The Family Methylobacteriaceae

Kelly, Donovan P.; McDonald, Ian R.; Wood, Ann P.

doi:10.1007/978-3-642-30197-1_256

Cited by 51 publications

(41 citation statements)

References 204 publications

(272 reference statements)

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“…Some members of this genus, like Magnetospirullum bellicus, could also grow chemolithotrophically with hydrogen serving as the electron donor (Thrash et al, 2010). Close metabolic association of methanotrophs with Methylobacterium species is well recognized (Kelly et al, 2014). Although various procedures have been described to purify a methanotroph from non-methanotrophic 'satellites' (Dedysh and Dunfield, 2014), none of them appears to be efficient for obligate microaerophilic methanotrophs.…”

Section: Discussionmentioning

confidence: 99%

A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

et al. 2016

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Although representatives with spiral-shaped cells are described for many functional groups of bacteria, this cell morphotype has never been observed among methanotrophs. Here, we show that spiral-shaped methanotrophic bacteria do exist in nature but elude isolation by conventional approaches due to the preference for growth under micro-oxic conditions. The helical cell shape may enable rapid motility of these bacteria in water-saturated, heterogeneous environments with high microbial biofilm content, therefore offering an advantage of fast cell positioning under desired high methane/low oxygen conditions. The pmoA genes encoding a subunit of particulate methane monooxygenase from these methanotrophs form a new genus-level lineage within the family Methylococcaceae, type Ib methanotrophs. Application of a pmoA-based microarray detected these bacteria in a variety of high-latitude freshwater environments including wetlands and lake sediments. As revealed by the environmental pmoA distribution analysis, type Ib methanotrophs tend to live very near the methane source, where oxygen is scarce. The former perception of type Ib methanotrophs as being typical for thermal habitats appears to be incorrect because only a minor proportion of pmoA sequences from these bacteria originated from environments with elevated temperatures.

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Section: Discussionmentioning

confidence: 99%

A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

et al. 2016

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“…While Microvirga and Meganema are putatively methylotrophic, Methylobacteriaceae are facultative methylotrophs and able to grow on methanol and other one-carbon compounds as sources of energy and carbon [53]. Indeed, this group was more abundant in southern streams, with low oxygen, high conductivity, and larger stream areas (Fig.…”

Section: Contrastingly Environment 1 Harbored Representatives Of Mosmentioning

confidence: 99%

Abundance and biogeography of methanogenic and methanotrophic microorganisms across European streams

Nagler

Praeg

Niedrist

et al. 2019

Preprint

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Background: Globally, streams emit significant amounts of methane, a highly potent greenhouse gas. However, little is known about the stream sediment microbial communities that control the net methane balance in these systems, and in particular about their distribution and composition at large spatial scales. This study investigated the diversity and abundance of methanogenic archaea and methane-oxidizing microorganisms across 16 European streams (from northern Spain to northern Sweden and from western Ireland to western Bulgaria) via 16S rRNA gene sequencing and qPCR. Furthermore, it examined environmental factors influencing both abundance and community composition and explored the link to measured potential methane production and oxidation rates of the respective sediments. Results: Our results demonstrated that the methanogenic and methanotrophic microbiomes of the studied European streams were linked to both the temperature and degree of anthropogenic alteration. The microbiomes could be separated into two to three groups according to environmental factors at both stream and catchment scales. Main methanogenic taxa found within more anthropogenically-altered, warm, and oxygen-poor environments were either Methanospirillum spp. or members of the families Methanosarcinaceae and Methanobacteriaceae . Within such environments, methane oxidizing communities were strongly characterized by members of the family Methylobacteriaceae ( Meganema spp. and Microvirga spp.). Contrastingly, communities in colder environments rich in oxygen and with relatively little anthropogenic impact at the catchment scale were characterized by the methanogenic Methanosaetaceae , Methanocellaceae and Methanoregulaceae and the methanotrophic Methyloglobulus spp ., members of the CABC2E06 group (all Methylococcaceae ) and by various Candidatus Methanoperedens. Overall, diversity of methanogenic archaea increased with increasing water temperature. Methane oxidizing communities showed higher diversities in southern sampling sites and in streams with larger stream areas and widths. Potential methane production rates significantly increased with increasing abundance of methanogenic archaea, while potential methane oxidation rates did not show significant correlations with abundances of methane oxidizing bacteria, presumably due to the more diverse physiological capabilities of this group. Conclusions: We present the first large scale overview of the large-scale microbial biogeography of two microbial groups driving the methane cycle dynamics within stream sediments and deduce the impact that future anthropogenic alterations may cause.

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“…These techniques have been employed primarily for the detection, isolation, and culture of species of Methylobacterium (PPFMs), which are found in abundance on plant leaf and stem surfaces, and on which there is an extensive literature [49,[72][73][74]. Numerous species and strains of Methylobacterium have been isolated from plants [49,75].…”

Section: Isolation Of Methylotrophs From Plants Leaves and Stems (Thementioning

confidence: 99%

“…The most environmentally diverse and exhaustively studied of the non-methanotrophic methylotrophs are Methylobacterium species, which comprise the 'aerobic Gram-negative pinkpigmented facultative methylotrophs' (PPFMs). At the time of writing, at least 49 species names had been validated [47][48][49], and their diversity and properties have been comprehensively reviewed [51]. They are facultatively heterotrophic and will grow on methanol and variously on a range of other C 1 compounds, including MMA, using the serine pathway [50].…”

Section: Methylotrophic Aerobic Proteobacteria Found In Soil and Watermentioning

confidence: 99%

“…Numerous species and strains of Methylobacterium have been isolated from plants [49,75]. A standard procedure for the recovery of PPFMs is to make leaf impressions by pressing leaves on to the surface of plates of NMS-methanol agar medium supplemented with cycloheximide at 100 mg l À1 (to inhibit fungal growth) for 1-10 min before removing the leaves.…”

Section: Isolation Of Methylotrophs From Plants Leaves and Stems (Thementioning

confidence: 99%

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Cultivation of Methylotrophs

Kelly

Ardley

Wood

2014

Springer Protocols Handbooks

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Methylotrophy is the ability of numerous Alpha-, Beta-, and Gammaproteobacteria, Gram-positive bacteria, Actinomycetes, and yeasts to obtain all the energy and carbon required for growth from the metabolism of methyl compounds which have no carbon-carbon bonds. Numerous media and techniques for the isolation and culture of methylotrophs have been described, and a comprehensive description of these protocols is given, with substrates such as methanol, methylamines, and methylated sulfur compounds, and the description of some more complex media. The metabolic and habitat diversity of methylotrophs is outlined, ranging from soil and freshwater organisms, through thermophiles and yeasts, to animal hosts and plant colonisers, including specialised types that induce (and reside in) root nodules in some plant species. The methods for the commercial exploitation of some methylotrophs are outlined. This survey excludes the methanotrophs, growing primarily on gaseous methane, as the cultivation of these is considered elsewhere.

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The Family Methylobacteriaceae

Cited by 51 publications

References 204 publications

A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

Abundance and biogeography of methanogenic and methanotrophic microorganisms across European streams

Cultivation of Methylotrophs

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