The Expression of Toll-like receptors in eutopic and ectopic endometrium and its implication in the inflammatory pathogenesis of adenomyosis

Abstract

In this study, we investigated the expression profiles of Toll-like receptors(TLRs) in eutopic endometrium(EU) and ectopic endometrium(EC) and its implication in the inflammatory pathogenesis of adenomyosis. Thirty adenomyosis patients who underwent laparoscopy were recruited in this study. We tested the mRNA and protein expression of TLRs, and the mRNA expression of IL-6 and IL-8 in EU and EC of adenomyosis patients, and control endometrium without adenomyosis(CE). We found that the mRNA expression of IL-6 and IL-8 in EU was significantly higher than that in CE, and was the highest in EC (P < 0.01). The mRNA and protein expression of TLRs were higher in EU, with the expression of TLR1-6, 8 and 9 being significantly higher in EU than in CE, and were the highest in EC (except TLR6) (P < 0.05 or P < 0.01). Pearson correlation analysis showed that the expression of TLR1, 2, 4, 5 and 9 in EU and EC was positively correlated with that of IL-6 and IL-8 (P < 0.00139). This study suggested that adenomyosis was a state of inflammatory pathology. High expression of TLRs in EU and EC were positively correlated with IL-6 and IL-8, which may be involved in the inflammatory pathogenesis of adenomyosis.

Association between the HMGB1/TLR4 signaling pathway and the clinicopathological features of ovarian cancer

“…In addition, the correlation between the expression of HMGB1, TLR4, NF-κB and TNF-α in MEOC group was evaluated and it was demonstrated that all the parameters were positively correlated with each other. It is known that the HMGB1/TLR4 signaling pathway regulates cell proliferation and survival, and creates a tumor microenvironment that facilitates tumor growth by inducing immune cell expansion and integrating inflammatory responses (19). Considering these previous findings and those obtained from the present study, it was therefore concluded that increased HMGB1/TLR4 in the inflammatory immune signaling system may be involved in MEOC tumor stage and differentiation.…”

Bioinformatics strategy for the screening of key genes to differentiate adenomyosis from endometriosis (Review)

“…vii) TLR4. The expression of TLRs (TLR1, 2, 4, 5 and 9) is higher in the ectopic endometrium and eutopic endometrium in adenomyosis as compared with the normal endometrium, and positively correlates with IL-6 and IL-8 (74). Similar to adenomyosis, TLR4 is overexpressed in endometriosis, indicating that both conditions are a state of inflammatory pathology (75).…”

Proteomic Analysis of Uterine Tissues During Peri-Implantation Period in Mice with Experimentally Induced Adenomyosis that Treated with anti-Ngf: Implications for Cell-Cell Adhesion and Metabolic Processes

“…A noticeably reduced rate of spontaneous pregnancy was reported in women with concomitant adenomyosis who underwent surgical treatment of rectovaginal and bowel endometriosis compared with those without adenomyosis (11.9% vs. 43.0%) [ 3 ]. A number of scholars demonstrated that there were abnormal aggregation of inflammatory immune cells and cytokines in adenomyosis nodes, including dendritic cells, macrophages, neutrophils, tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), IL-8, nerve injury-induced protein1, and cyclooxygenase-2 (COX-2) [ 4 – 6 ]. In addition, disturbed uterine peristalsis, anatomical distortion of uterine cavity, altered uterine oxidative stress environment, and changing endometrial steroid metabolism may all contribute to the impaired endometrial receptivity in adenomyosis patients [ 7 – 9 ].…”