The expression pattern and cellular localisation of Myosin VI during the Drosophila melanogaster life cycle

Millo, Hadas; Bownes, Mary

doi:10.1016/j.modgep.2006.10.008

Cited by 4 publications

(4 citation statements)

References 15 publications

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“…The remaining two DRC-13 genes had no evidence for mesodermal expression ( fkh ) or no data available ( jar ). However, fkh was essential for caudal visceral mesoderm development [ 78 ] and jar was expressed in midgut mesoderm [ 79 ].…”

Section: Resultsmentioning

confidence: 99%

Functional Transcription Factor Target Networks Illuminate Control of Epithelial Remodelling

Overton

Sims

Owen

et al. 2020

Cancers

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Cell identity is governed by gene expression, regulated by transcription factor (TF) binding at cis-regulatory modules. Decoding the relationship between TF binding patterns and gene regulation is nontrivial, remaining a fundamental limitation in understanding cell decision-making. We developed the NetNC software to predict functionally active regulation of TF targets; demonstrated on nine datasets for the TFs Snail, Twist, and modENCODE Highly Occupied Target (HOT) regions. Snail and Twist are canonical drivers of epithelial to mesenchymal transition (EMT), a cell programme important in development, tumour progression and fibrosis. Predicted “neutral” (non-functional) TF binding always accounted for the majority (50% to 95%) of candidate target genes from statistically significant peaks and HOT regions had higher functional binding than most of the Snail and Twist datasets examined. Our results illuminated conserved gene networks that control epithelial plasticity in development and disease. We identified new gene functions and network modules including crosstalk with notch signalling and regulation of chromatin organisation, evidencing networks that reshape Waddington’s epigenetic landscape during epithelial remodelling. Expression of orthologous functional TF targets discriminated breast cancer molecular subtypes and predicted novel tumour biology, with implications for precision medicine. Predicted invasion roles were validated using a tractable cell model, supporting our approach.

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Section: Resultsmentioning

confidence: 99%

Functional Transcription Factor Target Networks Illuminate Control of Epithelial Remodelling

Overton

Sims

Owen

et al. 2020

Cancers

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“…Although Myosin VI is known to be important for Drosophila embryonic nervous system development [11,30,34], a function for Myosin VI at the NMJ has yet to be described and the locomotor defects observed in the jar maternal nulls mutant may reflect problems at this synapse. NMJ morphology was visualized in jar loss of function mutants by staining neuronal tissues with an FITC-conjugated anti-HRP antibody.…”

Section: Resultsmentioning

confidence: 99%

Myosin VI contributes to synaptic transmission and development at the Drosophila neuromuscular junction

et al. 2011

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BackgroundMyosin VI, encoded by jaguar (jar) in Drosophila melanogaster, is a unique member of the myosin superfamily of actin-based motor proteins. Myosin VI is the only myosin known to move towards the minus or pointed ends of actin filaments. Although Myosin VI has been implicated in numerous cellular processes as both an anchor and a transporter, little is known about the role of Myosin VI in the nervous system. We previously recovered jar in a screen for genes that modify neuromuscular junction (NMJ) development and here we report on the genetic analysis of Myosin VI in synaptic development and function using loss of function jar alleles.ResultsOur experiments on Drosophila third instar larvae revealed decreased locomotor activity, a decrease in NMJ length, a reduction in synaptic bouton number, and altered synaptic vesicle localization in jar mutants. Furthermore, our studies of synaptic transmission revealed alterations in both basal synaptic transmission and short-term plasticity at the jar mutant neuromuscular synapse.ConclusionsAltogether these findings indicate that Myosin VI is important for proper synaptic function and morphology. Myosin VI may be functioning as an anchor to tether vesicles to the bouton periphery and, thereby, participating in the regulation of synaptic vesicle mobilization during synaptic transmission.

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“…The remaining two DRC-13 genes had no evidence for mesodermal expression (fkh) or no data available (jar). However, fkh is essential for caudal visceral mesoderm development (Kusch and Reuter, 1999) and jar is expressed in midgut mesoderm (Millo and Bownes, 2007).…”

Section: Genome-scale Functional Transcription Factor Target Networkmentioning

confidence: 99%

Functionally Coherent Transcription Factor Target Networks Illuminate Control of Epithelial Remodelling

et al. 2018

Preprint

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Cell identity is governed by gene expression, regulated by Transcription Factor (TF) binding at cisregulatory modules. We developed the NetNC software to decode the relationship between TF binding and the regulation of cognate target genes in cell decision-making; demonstrated on nine datasets for the Snail and Twist TFs, and also modENCODE 'HOT' regions. Results illuminated conserved molecular networks controlling development and disease, with implications for precision medicine. Predicted 'neutral' TF binding accounted for the majority (50% to ≥80%) of candidate target genes from statistically significant peaks and HOT regions had high functional coherence.Expression of orthologous functional TF targets discriminated breast cancer molecular subtypes and predicted novel tumour biology. We identified new gene functions and network modules including crosstalk with notch signalling and regulation of chromatin organisation, evidencing networks that reshape Waddington's landscape during epithelial remodelling. Predicted invasion roles were validated using a tractable cell model, supporting our computational approach.

show abstract

The expression pattern and cellular localisation of Myosin VI during the Drosophila melanogaster life cycle

Cited by 4 publications

References 15 publications

Functional Transcription Factor Target Networks Illuminate Control of Epithelial Remodelling

Functional Transcription Factor Target Networks Illuminate Control of Epithelial Remodelling

Myosin VI contributes to synaptic transmission and development at the Drosophila neuromuscular junction

Functionally Coherent Transcription Factor Target Networks Illuminate Control of Epithelial Remodelling

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