The family Coriobacteriaceae: reclassification of Eubacterium exiguum (Poco etal. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov.

Wade, William G.; Downes, Julia; Dymock, David; Hiom, Sarah; Weightman, Andrew J.; Dewhirst, Floyd E.; Paster, Bruce J.; Tzellas, Nia; Coleman, Brittney

doi:10.1099/00207713-49-2-595

Cited by 167 publications

(120 citation statements)

References 25 publications

(28 reference statements)

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“…Sequences encoding the 16S rRNA of organisms related to strain HE8 were obtained using the BLAST service of the NCBI website (http://blast.ncbi.nlm .nih.gov/Blast.cgi). Sequence alignment using the Bioedit software (Ibis Biosciences, Carlsbad, CA) revealed 91% identity of the 16S rRNA gene sequence (1,339 bp) of strain HE8 with the closest described relatives, Slackia exigua isolated from human oral lesions (91.4%), Slackia faecicanis from dog feces (90.9%), and Slackia heliotrinireducens from sheep rumen (90.6%) (15,35). Lower values of sequence identity were observed for strain HE8 and the equol-forming species Adlercreutzia equolifaciens (90.2%), Eggerthella sp.…”

Section: Vol 75 2009 Daidzein and Genistein Conversion 1741mentioning

confidence: 99%

Isolation of a Human Intestinal Bacterium Capable of Daidzein and Genistein Conversion

Matthies

Blaut

Braune

2009

Appl Environ Microbiol

147

123

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Section: Vol 75 2009 Daidzein and Genistein Conversion 1741mentioning

confidence: 99%

Isolation of a Human Intestinal Bacterium Capable of Daidzein and Genistein Conversion

Matthies

Blaut

Braune

2009

Appl Environ Microbiol

147

123

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“…Previously, we have used the PCR procedure (4, 5) for identifying Collinsella (former genus name, Eubacterium) aerofadens and Eggerthella (former genus name, Eubacterium) lenta (13).…”

mentioning

confidence: 99%

Rapid Detection of Human Fecal Eubacterium Species and Related Genera by Nested PCR Method

Kageyama

Benno

2001

Microbiology and Immunology

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PCR procedures based on 16S rDNA gene sequence specific for seven Eubacterium spp. and Eggerthella lenta that predominate in the human intestinal tract were developed, and used for direct detection of these species in seven human feces samples. Three species of Eggerthella lenta, Eubacterium rectale, and Eubacterium eligens were detected from seven fecal samples. Eubacterium biforme was detected from six samples. It was reported that E. rectale, E. eligens, and E. biforme were difficult to detect by traditional culture method, but the nested PCR method is available for the detection of these species. This result shows that the nested PCR method utilizing a universal primer pair, followed by amplification with species-specific primers, would allow rapid detection of Eubacterium species in human feces.Key words: Species-specific primers, Nested PCR, Fecal floraIntestinal microflora in humans and animals is extremely complex and consists of several microorganisms, including anaerobes and aerobes (9). Although the genus Eubacterium isolated from human feces comprises several species of the predominant microorganisms (I), the isolation and identification of these species is very difficult (3). The traditional methods for identifying fecal bacteria include various culture techniques, bacteriological isolations, morphological examination, and biochemical tests. These methods are extremely laborintensive and time-consuming. In addition, they sometimes cannot distinguish the bacteria at the species level. In order to study such a complex species effectively, it is necessary to have quick and accurate method for identification of these species.In contrast, a rapid identification method using a PCR-based system (12) is very available. Previously, we have used the PCR procedure (4, 5) for identifying Collinsella (former genus name, Eubacterium) aerofadens and Eggerthella (former genus name, Eubacterium) lenta (13).In this study, we designed specific primers for another seven Eubacterium species; Eubacterium limosum, (8), and checked their specificity for each taxonomically related microorganism. Furthermore, we tried to identify each of these predominant Eubacterium species from direct fecal samples. The species-specific primers used in this study are listed in Table I. These primers were designed by comparing each 16S rRNA gene sequence with related species from GenBank or our own sequence data. We designed the species-specific primers so that the G +C content was about 50% and the number of bases about 20. Suspending the cultured colony in distilled water, heating at 100 C for 5 min and then cooling prepared bacterial DNA. Fresh feces were collected from healthy humans and analyzed by PCR. The fecal sample preparation method was that referred to in Wang et aI (14). The annealing temperatures used were 60 C or 63 C and cycle numbers were 25. The amplification was performed using a positive control and negative control by the PCR method. After PCR, an 8~I aliquot of amplified sample from each PCR tube was electro...

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“…The novel strains HM-6, HM-7 T and HH-31 were isolated from human tongue plaque (Hori et al, 1999), and strains P9a-h T and UJB13-d were also isolated from necrotic dental pulp (Sundqvist, 1976 ;Sato et al, 1993b). The type strains of previously established AAGPR species, together with the more recently proposed species, Eubacterium minutum (Poco et al, 1996b), Slackia exigua which was emended from Eubacterium exiguum (Poco et al, 1996a ;Wade et al, 1999), Cryptobacterium curtum (Nakazawa et al, 1999), Mogibacterium pumilum and Mogibacterium vescum (Nakazawa et al, 2000), were included in the present study. Eubacterium infirmum and Eubacterium tardum (Cheeseman et al, 1996), though discussed here, were not examined in this work.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, several novel genera have been proposed for some of the AAGPRs, e.g. Cryptobacterium, Slackia, Eggerthella and Mogibacterium, and some species of Eubacterium have been transferred to the novel genera according to their phylogenetic characteristics (Nakazawa et al, 1999(Nakazawa et al, , 2000Wade et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Taxonomic characterization of Mogibacterium diversum sp. nov. and Mogibacterium neglectum sp. nov., isolated from human oral cavities.

Nakazawa¹,

Poco²,

Sato³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Cited by 167 publications

References 25 publications

Isolation of a Human Intestinal Bacterium Capable of Daidzein and Genistein Conversion

Isolation of a Human Intestinal Bacterium Capable of Daidzein and Genistein Conversion

Rapid Detection of Human Fecal Eubacterium Species and Related Genera by Nested PCR Method

Taxonomic characterization of Mogibacterium diversum sp. nov. and Mogibacterium neglectum sp. nov., isolated from human oral cavities.

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