The Expression of Nestin in the Induced Differentiation Into Neurons of Rat Bone Marrow Mesenchymal Stem Cells by Neurotrophin-3 (Nt-3)

Noviantari, Ariyani; Antarianto, Radiana Dhewayani; Rif’ati, Lutfah; Rinendyaputri, Ratih; Zainuri, Masagus; Dany, Frans

doi:10.22159/ijap.2020.v12s3.39472

Cited by 3 publications

(5 citation statements)

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“…The administration of neurotrophins is a potential approach to the therapy of neurodegenerative disorders or injuries such as Parkinson's disease (PD), Alzheimer's disease (AZ), spinal cord, brain trauma, and brain tumors [31]. reported that MSCs can be differentiated into neurons with a medium containing NT-3 as a single neurotrophic factor and expressed nestin in the immature neural differentiation of MSCs [20]. Thus, in this study, NT-3 was used to investigate the expression of MAP-2 in the mature neural differentiation of MSCs with a single neurotrophic factor.…”

Section: Discussionmentioning

confidence: 99%

“…showed that the addition of bFGF, EGF, and BDNF to the medium induced the differentiation of rat bone marrow MSCs into neurons and glia and characterized neural markers like A2B5, beta-III-tubulin, PSA-NCAM, and MAP-2) [19]. also reported that NT-3 role as a single neurotrophic factor in the expression of nestin in the immature neural differentiation of MSCs [20]. A combination of growth factors and neurotrophic factors consistently demonstrated efficacy in neural differentiation protocols.…”

Section: Introductionmentioning

confidence: 92%

“…Rat MSCs were harvested by trypsinization using trypsin ethylenediaminetetraacetic acid (EDTA) and plated in a 24-well plate with a cell concentration of 2 × 10 4 /mL. The neural induction and differentiation techniques of MSCs were altered from those of [20]. The cells were cultured in an induction medium (MEM) with 1% N-2 supplements, 2% FBS, and NT-3 with concentrations of 20, 25, or 30 ng/mL in each well.…”

Section: Neuronal Induction and Differentiationmentioning

confidence: 99%

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Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

Noviantari¹,

Antarianto²,

Rif’ati³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Mesenchymal stem cells (MSCs) can differentiate into neurons, which can be used in cell therapy. Endogenous genes and neurotrophic factors such as nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 regulate neural stem cell proliferation and differentiation (NT-3). The differentiation of MSCs into neurons is characterized by the development of neurons that interact with each other to form rings or net-like structures. The markers of neurons in the immature stage are Nestin and Vimentin. While the markers for neurons at the mature stage are neuron-specific enolase (NSE), neuronalspecific nuclear protein (NeuN), and microtubule-associated protein-2 (MAP-2). This research aims to investigate the expression of MAP-2 on the differentiation of MSCs into mature neurons. MSCs were isolated from rat bone marrow of femur and tibia using flushing methods, then cultured in Minimum Essential Medium Eagle (MEM), 10% Fetal Bovine Serum (FBS), and 1% antibiotic-antimycotic. Neuron differentiation induction medium (MEM, 2% FBS, 1% insulin-like growth factor (N2), and NT-3 20, 25, and 30 ng/mL) was used to stimulate MSCs for 14 days (control induction medium without NT-3). The immunocytochemistry of MAP-2 was performed on day 14. All experiments were done triplicated. The data obtained is the average percentage of the number of MAP-2 positive cells at each concentration. SPSS statistical analysis with a one-way ANOVA test. The results showed a significant difference between the percentage of MAP-2 positive cells at NT-3 concentrations 20 (p < 0.05), 25 (p = 0,093), 30 ng/mL (p = 0,081), and the negative control. At concentrations of NT-3 20, 25, and 30 ng/mL the

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Neuronal Induction and Differentiationmentioning

confidence: 99%

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Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

Noviantari¹,

Antarianto²,

Rif’ati³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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“…The cells were passaged at 70% to 80% confluency [20]. The stem cells were previously confirmed by Ghorbanian et al (2012) [21,22].…”

Section: Rbmscs Isolation and Culturementioning

confidence: 94%

Sinusoidal Electromagnetic Field Decreases Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

ALTEMEMY,

GHASEM KASHANI,

N. WENAS

2024

Int J App Pharm

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Objective: The widespread use of household electrical appliances generating electric and magnetic fields was a significant focus of WHO attention because of its serious threat to human health, especially osteogenesis. This research investigated the effect of 50 Hz frequency (1 mT intensity) sinusoidal EMF (SEMF) on the osteogenic differentiation of rat bone marrow stem cells (rBMSCs) in vitro. Methods: Experimental groups were: positive control (cells cultured in osteogenic medium supplemented with 7-10 M Dexamethasone, negative control (cells cultured in α-MEM/10% FBS, 10 mmol Beta-Glycerol-Phosphate, 15% FBS, 50 ug/ml Ascorbic Acid bi-Phosphate, 100 unit/ml Penicillin) and for the EMF group, cells exposed to SEMF (50 Hz, 1 mT, 30 min/day) for 14 and 21 d. Alizarin red staining, Alkaline phosphatase activity, and QRT-PCR were performed. Results: The EMF group exhibited weaker positive stains for ALP and Alizarin red than the positive control group. The Runx2 and Ocn gene expression levels were significantly decreased compared to negative control at 14 and 21 d of EMF exposure, respectively. After 14 and 21 d of exposure, Runx2 and Ocn gene expression were much lower in the EMF group than in the positive control group. Conclusion: SEMF (1 mT, 50 Hz, 30 min/day) could retarded osteogenesis and reduce the osteogenic differentiation of rBMSCs.

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“…Keterangan: Kontrol negatif (A), Kontrol positif (B), SPM dalam medium yang ditambah NT-3 hari ke-7 (C), dan hari ke-14 (D). warna coklat menunjukkan sel positif Nestin (perbesaran 400x) 20 dari sumsum tulang tikus dapat berdiferensiasi menjadi neuron dengan penambahan NT-3 dalam medium kultur. Perkembangan neuron diamati dengan ekspresi penanda Nestin pada hari ke-7 dan ke-14 (Gambar 4).…”

Section: Gambar 4 Ekspresi Nestin Pada Diferensiasi Spmunclassified

Kajian: Aplikasi Sel Punca Mesenkim pada Tata Laksana Klinis Penyakit Stroke

Noviantari

Febrianti

2021

mpk

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Stroke is a neurodegenerative disease that can cause disability to death. Stroke therapy useing stem cells is starting to be considered as an alternative in terms of availability, propagation potential, and differentiation ability. Stem cells are cells that have the ability to reproduce (self-renewal), do not yet have a specific shape and functions (undifferentiated), but have the ability todifferentiate into other cells. One type of stem cell is mesenchymal stem cell (MSC) which can differentiate in vitro into other cells including neurons. In addition MSC can secrete various neurotrophic factors that can play a role in angiogenesis and synaptic formation, so that, it is relevant to stroke therapy. This paper describes the research and development of stem cell applications in stroke therapy through several research results. This paper is in the form of a literature review obtained through literature searches. The results showed that MSC is able to differentiate and replace damaged neurons after transplantation based on the results of studies in vitro, in vivo, and several clinical trials. The technique in the differentiation of MSCinto neurons is the addition of certain growth factors to the culture medium in vitro. Meanwhile, in vivo studies have shown that that administration of MSC can replace damaged cells, provide neuroprotective effect, induce axon growth, stimulate angiogenesis, and neurogenesis in stroke animal models using the middle cerebral artery occlusion (MCAO) method. Therefore, MSCs have the potential to treat degenerative diseases, especially stroke. From the results of clinical trials, it can be seen that stem cells are safe to give to stroke patients, there is clinical improvement, and neurological improvement, as well as a reduction in brain lesions. However, there are several challenges for the development and application of MSS for stroke therapy in the future such as concerns about tumorigenesis and high cost. Abstrak Stroke adalah penyakit neurodegeneratif yang dapat menyebabkan kecacatan hingga kematian. Terapi stroke menggunakan sel punca mulai dipikirkan sebagai alternatif dilihat dari aspek ketersediaan, potensi perbanyakan, dan kemampuan diferensiasinya. Sel punca merupakan sel yang memiliki kemampuan memperbanyak diri (self renewal), belum mempunyai bentuk dan fungsi yang spesifik (undifferentiated), namun memiliki kemampuan untuk berdiferensiasi menjadi sel lainnya. Salah satu jenis sel punca adalah sel punca mesenkim (SPM) yang dapat berdiferensiasi secara in vitro menjadi sel lain termasuk neuron. Selain itu, SPM dapat menyekresikan berbagai faktor neurotrofik yang dapat berperan dalam angiogenesis dan pembentukan sinaptik sehingga relevan terhadap terapi terhadap stroke. Tulisan ini menguraikan tentang penelitian dan pengembangan sel punca dalam terapi stroke melalui beberapa hasil penelitian. Tulisan ini berupa review literatur yang didapatkan melalui penelusuran pustaka. Hasilnya menunjukkan bahwa SPM mampu berdiferensiasi dan menggantikan neuron yang rusak setelah transplantasi berdasarkan hasil penelitian secara in vitro, in vivo, dan beberapa uji klinis. Teknik dalam diferensiasi SPM menjadi neuron adalah dengan penambahan faktor-faktor pertumbuhan tertentu ke dalam medium kultur secara in vitro. Sedangkan studi secara in vivo menunjukkan bahwa pemberian SPM dapat menggantikan sel yang rusak, memberi efek neuroprotektif, menginduksi pertumbuhan akson, menstimulasi angiogenesis, dan neurogenesis pada hewan model stroke dengan metode middle cerebral artery occlusion (MCAO). Oleh sebab itu, sel punca mesenkim berpotensi dalam terapi penyakit degeneratif terutama stroke. Dari hasil uji klinis terlihat bahwa sel punca aman diberikan kepada pasien stroke, terjadi perbaikan klinis, dan perbaikan neurologis, serta terjadi pengurangan lesi di otak. Namun, terdapat beberapa tantangan untuk pengembangan dan aplikasi SPM untuk terapi stroke dimasa mendatang, seperti kekhawatiran terjadinya tumorigenesis dan biaya yang cukup mahal.

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The Expression of Nestin in the Induced Differentiation Into Neurons of Rat Bone Marrow Mesenchymal Stem Cells by Neurotrophin-3 (Nt-3)

Cited by 3 publications

References 20 publications

Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

Immunocytochemistry Studies Using Microtubule-Associated Protein-2 (MAP-2) Markers on Neural Differentiation of Mesenchymal Stem Cells from Rat Bone Marrow

Sinusoidal Electromagnetic Field Decreases Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

Kajian: Aplikasi Sel Punca Mesenkim pada Tata Laksana Klinis Penyakit Stroke

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