The Family Carnobacteriaceae

Lawson, Paul A.; Caldwell, Matthew E.

doi:10.1007/978-3-642-30120-9_209

Cited by 9 publications

(6 citation statements)

References 238 publications

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“…Our findings suggest that the differential abundances of Carnobacteriaceae most strongly contribute to seasonal loadings in the River Cam. Carnobacteriaceae have been previously associated with a range of low-temperature environments ( Lawson and Caldwell, 2014 ), and we found these taxa to be more abundant in colder April samples (mean 11.3°C, vs. 15.8°C in June and 19.1°C in August). This might help to further establish this family as an indicator for bacterial community shifts along with temperature fluctuations, albeit the influence of co-occurring hydrochemical trends such as water hardness, dissolved carbon or flow speed changes should also be noted ( Figure 6b–d ; Figure 6—figure supplement 1d ).…”

Section: Discussionsupporting

confidence: 57%

“…The strongest differential abundances along the seasonal axis of variation (PC3) derived from Carnobacteriaceae ( Figure 5b ), a trend also highlighted by taxon-specific log-normal mixture model decomposition between the two seasons (April vs. June/August; p < 0.01; Materials and methods). Indeed, members of this bacterial family have been primarily isolated from cold substrates ( Lawson and Caldwell, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

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Freshwater monitoring by nanopore sequencing

Urban

Holzer

Baronas

et al. 2021

eLife

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While traditional microbiological freshwater tests focus on the detection of specific bacterial indicator species, including pathogens, direct tracing of all aquatic DNA through metagenomics poses a profound alternative. Yet, in situ metagenomic water surveys face substantial challenges in cost and logistics. Here, we present a simple, fast, cost-effective and remotely accessible freshwater diagnostics workflow centred around the portable nanopore sequencing technology. Using defined compositions and spatiotemporal microbiota from surface water of an example river in Cambridge (UK), we provide optimised experimental and bioinformatics guidelines, including a benchmark with twelve taxonomic classification tools for nanopore sequences. We find that nanopore metagenomics can depict the hydrological core microbiome and fine temporal gradients in line with complementary physicochemical measurements. In a public health context, these data feature relevant sewage signals and pathogen maps at species level resolution. We anticipate that this framework will gather momentum for new environmental monitoring initiatives using portable devices.

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Section: Discussionsupporting

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Freshwater monitoring by nanopore sequencing

Urban

Holzer

Baronas

et al. 2021

eLife

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“…has also been seen in other studies of sand filtration (8,26). Bacteria from groups commonly associated with fecal matter that appear to be preferentially removed (higher relative abundance in the influent than the effluent) include Epsilonproteobacteria (the most abundant genus was Arcobacter) (42,56), Fusobacteriia (57,58), Bacilli (most abundant groups include Streptococcaceae and Carnobacteriaceae) (59,60), and Verruco-5 (61). The class Coriobacteriia, which contains facultative anaerobes (62), was also more abundant in the influent than the effluent.…”

Section: Figsupporting

confidence: 63%

Microbial Dynamics of Biosand Filters and Contributions of the Microbial Food Web to Effective Treatment of Wastewater-Impacted Water Sources

Webster

Fierer

2019

Appl Environ Microbiol

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Biosand filtration systems are widely used for drinking water treatment, from household-level, intermittently operated filters to large-scale continuous municipal systems. While it is well-established that microbial activity within the filter is essential for the removal of potential pathogens and other contaminants, the microbial ecology of these systems and how microbial succession relates to their performance remain poorly resolved. We determined how different source waters influence the composition, temporal dynamics, and performance of microbial communities in intermittently operated biosand filters. We operated lab-scale biosand filters, adding daily inputs from two contrasting water sources with differing nutrient concentrations and found that total coliform removal increased and became less variable after 4 weeks, regardless of water source. Total effluent biomass was also lower than total influent biomass for both water sources. Bacterial community composition, assessed via cultivation-independent DNA sequencing, varied by water source, sample type (influent, effluent, or sand), and time. Despite these differences, we identified specific taxa that were consistently removed, including common aquatic and wastewater bacteria. In contrast, taxa consistently more abundant in the sand and effluent included predatory, intracellular, and symbiotic bacteria. IMPORTANCE Although microbial activities are known to contribute to the effectiveness of biosand filtration for drinking water treatment, we have a limited understanding of what microbial groups are most effectively removed, colonize the sand, or make it through the filter. This study tracked the microbial communities in the influent, sand, and effluent of lab-scale, intermittently operated biosand filters over 8 weeks. These results represent the most detailed and time-resolved investigation of the microbial communities in biosand filters typical of those implemented at the household level in many developing countries. We show the importance of the microbial food web in biosand filtration, and we identified taxa that are preferentially removed from wastewater-impacted water sources. We found consistent patterns in filter effectiveness from source waters with differing nutrient loads and, likewise, identified specific bacterial taxa that were consistently more abundant in effluent waters, taxa that are important targets for further study and posttreatment.

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“…However, one of our most striking findings was the identification of potential beneficial taxa of the order Lactobacillales as being more prevalent and abundant in the healthy subjects compared to the CRS patients. This order of lactic acid bacteria (LAB) encompasses taxa from the family of the Lactobacillaceae , including well-characterized lactobacilli ( De Boeck et al., 2020 ), and from the family of Carnobacteriaceae ( Lawson and Caldwell, 2014 ), especially the species Dolosigranulum pigrum ( De Boeck et al., 2019 ). Compared to (nasal) lactobacilli that could be cultured and studied in lab, animal and even in human trials ( De Boeck et al., 2020 ; Martens et al., 2021 ; De Rudder et al., 2020 ), nasal Carnobacteriaceae turned out to be more difficult to culture and study at functional level.…”

Section: Introductionmentioning

confidence: 99%

The nasal mutualist Dolosigranulum pigrum AMBR11 supports homeostasis via multiple mechanisms

et al. 2021

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Summary Comparing the nasal microbiome of healthy individuals and chronic rhinosinusitis (CRS) patients revealed Dolosigranulum pigrum as a species clearly associated with nasal health, although isolates obtained from healthy individuals are scarce. In this study, we explored the properties of this understudied lactic acid bacterium by integrating comparative genomics, habitat mining, cultivation, and functional characterization of interaction capacities. Mining 10.000 samples from the Earth Microbiome Project of 17 habitat types revealed that Dolosigranulum is mainly associated with the human nasal cavity. D. pigrum AMBR11 isolated from the nose of a healthy individual exerted antimicrobial activity against Staphylococcus aureus , decreased proinflammatory cytokine production in airway epithelial cells, and Galleria mellonella larvae mortality induced by this important nasal pathobiont. Furthermore, the strain protected the nasal barrier function in a mouse model using interleukin-4 as disruptive cytokine. Hence, D. pigrum AMBR11 is a mutualist with high potential as topical live biotherapeutic product.

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The Family Carnobacteriaceae

Cited by 9 publications

References 238 publications

Freshwater monitoring by nanopore sequencing

Freshwater monitoring by nanopore sequencing

Microbial Dynamics of Biosand Filters and Contributions of the Microbial Food Web to Effective Treatment of Wastewater-Impacted Water Sources

The nasal mutualist Dolosigranulum pigrum AMBR11 supports homeostasis via multiple mechanisms

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