The expression of nectin-1alpha in normal human skin and various skin tumours

Matsushima, Hironori; Utani, Atsushi; Endo, Hisayoshi; Matsuura, Hiroshi; Kakuta, Mari; Nakamura, Yuumi; Matsuyoshi, Norihisa; Matsui, Chieko; Nakanishi, Hiroyuki; Takai, Yoshimi; Shinkai, Hiroshi

doi:10.1046/j.1365-2133.2003.05225.x

Cited by 39 publications

(28 citation statements)

References 36 publications

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“…Nectin-1 is expressed at cell-cell junctions in human and mouse epidermis (Matsushima et al, 2003;Wakamatsu et al, 2007). In mice lacking the gene encoding nectin-1 (Pvrl1 2/2 mice), the expression of loricrin, a differentiation marker and a major component of cornified cell envelopes in the epidermis, is downregulated and newborn pups have a shiny and slightly reddish skin (Wakamatsu et al, 2007).…”

Section: Cell Differentiationmentioning

confidence: 99%

The role of nectins in different types of cell–cell adhesion

Rikitake

Mandai

Takai

2012

Journal of Cell Science

141

121

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SummaryMammalian tissues and organs are composed of different types of cells that adhere to each other homotypically (i.e. interactions between cells of the same cell type) or heterotypically (i.e. interactions between different cell types), forming a variety of cellular patterns, including mosaic patterns. At least three types of cell-cell adhesion have been observed: symmetric homotypic, asymmetric homotypic and heterotypic cell adhesions. Cadherins and nectins, which are known cell-cell adhesion molecules, mediate these cell adhesions. Cadherins comprise a family of more than 100 members, but they are primarily involved in homophilic trans-interactions (i.e. interactions between the same cadherin members) between opposing cells. By contrast, the nectin family comprises only four members, and these proteins form both homophilic and heterophilic trans-interactions (i.e. interactions between the same and different nectin members on opposing cells). In addition, heterophilic trans-interactions between nectins are much stronger than homophilic trans-interactions. Because of these unique properties, nectins have crucial roles in asymmetric homotypic cell-cell adhesion at neuronal synapses and in various types of heterotypic cell-cell adhesions. We summarize recent progress in our understanding of the biology of nectins and discuss their roles in heterotypic cell-cell adhesions, whose formation cannot be solely explained by the action of cadherins.

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Section: Cell Differentiationmentioning

confidence: 99%

The role of nectins in different types of cell–cell adhesion

Rikitake

Mandai

Takai

2012

Journal of Cell Science

141

121

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“…Expression of nectin-1 has been previously localized in the dental epithelium during tooth morphogenesis. At the stage of tooth initiation, nectin-1 mRNA is expressed in suprabasal epithelium and during advanced morphogenesis in the SI cells deriving from the suprabasal epithelium (Matsushima et al, 2003;Okabe et al, 2004;Laurikkala et al, 2006). We compared the expression patterns of nectin-1 and -3 in the maxillary molars at the bud stage as well as during later stages of morphogenesis and cell differentiation by in situ hybridization (ISH).…”

Section: Nectin-1 and Nectin-3 Are Expressed In Overlapping Patterns mentioning

confidence: 99%

Cooperation of nectin‐1 and nectin‐3 is required for normal ameloblast function and crown shape development in mouse teeth

Yoshida

Miyoshi

Takai

et al. 2010

Developmental Dynamics

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Nectins are immunoglobulin-like cell adhesion proteins and their interactions recruit various cell-cell junctions. Mutations in human NECTIN-1 cause an ectodermal dysplasia syndrome, but Nectin-1 null mice have only slight defects in teeth, suggesting compensation by other nectin(s). We observed overlapping expression of nectin-3 with nectin-1 and enamel abnormality in the nectin-3 mutant. We, therefore, generated nectin-1;nectin-3 compound mutants. However, all teeth developed and no significant dental abnormalities were observed before birth. At postnatal day 10, the upper molars of compound mutants exhibited conical crown shape and retarded enamel maturation. Nectin-1 was expressed in ameloblasts whereas nectin-3 was expressed in neighboring stratum intermedium cells at this stage. The immunohistochemical localization and electron microscopical observations indicated that the desmosomal junctions between stratum intermedium and ameloblasts were significantly reduced. These results suggest that heterophilic interaction between nectin-1 and nectin-3 recruits desmosomal junctions, and that these are required for proper enamel formation. Developmental Dynamics 239:2558-2569,

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“…We have shown that normal human epidermis expresses nectin-1 at the cell-cell junctions and that nectin-1 colocalizes with E-cadherin there (25). The expression of nectin-1 at cellcell junctions was reduced in human epithelial cancer cells located at the advancing border of the tumor, losing the cell-cell junctions and facilitating the invasion of cancer cells into the neighboring tissue.…”

mentioning

confidence: 92%

“…Antibodies-A rabbit anti-nectin-1 polyclonal antibody (pAb), which recognizes the intracellular region of both mouse and human nectin-1, was prepared as described (25,29). Rat anti-nectin-1 and rat anti-nectin-2 monoclonal antibodies (mAbs), both of which recognize the extracellular regions of nectin-1 and nectin-2, respectively, were prepared as described (18,29).…”

Section: Generation Of Nectin-1mentioning

confidence: 99%

Up-regulation of Loricrin Expression by Cell Adhesion Molecule Nectin-1 through Rap1-ERK Signaling in Keratinocytes

Wakamatsu

Ogita

Okabe

et al. 2007

Journal of Biological Chemistry

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Nectin is an immunoglobulin-like cell-cell adhesion molecule, which plays essential roles in the initial step of formation of adherens junctions and tight junctions. We demonstrate here the role of nectin-1 in the epidermis using nectin-1 ؊/؊ mice. Newborn nectin-1 ؊/؊ pups showed shiny and slightly reddish skin; the amount of loricrin, one of the differentiation markers and also a major component of cornified cell envelopes, was markedly reduced in the epidermis of nectin-1 ؊/؊ mice. The amounts of repetin and SPRRP, other components of cornified cell envelopes, were markedly elevated probably due to a compensatory mechanism to overcome the impaired expression of loricrin. However, cornified cells from nectin-1 ؊/؊ mice were sensitive to mechanical stress. Moreover, Ca 2؉ -induced activation of ERK through Rap1 and expression of loricrin were reduced in primary cultured nectin-1 ؊/؊ keratinocytes; in turn, the inhibition of ERK activation reduced the amount of loricrin in wild-type keratinocytes. These results indicate that nectin-1 plays a key role in the expression of loricrin in the epidermis.

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The expression of nectin-1alpha in normal human skin and various skin tumours

Abstract: Our results showed that nectin-1alpha is located at cell-cell adherens junctions in human skin and that reduction of nectin-1alpha at cell-cell adherens junctions may be involved in the invasion of squamous cell tumours.

Cited by 39 publications

References 36 publications

The role of nectins in different types of cell–cell adhesion

The role of nectins in different types of cell–cell adhesion

Cooperation of nectin‐1 and nectin‐3 is required for normal ameloblast function and crown shape development in mouse teeth

Up-regulation of Loricrin Expression by Cell Adhesion Molecule Nectin-1 through Rap1-ERK Signaling in Keratinocytes

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