The Extracts of Some Marine Invertebrates and Algae Collected off the Coast Waters of Vietnam Induce the Inhibitory Effects on the Activator Protein‐1 Transcriptional Activity in JB6 Cl41 Cells

Abstract

It has previously been shown that inhibition of the transcriptional activity of the oncogenic nuclear factor AP-1 can result in cancer prevention. Marine invertebrates and alga are a rich source of natural compounds that possess various biological activities. The inhibitory effects of the extracts of Vietnamese marine organisms in relation to the AP-1 transcriptional activity were examined by the luciferase method using JB6 Cl41 cells stably expressing a luciferase reporter gene controlled by AP-1 DNA binding sequence. As was found, 71 species of marine sponges out of 148 species studied contain inhibitors of the AP-1 transcriptional activity. Therefore, marine organisms as a source of biologically active compounds have a great potential for isolation of the new cancer preventive compounds that inhibit the oncogenic AP-1 nuclear factor.

Aaptamines from the Marine SpongeAaptossp. Display Anticancer Activities in Human Cancer Cell Lines and Modulate AP-1-, NF-κB-, and p53-Dependent Transcriptional Activity in Mouse JB6 Cl41 Cells

“…The effects of the substances on the basal transcriptional activities of AP-1, NF- κ B, or p53 nuclear factor were evaluated using JB6 Cl41 cell lines stably expressing a luciferase reporter gene controlled by an AP-1-, NF- κ B-, or p53-DNA binding sequence, as described previously [ 14 ]. Briefly, cells were preincubated overnight in 96-well plates (20 × 10 3 cells/well) in 100 μ L/well of culture medium.…”

Achievements in the Study of Marine Low‐Molecular Weight Biologically Active Metabolites from the Vietnamese Territorial Waters as a Result of Expeditions aboard the Research Vessel ‘Akademik Oparin’ (2004–2017)

“…[23,24] Moreover, the effects of extracts obtained in 2007 on the AP-1 oncogenic nuclear factor was investigated by the luciferase method using JB6 Cl 41 cells stably expressing a luciferase reporter gene controlled by the AP-1 DNAbinding sequence. [25] The extracts of 71 species of marine organisms showed inhibitory effects on the AP-1 transcriptional activity in JB6 Cl 41 AP-1 cells, and only 12 extracts demonstrated activation of the AP-1 nuclear factor. Inhibition of the AP-1 transcriptional activity was shown for extracts of Spongia, Petrosia, Halichondria, Callyspongia, Spirastrella, and Axinella genera of sponges, and activation of the AP-1 factor was shown for Opisthobranchia and Algae extracts.…”

Marine Low Molecular Weight Natural Products as Potential Cancer Preventive Compounds

“…Recent progress in the research of marine anticancer compounds resulted in identification of various molecular targets for chemopreventive drugs, such as indoleamine 2,3-dioxygenase, histone deacetylases and methyl-transferases, matrix metalloproteinases (MMPs), hypoxia-inducible factor-1 (HIF-1), retinoic acid receptor, peroxisome proliferator-activated receptor (PPAR) isoforms, ubiquitin-proteasome pathway, oncogenic nuclear factors activator protein-1 (AP-1) and nuclear factor-κB (NF-κB), tumor suppressor protein p53-mouse double minute 2 protein (Mdm2) interaction, different tumor-related kinases, etc. [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ].…”