The Expressions of Cancer Stem Cell Markers and Nonclassical HLA antigens in Breast Tumors

Özdemir, Rabia Bilge Özgül; Özdemir, Alper Tunga; Oltulu, Fatih; Kurt, Kamile; Yiğittürk, Gürkan; Kırmaz, Cengiz

doi:10.5152/imj.2017.63497

Cited by 2 publications

(1 citation statement)

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“…Most have been performed using immunohistochemical methods and have reported a strong correlation between increased HLA-G expression and poor tumor prognosis [15,23]. In BC, HLA-G is also associated with poor prognosis, and some researchers determined that HLA-G might be increased in a subset of tumor cells called cancer stem cells [24,25]. Based on the literature data, we investigated the HLA-G expression status of IFN-gstimulated invasive breast tumor cells; i.e., MDA-MB-231 and non-invasive MCF-7 cells and explored how MSCs affected this status.…”

Section: Discussionmentioning

confidence: 99%

The effects of mesenchymal stem cells on the IDO, HLA-G and PD-L1 expression of breast tumor cells MDA-MB-231 and MCF-7

Özdemir¹,

Özdemir

Kırmaz

et al. 2019

Archives of Clinical and Experimental Medicine

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Aim: Mesenchymal stem cells (MSCs) are strong immunomodulatory cells and a component of the tumor microenvironment. In this study, we aimed to investigate the effects of MSCs derived from adipose tissue on the expressions of immune evasive molecules indoleamine 2,3-dioxygenase (IDO), human leukocyte antigen G (HLA-G) and programmed death-ligand 1 (PD-L1) of breast tumor cell lines MDA-MB-231 and MCF-7. Methods: For this purpose, MSCs, MDA-MB-231 and MCF-7 cells were cultured with increased doses of interferon gamma (IFN-g). In another plate, tumor cells were cultured in transwell inserts using the same IFN-g stimulation to evaluate the effect of MSCs. At the end of the culture period, the HLA-G and PD-L1 expression was detected by flow cytometry, and IDO expression by the Luminex method. Results: We found that in low-dose IFN-g stimulation (10 ng/mL), MSCs led to a significant increase in the HLA-G and PD-L1 expression of MCF-7 cells. On the contrary, at a high dose of IFN-g (50 ng/mL), their expression significantly decreased in both tumor cells. In addition, we observed that the IDO expression of MDA-MB-231 cells was significantly increased in the presence of MSCs, but MCF-7 cells were not affected. Conclusion: In conclusion, for MDA-MB-231 cells, MSCs may play a protective role because they reduce the expression of HLA-G and PD-L1 that are involved in the suppression of cytotoxic cells and exhaustion of T cells. On the other hand, MSCs may be an important source of high IDO levels, and therefore may negatively affect the antitumor immune response. However, our data should be supported by further studies.

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