R, MMP-2, and MMP-9 levels were assessed. Results Twenty-five eyes of 25 patients, and 15 eyes of 15 patients were included in the IOL group and uveitis group, respectively. The serum sIL-2R levels were significantly lower in the IOL group than in the uveitis group (P < 0.05), and 20.0% and 66.7% in the IOL and the uveitis group showed high sIL-2R value above the normal range. Vitreous sIL-2R tended to be higher in the IOL group than in the uveitis group (P = 0.80). Serum sIL-2R was significantly lower in the IOL without extraocular involvement group than in the IOL with extraocular involvement group (P < 0.05); 5.9% in the IOL without extraocular involvement group and 50.0% in the IOL with extraocular involvement group showed high sIL-2R value above the normal range. Vitreous sIL-2R, MMP-2, and MMP-9 tended to be higher in the IOL with extraocular involvement group than in the IOL without extraocular involvement group (P = 0.30, < 0.05, 0.16). Conclusions Serum sIL-2R is often within the normal range in IOL patients. Even if it is within the normal range, the possibility of IOL should be considered. Serum sIL-2R is not a reliable biomarker for IOL, whereas vitreous sIL-2R may be useful for the diagnosis of IOL.

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The Expression of Matrix Metalloproteinases in Eyes with Intraocular Lymphoma

Cited by 2 publications

References 12 publications

Mechanical and morphological modulation of electrospun polymeric scaffolds for tissue engineering applications

Mechanical and morphological modulation of electrospun polymeric scaffolds for tissue engineering applications

Clinical significance of serum and vitreous soluble interleukin-2 receptor in patients with intraocular lymphoma

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