The extent of base pairing in 5 s RNA Yeast 5 s RNA

Wong, Yeng P.; Kearns, David R.; Reid, Brian R.; Shulman, Robbet G.

doi:10.1016/0022-2836(72)90188-x

Cited by 30 publications

(15 citation statements)

References 19 publications

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“…By Raman spectroscopy (2,3) a similar high degree of base pairing has been postulated. Both oligonucleotide binding studies (25) and NMR-spectroscopy (5,27) yield a smaller degree of base pairing. By both methods a lower 1)Including the numbers of GU base pairs.…”

Section: Discussionmentioning

confidence: 99%

Determination of base pairing in yeast 5S and 5.8S RNA by infrared spectroscopy

Stulz¹,

Ackermann²,

Appel³

et al. 1981

Nucl Acids Res

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Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are large contributions of tertiary interactions. The results are compared with data derived from structural models that were proposed in the literature as well as with data previously published for prokaryotic 5S RNAs.

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Section: Discussionmentioning

confidence: 99%

Determination of base pairing in yeast 5S and 5.8S RNA by infrared spectroscopy

Stulz¹,

Ackermann²,

Appel³

et al. 1981

Nucl Acids Res

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“…The majority of NMR studies of intact 5S RNA have focused on the imino proton spectral region in H 2 O solution with somewhat better spectral resolution observed on proceeding from low-field (Wong et al 1972;Salemink et al 1981) to high-field the imino proton spectra (10-15 ppm) of (a) intact E. colt 5S RNA and (6) its RNase A-resistant fragment in oi M-KC1, 5 mM cacodylate, 4 mM Mg, pH 7-2 and 30 °C (Kime & Moore, 1983). (c) A resolution enhanced spectrum of the ribonuclease-resistant fragment of E. colt 5S RNA with the convention for naming resonances given above the spectrum (Leontis et al 1986).…”

Section: Intact 5s Rnamentioning

confidence: 99%

DNA and RNA: NMR studies of conformations and dynamics in solution

Patel

Shapiro

Hare

1987

Quart. Rev. Biophys.

183

167

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The early NMR research on nucleic acids was of a qualitative nature and was restricted to partial characterization of short oligonucleotides in aqueous solution. Major advances in magnet design, spectrometer electronics, pulse techniques, data analysis and computational capabilities coupled with the availability of pure and abundant supply of long oligonucleotides have extended these studies towards the determination of the 3-D structure of nucleic acids in solution.

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“…Optical (14)(15)(16), Raman (17) and chemical methods (9,18) have given equilibrium properties of eukaryotic 5S RNA, in which a high degree of base paring and a 0 IRL Press Umited, Oxford, England. largely double helical configuration have also been expected to exist.…”

Section: Introductionmentioning

confidence: 99%

Melting of local ordered structures in yeast 5s ribosomsal RNA in aqueous salts

Ohta¹,

Maruyama²,

Nitta³

et al. 1983

Nucl Acids Res

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Equilibrium and kinetics of thermal melting of yeast 5S ribosomal RNA in aqueous NaCl with or without Mg2+ were investigated by differential thermal melting and temperature jump methods. Two peaks (1 and 2) and a shoulder were observed in each of melting curves at ionic strength I=0.002-0.5 and linearity between each of melting temperatures T1m and T2m and log I was found at I=0.01-0.5 in the Mg2+-free solution. The local structures were found to be stabilized considerably by Mg2+. The temperature jump measurements gave the kinetic melting curve of the structure 1 at I=0.03 without Mg2+ or with 0.5 mM Mg2+. The kinetic Tm coincided well with the corresponding static Tm. For the structure 1, various parameters were calculated from the kinetic data, which indicated a double helical character of the structure 1. In terms of the values of Tm, G-C content, and enthalpy change of the transition of the structure 1 or 2, appropriateness of each of the secondary structure models of eukaryotic 5S RNA proposed previously was discussed.

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The extent of base pairing in 5 s RNA Yeast 5 s RNA

Cited by 30 publications

References 19 publications

Determination of base pairing in yeast 5S and 5.8S RNA by infrared spectroscopy

Determination of base pairing in yeast 5S and 5.8S RNA by infrared spectroscopy

DNA and RNA: NMR studies of conformations and dynamics in solution

Melting of local ordered structures in yeast 5s ribosomsal RNA in aqueous salts

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