The expression of miR-25 is increased in colorectal cancer and is associated with patient prognosis

Abstract

MicroRNA-25 (miR-25) has recently been found to be involved in many critical processes in human malignancies. We aimed to investigate the expression pattern and prognostic role of miR-25 in colorectal cancer. Colorectal cancer and adjacent normal specimens from 186 patients who had not received neoadjuvant chemotherapy were collected. The expression of miR-25 was detected with a quantitative real-time PCR assay, and the association of miR-25 with overall patient survival was analyzed via statistical analysis. The results indicated that the level of miR-25 expression was significantly elevated in colorectal cancer compared with the level observed in the adjacent normal tissue. It was also demonstrated that miR-25 expression is associated with tumor invasion, lymph node metastasis, distant metastasis and the TNM stage of colorectal cancer. In addition, a Kaplan-Meier analysis revealed that an increased level of miR-25 expression is associated with a poor overall survival of patients. A multivariate survival analysis also indicated that miR-25 is an independent prognostic marker after adjusting for known prognostic factors. These results prove that miR-25 expression is increased in colorectal cancer and is associated with tumor progression. This study also provides the first evidence that miR-25 is an independent prognostic factor for patients with colorectal cancer, indicating the potential role of miR-25 as a highly specific and sensitive biomarker.

miR-25 promotes glioblastoma cell proliferation and invasion by directly targeting NEFL

“…In ovarian cancer, miR-25 promotes cell proliferation and motility by targeting LATS2 [18]. Upregulation of miRNA-25 in colorectal cancer and hepatocellular carcinoma was associated with patient poor prognosis [20,29]. The present profile data further confirmed an increased expression of miR-25 both in astrocytoma control.…”

“…Due to their small size and influence in a broad range of biological processes, miRNAs are very attractive therapeutic targets for GBM. miR-25 is one of the most overexpressed miRNAs in a number of profiling experiments designed for the detection of miRNAs dysregulated in human cancers [16][17][18][19][20][21][22][23][24][25]29]. Li et al reported that miR-25 promoted gastric cancer migration, invasion, and proliferation by directly targeting transducer of ERBB2 and 1 and correlated with poor survival [17].…”

“…[16][17][18][19][20][21][22][23][24][25]. However, the role of miR-25 in glioblastoma tumorigenesis and the underlying molecular mechanisms by which miR-25 exerts its functions had remained-until recently-largely unknown.…”

Inhibition of microRNA-25 by tumor necrosis factor α is critical in the modulation of vascular smooth muscle cell proliferation

“…miR-25 is known to be important in several types of cancer, including hepatocellular carcinoma, and colon, gastric and lung cancer (34)(35)(36). miR-25 has been indicated to be involved in various cellular processes, including cell proliferation, apoptosis and the release of cytokines (37)(38)(39)(40). Esposito et al (36) demonstrated that miR-25 was reduced in anaplastic thyroid carcinomas, and ectopic expression of miR-25 suppressed the proliferation and colony formation of anaplastic thyroid carcinoma cells via the induction of G 2 /M-phase cell cycle arrest (36).…”

Tissue micro-RNAs associated with colorectal cancer prognosis: a systematic review

“…Two studies demonstrated the upregulation of miR-182 43, 44 associated with advanced TNM stage and others 22 studies pointed 23 different upregulated miRs associated with advanced TNM stage. In detail, miR-1296 84 , miR-888 64 , miR-21 29 , miR-130a 85 , miR-7 55 , miR-96 86 , miR-20a-5p 63 , miR-517a 87 , miR-106b 88 , miR-320e 89 , miR-592 90 , miR-196a 57 , miR-196b 57 , miR-720 91 , miR-133a 42 , miR-630 92 , miR-181a 31 , miR-210 93 , miR-25 94 , miR-191 95 , miR-92a 96 , miR-31 59 and miR-552 97 have been reported.…”