The F-actin filament capping protein CapG is a bona fide nucleolar protein

Hubert, Thomas; Impe, Katrien Van; Vandekerckhove, Joël; Gettemans, Jan

doi:10.1016/j.bbrc.2008.10.048

Cited by 15 publications

(10 citation statements)

References 27 publications

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“…By subcellular fractioning this matches with our findings (unpublished data) that SK-OV-3 cells, which display a highly invasive phenotype, contain a considerable amount of nuclear CapG protein. This becomes even more evident since nuclear complexes have been identified to carry nuclear actin and can be modified by actin-binding proteins such as CapG [25]. One of them is the BAF (Brg1/Brm-associated factor) complex which modulates DNA transcription in the nucleus [26, 27].…”

Section: Discussionmentioning

confidence: 99%

Macrophage Capping Protein CapG Is a Putative Oncogene Involved in Migration and Invasiveness in Ovarian Carcinoma

Glaser

Neumann

Mei

et al. 2014

BioMed Research International

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The actin binding protein CapG modulates cell motility by interacting with the cytoskeleton. CapG is associated with tumor progression in different nongynecologic tumor entities and overexpression in breast cancer cell lines correlates with a more invasive phenotype in vitro. Here, we report a significant CapG overexpression in 18/47 (38%) of ovarian carcinomas (OC) analyzed by qRealTime-PCR analyses. Functional analyses in OC cell lines through siRNA mediated CapG knockdown and CapG overexpression showed CapG-dependent cell migration and invasiveness. A single nucleotide polymorphism rs6886 inside the CapG gene was identified, affecting a CapG phosphorylation site and thus potentially modifying CapG function. The minor allele frequency (MAF) of SNP rs6886 (c.1004A/G) was higher and the homozygous (A/A, His335) genotype was significantly more prevalent in patients with fallopian tube carcinomas (50%) as in controls (10%). With OC being one of the most lethal cancer diseases, the detection of novel biomarkers such as CapG could reveal new diagnostic and therapeutic targets. Moreover, in-depth analyses of SNP rs6886 related to FTC and OC will contribute to a better understanding of carcinogenesis and progression of OC.

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Section: Discussionmentioning

confidence: 99%

Macrophage Capping Protein CapG Is a Putative Oncogene Involved in Migration and Invasiveness in Ovarian Carcinoma

Glaser

Neumann

Mei

et al. 2014

BioMed Research International

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“…CapG expression is high in tissues in which macrophages are found (Table ). CapG is present in both the nucleus and the cytoplasm [Onoda et al, ; Hubert et al, ; Hubert et al, ] and has roles in cell differentiation, membrane ruffling, phagocytosis and cell motility [Onoda et al, ; Parikh et al, ; Sun at al., ]. CapG has recently also been shown to be involved in cell invasion and metastasis, being highly overexpressed in several metastatic cancers.…”

Section: The Gelsolin Superfamilymentioning

confidence: 99%

Gelsolin: The tail of a molecular gymnast

et al. 2013

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Gelsolin superfamily members are Ca 21 -dependent, multidomain regulators of the actin cytoskeleton. Calcium binding activates gelsolin by inducing molecular gymnastics (large-scale conformational changes) that expose actin interaction surfaces by releasing a series of latches. A specialized tail latch has distinguished gelsolin within the superfamily. Active gelsolin exhibits actin filament severing and capping, and actin monomer sequestering activities. Here, we analyze a combination of sequence, structural, biophysical and biochemical data to assess whether the molecular plasticity, regulation and actinrelated properties of gelsolin are also present in other superfamily members. We conclude that all members of the superfamily will be able to transition between a compact conformation and a more open form, and that most of these open forms will interact with actin. Supervillin, which lacks the severing domain 1 and the F-actin binding-site on domain 2, is the clear exception. Eight calcium-binding sites are absolutely conserved in gelsolin, adseverin, advillin and villin, and compromised to increasing degrees in CapG, villin-like protein, supervillin and flightless I. Advillin, villin and supervillin each contain a potential tail latch, which is absent from CapG, adseverin and flightless I, and ambiguous in villin-like protein. Thus, calcium regulation will vary across the superfamily. Potential novel isoforms of the superfamily suggest complex regulation at the gene, transcript and protein levels. We review animal, clinical and cellular data that illuminate how the regulation of molecular flexibility in gelsolin-like proteins permits cells to exploit the force generated from actin polymerization to drive processes such as cell movement in health and disease. V C 2013 Wiley Periodicals, Inc.

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“…In the cytoplasm, to control actin filament growth the barbed ends are capped by gelsolin-like proteins, which are also suggested to work as transcriptional co-activators 88 . CapG, one of the family members, represents an example of gelsolin-like proteins being actively imported in the cell nucleus and to localize also in the nucleolus 89 , 90 . Furthermore a large fraction of CapG freely diffuses but there is a smaller fraction of about 12% that appears to be slowed down in diffusion by 100-fold.…”

Section: Future Directionsmentioning

confidence: 99%

Co-transcriptional nuclear actin dynamics

Percipalle

2013

Nucleus

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Actin is a key player for nuclear structure and function regulating both chromosome organization and gene activity. In the cell nucleus actin interacts with many different proteins. Among these proteins several studies have identified classical nuclear factors involved in chromatin structure and function, transcription and RNA processing as well as proteins that are normally involved in controlling the actin cytoskeleton. These discoveries have raised the possibility that nuclear actin performs its multi task activities through tight interactions with different sets of proteins. This high degree of promiscuity in the spectrum of protein-to-protein interactions correlates well with the conformational plasticity of actin and the ability to undergo regulated changes in its polymerization states. Several of the factors involved in controlling head-to-tail actin polymerization have been shown to be in the nucleus where they seem to regulate gene activity. By focusing on the multiple tasks performed by actin and actin-binding proteins, possible models of how actin dynamics controls the different phases of the RNA polymerase II transcription cycle are being identified.

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The F-actin filament capping protein CapG is a bona fide nucleolar protein

Cited by 15 publications

References 27 publications

Macrophage Capping Protein CapG Is a Putative Oncogene Involved in Migration and Invasiveness in Ovarian Carcinoma

Macrophage Capping Protein CapG Is a Putative Oncogene Involved in Migration and Invasiveness in Ovarian Carcinoma

Gelsolin: The tail of a molecular gymnast

Co-transcriptional nuclear actin dynamics

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