The expression of peroxisomal protein transcripts increased by retinoic acid during neural differentiation☆

Ostadsharif, Maryam; Ghaedi, Kamran; Nasr‐Esfahani, Mohammad Hossein; Mojbafan, Marzieh; Tanhaie, Somayeh; Karbalaie, Khadijeh; Baharvand, Hossein

doi:10.1016/j.diff.2010.11.003

Cited by 39 publications

(29 citation statements)

References 16 publications

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“…Previous studies showed that Fndc5 gene expression is induced by ATRA treatment during neural differentiation of embryonic carcinoma cells (P19) and mouse embryonic stem cells [30, 49]. ATRA effects on Fndc5 gene transcription could be direct or indirect and mechanistic details remain to be established.…”

Section: Discussionmentioning

confidence: 99%

Retinoic Acid Increases Fatty Acid Oxidation and Irisin Expression in Skeletal Muscle Cells and Impacts Irisin In Vivo

Amengual

García-Carrizo

Arreguín

et al. 2018

Cell Physiol Biochem

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Background/Aims: All-trans retinoic acid (ATRA) has protective effects against obesity and metabolic syndrome. We here aimed to gain further insight into the interaction of ATRA with skeletal muscle metabolism and secretory activity as important players in metabolic health. Methods: Cultured murine C2C12 myocytes were used to study direct effects of ATRA on cellular fatty acid oxidation (FAO) rate (using radioactively-labelled palmitate), glucose uptake (using radioactively-labelled 2-deoxy-D-glucose), triacylglycerol levels (by an enzymatic method), and the expression of genes related to FAO and glucose utilization (by RT-real time PCR). We also studied selected myokine production (using ELISA and immunohistochemistry) in ATRA-treated myocytes and intact mice. Results: Exposure of C2C12 myocytes to ATRA led to increased fatty acid consumption and decreased cellular triacylglycerol levels without affecting glucose uptake, and induced the expression of the myokine irisin at the mRNA and secreted protein level in a dose-response manner. ATRA stimulatory effects on FAO-related genes and the Fndc5 gene (encoding irisin) were reproduced by agonists of peroxisome proliferator-activated receptor β/δ and retinoid X receptors, but not of retinoic acid receptors, and were partially blocked by an AMP-dependent protein kinase inhibitor. Circulating irisin levels were increased by 5-fold in ATRA-treated mice, linked to increased Fndc5 transcription in liver and adipose tissues, rather than skeletal muscle. Immunohistochemistry analysis of FNDC5 suggested that ATRA treatment enhances the release of FNDC5/irisin from skeletal muscle and the liver and its accumulation in interscapular brown and inguinal white adipose depots. Conclusion: These results provide new mechanistic insights on how ATRA globally stimulates FAO and enhances irisin secretion, thereby contributing to leaning effects and improved metabolic status.

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Section: Discussionmentioning

confidence: 99%

Retinoic Acid Increases Fatty Acid Oxidation and Irisin Expression in Skeletal Muscle Cells and Impacts Irisin In Vivo

Amengual

García-Carrizo

Arreguín

et al. 2018

Cell Physiol Biochem

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“…In this context, it is interesting to note that retinoic acid was reported to modulate the expression of the newly identified peroxisomal protein PeP in mouse embryonic stem cells, proposing a third member of the nuclear receptor superfamily with an impact on peroxisome function (Ostadsharif et al 2011). H 2 O 2 , which is produced by the numerous peroxisomal oxidases, is supposed to act as an intracellular signaling molecule modulating proteins by selective oxidation of cysteine residues (see ''Mysterious signals: peroxisomes and reactive oxygen species'').…”

Section: Mysterious Proliferation: Ppara and Beyond?mentioning

confidence: 99%

The peroxisome: an update on mysteries

Islinger

Grille

Fahimi

et al. 2012

Histochem Cell Biol

196

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“…The medium was changed everyday and cells were passaged every 2 days. Neural differentiation was carried out according to the previously described protocol through embryoid body (EB) formation in hanging drops for 2 days (Ostadsharif et al 2011). Subsequently, EBs were plated on gelatin-coated 12-well plates (TPP, Switzerland) for an additional 6 days in a neurobasal medium (Invitrogen) that contained 5% ES-FCSand penicillin-streptomycin supplemented with 0.1 mM non-essential amino acids, 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, and 2% B27 supplement (Invitrogen).…”

Section: Mescs Culture and Neural Differentiation Stagesmentioning

confidence: 99%

“…We previously showed that expression level of Fndc5 was increased upon treating with retinoic acid (RA) during neural differentiation of mESCs, suggesting a possible role in neural differentiation (Ostadsharif et al 2011). Furthermore, our group demonstrated that Fndc5 knockdown significantly decreased neural differentiation of mESCs (Hashemi et al 2013).…”

Section: Introductionmentioning

confidence: 97%

Zinc finger protein 521 overexpression increased transcript levels of Fndc5 in mouse embryonic stem cells

et al. 2016

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Zinc finger protein 521 is highly expressed in brain, neural stem cells and early progenitors of the human hematopoietic cells. Zfp521 triggers the cascade of neurogenesis in mouse embryonic stem cells through inducing expression of the early neuroectodermal genes Sox1, Sox3 and Pax6. Fndc5, a precursor of Irisin has inducing effects on the expression level of brain derived neurotrophic factor in hippocampus. Therefore, it is most likely that Fndc5 may play an important role in neural differentiation. To exhibit whether the expression of this protein is under regulation with Zfp521, we overexpressed Zfp521 in a stable transformants of mESCs expressing EGFP under control of Fndc5 promoter. Increased expression of Zfp521 enhanced transcription levels of both EGFP and endogenous Fndc5. This result was confirmed by overexpression the aforementioned vectors in HEK cells and indicated that Zfp521 functions upstream of Fndc5 expression. It is most likely that Zfp521 may act through the binding to its response element on Fndc5 core promoter. Therefore it is concluding that an enhanced expression of Fndc5 in neural progenitor cells is stimulated by Zfp521 overexpression in these cells.

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The expression of peroxisomal protein transcripts increased by retinoic acid during neural differentiation☆

Cited by 39 publications

References 16 publications

Retinoic Acid Increases Fatty Acid Oxidation and Irisin Expression in Skeletal Muscle Cells and Impacts Irisin In Vivo

Retinoic Acid Increases Fatty Acid Oxidation and Irisin Expression in Skeletal Muscle Cells and Impacts Irisin In Vivo

The peroxisome: an update on mysteries

Zinc finger protein 521 overexpression increased transcript levels of Fndc5 in mouse embryonic stem cells

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