The Expression Patterns of Guidance Receptors, DCC and Neogenin, Are Spatially and Temporally Distinct throughout Mouse Embryogenesis

Abstract

To gain a better understanding of the role of DCC and Neogenin in neural and nonneural tissues during vertebrate development we have carried out in situ hybridization studies to determine their expression patterns throughout the mid to late stages of mouse embryogenesis. This analysis revealed striking contrasts in both the spatial and temporal expression patterns of these closely related molecules. While DCC mRNA expression was predominantly restricted to the developing central nervous system (CNS), Neogenin mRNA was detected in a broad spectrum of embryonic tissues. Outside the CNS, Neogenin expression was observed mainly in mesodermal derivatives such as organ primordia and cartilage condensations of many developing embryonic structures. Within the CNS, initiation of DCC expression correlated with the onset of neurogenesis and was maintained at high levels in all regions of the developing CNS actively undergoing neurogenesis. By E18.5, DCC expression was detected only in structures such as the olfactory bulb, the hippocampus, and the cerebellum, that are known to sustain active neurogenesis well into postnatal life. In contrast, Neogenin expression was weak in the early developing CNS but broadened and intensified as neurogenesis proceeded. In summary, these observations indicate that Neogenin is the predominant member of this subfamily in mesodermal tissues, while DCC and Neogenin may play complementary roles in the generation of the fully functional CNS.

RGMa and RGMb expression pattern during chicken development suggest unexpected roles for these repulsive guidance molecules in notochord formation, somitogenesis, and myogenesis

“…The deeper muscles are incompletely stained, due to limited penetration of the tissue by probe and antibody. Expression domains of the Neogenin receptor according to (Fitzgerald et al, 2006;Gad et al, 1997;Mawdsley et al, 2004, this study); BMP2,4,6 (Andr ee et al Chapman et al, 2002;Danesh et al, 2009;Zhou et al, 2010), Shh (Roelink et al, 1995;Marti et al, 1995), mouse RGMa and RGMb (Schmidtmer and Engelkamp, 2004;Niederkofler et al, 2004;Oldekamp et al, 2004); Xenopus RGMa and RGMb Wilson and Key, 2006;Gessert et al, 2008;Kee et al, 2008), and zebrafish RGMa and RGMb (Samad et al, 2004;Bian et al, 2011). Hyphens indicate absence of corresponding expression domains for the chicken paralogs.…”

Cocaine-Induced Intracellular Signaling and Gene Expression Are Oppositely Regulated by the Dopamine D1 and D3 Receptors

“…Neogenin belongs to a distinct subfamily of NCAM-like cell surface receptors that is expressed during terminal neuronal differentiation and is also involved in neurite outgrowth (Vielmetter et al, 1994;Gad et al, 1997). Neogenin, together with its ligands, the netrins, is a major guidance system in neural cell migration (KeinoMasu et al, 1996;Keynes and Cook, 1996;Tessier-Lavigne and Goodman, 1996).…”

Netrin-1 expression in fetal and regenerating rat pancreas and its effect on the migration of human pancreatic duct and porcine islet precursor cells

“…However, direct evidence for this is lacking at the moment. Studies on the expression of neogenin and DCC done by in situ hybridisation in the mouse embryo already indicated that neogenin is the predominant member of this subfamily in other tissues than the neuronal tissue, while DCC and neogenin could play complementary roles in the generation of the ful- ly functional central nervous system [28]. However, other families of netrin receptors will have to be considered, such as the vertebrate unc-5 homologues [29] which were shown to convert the netrin-induced chemoattraction via DCC into repulsion when both receptors are simultaneously expressed [30,31].…”