The external NADH dehydrogenases of intact plant mitochondria

Douce, Roland; Mannella, Carmen A.; Bonner, Walter D.

doi:10.1016/0005-2728(73)90255-7

Cited by 224 publications

(120 citation statements)

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“…Fredlund and I.M. Moller, unpublished results) consistent with the observation by Deuce et al [19] whereas all the NAD(P)H dehydrogenases in the inner membrane are /I-specific ( [21]; K.M. Fredlund and 1-M. Msller, unpublished results).…”

Section: Resultssupporting

confidence: 86%

“…When mung bean [19] or potato [ZO] mitoc~on~ia are suspended in a hypo-osmolar medium (20-40 mosM final concentration) they swell, the outer membrane ruptures and some of it is released into the medium. When these outer membranes are isolated as described in section 2, they contain minimal contamination by matrix, as shown by the absence of detectable MDH activity, and minimal contamination by inner membrane, as shown by low specific activity of CC0 (Table I).…”

Section: Resultsmentioning

confidence: 99%

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The outer membrane of plant mitochondria contains a calcium‐dependent protein kinase and multiple phosphoproteins

et al. 1993

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Highly purified mitochondria from potato (S&men tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [$'P]ATP only one and three prominent phosphoproteins were detected after SDS-PAGE and autoradiography, respectively. In contrast, more than 20 phosphoproteins could be labelled in the outer membrane fraction, the main ones at 12, l&26,43, 58, 60, 65, 74 and 110 kDa. Only one band, at 18 kDa, was detectable when the labelling was done in the presence of EGTA. We conclude that the outer membrane of plant mitochondria contains at least one Ca*'-dependent protein kinase and more than 20 endogenous substrates.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

The outer membrane of plant mitochondria contains a calcium‐dependent protein kinase and multiple phosphoproteins

et al. 1993

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“…Plant mitochondria have an external rotenone-insensitive NADH dehydrogenase on the outer face of the inner mitochondrial membrane as well as a matrix NADH dehydrogenase linked to the rotenone sensitive oxidation of tricarboxylic acid cycle substrates (7). Is the NAD-responsive, membrane-associated ICDH described herein linked to the external inner membrane NADH dehydrogenase?…”

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confidence: 98%

Membrane-Associated NAD-Dependent Isocitrate Dehydrogenase in Potato Mitochondria

Laties

1983

Plant Physiol.

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The oxidation isotherms for citrate and isocitrate by potato (Solanm tuberosum var. Russet Burbank) mitochondria in the presence of NAD differ markedly. Citrate oxidation shows positively cooperative kinetics with a sigmoid isotherm, whereas isocitrate oxidation shows MichaelisMenten kinetics at concentrations up to 3 millimolar, and cooperative kinetics thereafter up to 30 millimolar. In the absence of exogenous NAD, the isocitrate isotherm is sigmoid throughout. The dual isotherm for isocitrate oxidation in the presence of exogenous NAD reflects the operation of two forms of isocitrate dehydrogenase, one in the matrix and one associated with the inner mitochondrial membrane. Whereas in intact mitochondria the activity of the membrane-bound enzyme is insensitive to rotenone, and to butylmalonate, an inhibitor of organic acid transport, isocitrate oxidation by the soluble matrix enzyme is inhibited by both. The membrane-bound isocitrate dehydrogenase does not operate through the NADH dehydrogenase on the outer face of the inner mitochondrial membrane, and is thus considered to face inward. The regulatory potential of isocitrate dehydrogenase in potato mitochondria may be realized by the apportionment of the enzyme between its soluble and bound forms.In an early study, fresh potato slices were found to lack full' tricarboxylic acid cycle activity in consequence of their inability to oxidize citrate to a-ketoglutarate (15). At the same time NADlinked isocitrate dehydrogenase was shown to exhibit positively cooperative kinetics (12,22), and accordingly, ICDH2 was proposed as a potential regulator ofthe tricarboxylic acid cycle subject to modulation and control by an array of cofactors and metabolic intermediates (1,6,8,9,12). Subsequently, Ribereau-Gayon and Laties (20,21) demonstrated the sigmoidicity of the isotherm for isocitrate oxidation in potato mitochondria together with the socalled activating effect of citrate, which both lowered the SO.5 for isocitrate and seemingly converted the isotherm from sigmoid to hyperbolic (see below). During this period, the details of di-and tricarboxylic acid transport across the inner mitochondrial membrane received exhaustive attention, and it was established that carrier-mediated tricarboxylic acid uptake into the mitochondrion was dependent upon concomitant outward transport of dicarboxylic acid-malate in particular-the latter, in turn, being taken up from the milieu in exchange for intramitochondrial phosphate (3

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“…Recent evidence [5,6] suggesting that extramitochondrial NADH oxidation occurs via a dehydrogenase located on the outer surface of the inner membrane necessitates a re-examination of the monovalent cation stimulation.…”

Section: Introductionmentioning

confidence: 99%

The mechanism of K⁺‐stimulated exogenous NADH oxidation in plant mitochondria

Earnshaw

1975

FEBS Letters

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The external NADH dehydrogenases of intact plant mitochondria

Cited by 224 publications

References 19 publications

The outer membrane of plant mitochondria contains a calcium‐dependent protein kinase and multiple phosphoproteins

The outer membrane of plant mitochondria contains a calcium‐dependent protein kinase and multiple phosphoproteins

Membrane-Associated NAD-Dependent Isocitrate Dehydrogenase in Potato Mitochondria

The mechanism of K⁺‐stimulated exogenous NADH oxidation in plant mitochondria

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The external NADH dehydrogenases of intact plant mitochondria

Cited by 224 publications

References 19 publications

The outer membrane of plant mitochondria contains a calcium‐dependent protein kinase and multiple phosphoproteins

The outer membrane of plant mitochondria contains a calcium‐dependent protein kinase and multiple phosphoproteins

Membrane-Associated NAD-Dependent Isocitrate Dehydrogenase in Potato Mitochondria

The mechanism of K+‐stimulated exogenous NADH oxidation in plant mitochondria

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The mechanism of K⁺‐stimulated exogenous NADH oxidation in plant mitochondria