The expression of placental-type glutathione S-tranferase (GST-?) in human cutaneous squamous cell carcinoma and normal human skin

Shimizu, Kazuhiro; Toriyama, Fumi; Yoshida, Hiroyuki

doi:10.1007/bf00199348

Cited by 17 publications

(12 citation statements)

References 22 publications

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“…The present study shows that a group of phase 2 enzymes are up-regulated in relation to the degree of cell diVerentiation in normal KC or SCC. Consistently, GST was reported to be expressed gradually from the supra-basal to granular layers of normal epidermis, and is expressed in cutaneous SCC, indicating diVerentiation-dependent expression in the skin [23]. These data, along with the results of the present study, suggest that the expression level of phase 2 enzymes in SCC is correlated with diVerentiation status, rather than the cellular redox status, of tumor cells.…”

Section: Discussionsupporting

confidence: 87%

Nrf2-dependent and Nrf2-independent induction of phase 2 detoxifying and antioxidant enzymes during keratinocyte differentiation

et al. 2012

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As antioxidant enzymes can be actively modulated during keratinocyte (KC) differentiation, this study was aimed to evaluate the modulation of a group of phase 2 detoxifying and antioxidant enzymes (phase 2 enzymes) during KC differentiation. In postconfluence-induced differentiation model of KC, heme oxygenase-1 (HO-1), NADP(H):quinone oxidoreductase-1 (NQO-1), and glutathione S-transferase pi (GSTpi) were up-regulated at a transcriptional level. In Western blot analysis, the phase 2 enzymes were up-regulated by H(2)O(2), but down-regulated by N-acetyl cysteine, indicating the active role of reactive oxygen species for their expression during KC differentiation. When a redox-sensitive NF-E2 related factor-2 (Nrf2), a key transcriptional factor for phase 2 enzymes, was knocked down by small interfering RNA transfection in differentiated KCs, only NQO-1 was down-regulated in both mRNA and protein levels. In human skin, expression levels of the phase 2 enzymes were up-regulated in the differentiated KC in the normal epidermis and keratotic foci in squamous cell carcinoma, further supporting the differentiation-dependent expression of phase 2 enzymes in vivo. This study demonstrates that a group of phase 2 enzymes are modulated during KC differentiation via either Nrf2-dependent (NQO-1) or Nrf2-independent (HO-1 and GSTpi) ways.

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Section: Discussionsupporting

confidence: 87%

Nrf2-dependent and Nrf2-independent induction of phase 2 detoxifying and antioxidant enzymes during keratinocyte differentiation

et al. 2012

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“…in the upper layers of epidermis) (22) and may suffer very low and high tempera- tures. Thus, at the human skin GSH concentration (about 1-3 mM) (23), GST P1-1 displays an almost unchanged affinity for GSH in a wild range of temperatures (e.g.…”

Section: Discussionmentioning

confidence: 99%

Temperature Adaptation of Glutathione S-Transferase P1–1

Caccuri

Antonini

Ascenzi

et al. 1999

Journal of Biological Chemistry

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Human glutathione S-transferase P1-1 (GST P1-1) is a homodimeric enzyme expressed in several organs as well as in the upper layers of epidermis, playing a role against carcinogenic and toxic compounds. A sophisticated mechanism of temperature adaptation has been developed by this enzyme. In fact, above 35°C, glutathione (GSH) binding to GST P1-1 displays positive cooperativity, whereas negative cooperativity occurs below 25°C. This binding mechanism minimizes changes of GSH affinity for GST P1-1 because of temperature fluctuation. This is a likely advantage for epithelial skin cells, which are naturally exposed to temperature variation and, incidentally, to carcinogenic compounds, always needing efficient detoxifying systems. As a whole, GST P1-1 represents the first enzyme which displays a temperature-dependent homotropic regulation of substrate (e.g. GSH) binding.

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“…In addition, positive nuclear staining may indicate that GST-P is accumulated in the nuclei of the pouch keratinocytes under the stimulus of DMBA during the process of chemical carcinogenesis. Nuclear staining of human placental GST isoenzyme has also been recognized in (pre)malignant lesions of human oral mueosa (10,11) and cutaneous tissues (34,35). Such nuclear staining has been regarded as critical in malignant transformation and linked to progression in transitional cell carcinoma ofthe bladder (36).…”

Section: Discussionmentioning

confidence: 99%

A study on betel quid chewing behavior among Kaohsiung residents aged 15 years and above

Chen

Shaw²

1996

J Oral Pathology Medicine

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A telephone survey was used to study betel quid chewing behavior of Kaohsiung residents aged 15 and above in early 1991. Among the 1,162 subjects, 154 (13.3%) reported that they chewed betel quids and most of them (145/154) were men. Among the chewers, 33 (21.4%) indicated that they chewed it daily; all were men. Age distribution of the chewing behavior suggested that more young people chewed it at the time of the survey than had been the case in the past. The demographic distributions of betel quid chewing behavior were similar to the characteristics of tobacco smoking in Taiwan. The degree of concurrence of these two behaviors was very high. Nearly 90% of the subjects believed that betel quid chewing would cause more harm than good, or it would cause only harm, to their health. Discriminant analyses indicated that smoking behavior and attitude toward the health effect of chewing betel quids were the two most significant and meaningful variables in prediction of the chewing behavior. Strategies for prevention are recommended.

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The expression of placental-type glutathione S-tranferase (GST-?) in human cutaneous squamous cell carcinoma and normal human skin

Cited by 17 publications

References 22 publications

Nrf2-dependent and Nrf2-independent induction of phase 2 detoxifying and antioxidant enzymes during keratinocyte differentiation

Nrf2-dependent and Nrf2-independent induction of phase 2 detoxifying and antioxidant enzymes during keratinocyte differentiation

Temperature Adaptation of Glutathione S-Transferase P1–1

A study on betel quid chewing behavior among Kaohsiung residents aged 15 years and above

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